Chapter 4 Thermal Cycle The Chips; Materials Required; Prepare The Thermal Cycler - Thermo Scientific Applied Biosystems QuantStudio User Manual

4

Materials required

Prepare the thermal cycler

32
■
Materials required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
■
Prepare the thermal cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
■
Guidelines for handling loaded chips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
■
Perform the PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
■
Unload the thermal cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
• Loaded and sealed QuantStudio
• ProFlex ™
2 × Flat PCR System (or Dual Flat Block GeneAmp
9700)
™
• QuantStudio
3D Digital PCR Chip Adapters
™
• QuantStudio
3D Digital PCR Thermal Pads
™
• QuantStudio
3D Digital PCR Tilt Base
• Isopropanol
• Gloves, powder-free, nitrile
• Low-lint polyester wipes, clean-room grade
Before running each batch of chips, perform the following to ensure efficient thermal
cycling:
• Confirm that clean QuantStudio
use.
IMPORTANT! If a Thermal Pad becomes dirty, clean the pad using a low-lint
wipe sprayed with isopropanol, then let it dry. Fluids, debris, and other
contaminants that adhere to the pad can transfer to the chips during thermal
cycling and fluoresce during imaging.
IMPORTANT! Use only Thermal Pads that are approved for use with chips.
Thermal compression pads from other PCR applications may not apply uniform
pressure or heat distribution to all chips on the sample block.
Thermal cycle the chips
™
3D Digital PCR 20K Chip v2
™
3D Digital PCR Thermal Pads are available for
QuantStudio
™
PCR System
™ 3D Digital PCR System User Guide