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Chapter 6 Troubleshooting
6
Troubleshooting chip images using the Chip View
Observation
58
Possible Cause
Condensation may have
been present on the chip
during imaging.
The loading blade was not
seated correctly within the
loader arm.
QuantStudio
Action
IMPORTANT! Condensation may
not be visible on the chip.
• If condensation is not visible – If
possible, read the chip again
after it has warmed to room
temperature. Once equilibrated,
the data quality, flag, and
separation of amplified versus
non-amplified peaks should
improve noticeably.
• If condensation is visible – Using
a low-lint wipe, remove any
condensation or Immersion Fluid
from the surface of the chip by
wiping in one direction. If
needed, use a low-lint wipe
sprayed with isopropanol to
remove any dried residue.
Before pressing the loading button,
confirm that the loading blade is firmly
seated and flush against the loader
arm.
™
3D Digital PCR System User Guide
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