Thermo Scientific Applied Biosystems QuantStudio User Manual page 72

Appendix A Install the QuantStudio
A
™
Install the ProFlex 2 × Flat PCR System
72
™ 3D Digital PCR System
2. Connect the power cord, power on the ProFlex
start (about 45 seconds). Follow the onscreen instructions to complete the
installation.
™
The ProFlex PCR System is ready to use when the Main Menu displays.
IMPORTANT! If the instrument does not start, check the position of the sample
block module. The instrument cannot be powered on if the module is seated
incorrectly or if the lever is not completely closed. If the instrument does not
start even after adjusting the sample block module, or if the screen contains any
permanent patterns of lines or bars, contact technical support for assistance.
3. Configure the ProFlex
System User Guide (Pub. No. MAN0007697).
4. For optimal results, create a new method from the existing 3D template supplied
™
with the ProFlex PCR System and verify the pre-defined method for thermal
cycling the chips:
™
Table 4 ProFlex 2 × Flat PCR System PCR Method
Stage 1 Stage 2
96.0°C 60.0°C
1.6°C/sec 1.6°C/sec 1.6°C/sec 1.6°C/sec
0:10:00 0:02:00
1x (Hold) 39x (Cycles)
[1]
33 nL for firmware earlier than v1.1.4. The reaction volume on the instrument display does not refer to
the reaction volume on the chip and should not be changed.
™
PCR System settings as described in the ProFlex
PCR protocol
Stage 3
98.0°C 60.0°C
0:00:30 0:02:00
1x (Hold)
QuantStudio
™
PCR System, then wait for it to
Cover
temp.
10.0°C
1.6°C/sec
70.0°C
∞
™ 3D Digital PCR System User Guide
™
PCR
Reaction
volume
[1]
1 nL