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Chapter 4 Thermal cycle the chips
4
Perform the PCR
Perform the PCR
36
CAUTION! PHYSICAL INJURY HAZARD. During operation, the sample block can
be heated to 100°C. Before performing the following procedure, be sure to wait until
the sample block reaches room temperature.
IMPORTANT! Thermal cycle prepared chips within 2 hours after loading them.
IMPORTANT! Thermal cycling must be performed on a ProFlex
System or a Dual Flat Block GeneAmp
guide. No other thermal cyclers can be substituted at the time of the release of this
manual.
1. Open the heated cover of the thermal cycler and wipe the surface of both
sample blocks using a low-lint wipe to ensure that they are clean and dry.
2. Confirm that the Tilt Base is installed and Chip Adapters are installed in both
sample blocks (even if you are using only one block).
3. Place the chips onto the sample block so that the fill ports on the chips are
positioned toward the front of the thermal cycler. The fill port must be elevated
during thermal cycling to ensure that any bubbles float to the top of the case.
X0300036
Front of instrument
IMPORTANT! If you are thermal cycling less than 24 chips, load according to
the following guidelines:
·
Load the right sample block first, placing at least 1 chip on the right sample
block.
·
Balance the load between the left and right blocks so that the pressure
applied by the heated cover and thermal pads is uniform across all of the
loaded chips.
™
PCR System 9700 as described in this
24x
CAUTION HOT
QuantStudio
™
2 × Flat PCR
™
3D Digital PCR System User Guide
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