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Chapter 3 Prepare the digital PCR reaction and load the chips
3
Load the chips using the QuantStudio
Prepare the
syringe
containing the
Immersion
Fluid
26
™
3D Digital PCR Chip Loader
1. Remove the Immersion Fluid syringe, plunger, and tip from the packaging.
2. Before uncapping the syringe, gently pull back the plunger 1-2 mm and release
it to break any resistance that may have formed during storage.
3. Remove the cap from the syringe, then attach the syringe tip by pushing it into
place.
IMPORTANT! No twisting or screwing is required to attach the tip.
4. Carefully depress the plunger until Immersion Fluid flows from the tip of the
assembled syringe. Place it on a clean surface and proceed to the next steps.
IMPORTANT! Confirm that the tip is locked firmly in place before proceeding.
IMPORTANT! Open only one syringe at a time. Use all of the Immersion Fluid
within 60 minutes of uncapping the syringe. After a syringe is opened, you
cannot recap it for later use.
1
2
Immersion Fluid syringe
1
Cap (remove)
2
Syringe tip (push to attach)
3
Low-lint wipe
4
<1 hour
3
4
QuantStudio
™
3D Digital PCR System User Guide
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