About The Chip - Thermo Scientific Applied Biosystems QuantStudio User Manual

Chapter 1 Product information
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About the chip

About the chip
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The QuantStudio 3D Digital PCR 20K Chip v2 is a 10-mm
plate that has a single array of 20,000 reaction wells. Hydrophobic coatings on the
chip surface enable loading and isolation of independent PCR reactions within the
wells.
Once loaded with a reaction, the chip must be sealed using the QuantStudio
Digital PCR Chip Lid v2 and filled with Immersion Fluid. Figure 2 shows the
components of the chip assembly.
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Figure 2 QuantStudio ™
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QuantStudio 3D Digital PCR Chip Lid v2—The lid used to seal the chip for thermal cycling
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and imaging.
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QuantStudio 3D Digital PCR 20K Chip v2—A 10-mm
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the individual PCR reactions.
Chip case—The thermal-conductive base that secures and protects the chip.
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Chip ID—A unique alphanumeric ID on the chip lid.
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Fill port—The hole in the chip case lid through which Immersion Fluid is injected onto the
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chip.
Reaction wells—The 20,000 physical holes on the surface of the chip that contain the
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individual PCR reactions.
2D barcode—A barcode that encodes the same alphanumeric string as the Chip ID, scanned
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by the QuantStudio 3D Digital PCR Instrument for chip tracking.
3D Digital PCR 20K Chip v2
QuantStudio
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high-density reaction
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chip with 20,000 wells that contain
™ 3D Digital PCR System User Guide
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3D
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