Fill The Chip Case With Immersion Fluid - Thermo Scientific Applied Biosystems QuantStudio User Manual

Fill the chip case with Immersion Fluid

QuantStudio ™ 3D Digital PCR System User Guide
5. Seal the chip:
a. Remove the red protective film from the chip lid, then carefully place it onto
the prepared chip so that the window in the lid aligns with the chip.
IMPORTANT! Before applying the chip lid, make certain that it is correctly
oriented to the chip assembly.
b. Pinch together all four sides and corners of the chip case for at least
10 seconds to seal it. While pressing, apply some lateral pressure to ensure
a tight seal.
Note: You can remove the chip from the loading surface when you apply
pressure to seal it.
Proceed to filling the chip case with Immersion Fluid.
1. Hold the chip and lid assembly by its edges at a 45° angle so that air can
escape from the fill port as you fill it.
2. Hold back the top half of the chip lid label to expose the fill port.
Figure 4 QuantStudio
Appendix E Load chips manually
Fill the chip case with Immersion Fluid
™
3D Digital PCR Chip Lid v2
E
>10 sec.
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