Thermo Scientific Applied Biosystems QuantStudio User Manual page 34

Chapter 4 Thermal cycle the chips
4
Prepare the thermal cycler
34
™
– GeneAmp
PCR System 9700—The version number displayed in the main
menu must be 3.12 or later.
08:00 AM
GeneAmp® PCR System 9700
Version: 3.12
Name:tc001 User:<<pe>>
Run Create
F1 F2
v3.12 or later
1
Note: If the GeneAmp
contact technical support to upgrade the instrument firmware before thermal
cycling chips (see "Customer and technical support" on page 123).
• Confirm that the thermal cycler is programmed with the method for thermal
cycling chips. If missing, create the PCR method before proceeding.
Note: See the ProFlex
™
GeneAmp PCR System 9700 Base Module User Manual (Pub. No. 4303481) for
more information on programming methods.
IMPORTANT! The heated cover MUST be at 70°C before loading the chips.
Excessive heat from the heated cover may cause the chips to leak during
thermal cycling.
™
Table 2 ProFlex 2 × Flat PCR System PCR Method
Stage 1 Stage 2
96.0°C 60.0°C
1.6°C/sec 1.6°C/sec 1.6°C/sec 1.6°C/sec
0:10:00 0:02:00
1x (Hold) 39x (Cycles)
[1]
33 nL for firmware earlier than v1.1.4. The reaction volume on the instrument display does not refer to
the reaction volume on the chip and should not be changed.
™
Table 3 GeneAmp
Stage 1 Stage 2
96.0°C 56.0°C
10 min 2 min
1x (Hold) 39x (Cycles)
[1]
The reaction volume on the instrument display does not refer to the reaction volume on the chip and
should not be changed.
4/25/13 25.0°C
Edit Util User
F3 F4 F5
™
PCR System 9700 version is earlier than 3.12,
™
PCR System User Guide (Pub. No. MAN0007697) or the
PCR protocol
Stage 3
98.0°C 60.0°C
0:00:30 0:02:00
1x (Hold)
PCR System 9700 PCR Method
PCR protocol
Stage 3
98.0°C 60.0°C
30 sec 2 min
1x (Hold)
QuantStudio
1
Cover
temp.
10.0°C
1.6°C/sec
70.0°C
∞
Run
speed
10.0°C
∞ Standard
™ 3D Digital PCR System User Guide
Reaction
volume
[1]
1 nL
Reaction
volume
[1]
20 μL