Agilent Technologies 1290 Infinity II 2D-LC User Manual page 36

2 Concepts of 2D-LC
Active Solvent Modulation (ASM)
Example: ASM with HILIC in
In this example, a HILIC separation was run in the first dimension and a reversed
phase separation in the second dimension. If sample cuts are transferred to the
second dimension, 40 µL of high organic solvent are brought to a reversed phase
column.
Figure 15 Analysis of pesticides using a HILIC separation with high organic solvent composition in
2D resolution with conventional valve
The high elution strength of
with broad and distorted peaks in the left
Figure 16 Conventional analysis of Cut#3 using a reversed
phase separation in
1 1
H
Bonus RP (50 x 2.1 mm, 1.8 µm), H
acetonitrile, 400 µL/min, EICs from conventional 2D-LC (undiluted)
2D-LC User Guide
1
1
D solvent causes bad separation
2
D chromatogram.
2
D
D analysis of pesticides using:
O; B = ACN, Gradient: 100 to 95% acetonitrile in 5 min, 500 µL/min. MHC with 40 µL loops.
2
1
D and reversed phase in
2D resolution with ASM valve
In the right 2D chromatogram a 2D-LC Valve ASM was used
instead of a conventional 2D-LC valve. Peaks are resolved and
the sensitivity is increased.
Figure 17 ASM analysis of Cut#3 using a reversed phase
separation in
1
D: Zorbax RX-SIL (150 x 2.1 mm ID, 5 µm), A = 10 mM NH
O/acetonitrile gradient (0.2% formic acid), weak solvent 3%
2
2
D
2
D
1
D
Ac in
4
2
D :
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