Related Manuals for Thermo Scientific Dionex IonPac AS31
Summary of Contents for Thermo Scientific Dionex IonPac AS31
- Page 1 Dionex IonPac AS31 Columns 155007 Revision 01 • December 2018 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Product Manual Dionex IonPac AS31 Analytical Column Analytical Column, 2 × 250 mm (Item # 303147) Dionex IonPac AG31 Guard Column Guard Column, 2 × 50 mm (Item # 303148) Thermo Scientific Product Manual for IonPac AS31 Columns Page 2 of 45 155007-01 For Research Use Only.
- Page 3 Sale shall govern all conflicting information between the two documents. For Research Use Only. Not for Use in Diagnostic Procedures. Revision History: Revision 01, December 2018, Original Publication. Thermo Scientific Product Manual for IonPac AS31 Columns Page 3 of 45 155007-01...
- Page 4 IMPORTANT test results; or might contain information that is critical for optimal performance of the system. Highlights helpful information that can make a task easier. Thermo Scientific Product Manual for IonPac AS31 Columns Page 4 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 5: Table Of Contents
Isocratic Elution With and Without a Guard Column ..................19 Effect of Temperature on the Dionex IonPac AS31 Selectivity ..............20 Separations of Various Inorganic and Organic Anions on Dionex IonPac AS31 Column ......21 Separation and Detection of 9 Haloacetic Acids, Bromate, and Dalapon using IC Method and EPA Method 557 Laboratory Synthetic Sample Matrix ................ - Page 6 5.4.3 Shortened Retention Times ........................37 5.4.4 Loss of Front-End Resolution ........................38 Spurious Peaks ..............................38 Thermo Scientific Product Manual for IonPac AS31 Columns Page 6 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures.
- Page 7 Column Cleanup Procedure ........................43 Quality Assurance Report ..........................44 Additional Information ............................45 General Information on PEEK™ Tubing ......................45 Thermo Scientific Product Manual for IonPac AS31 Columns Page 7 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 8: Introduction
(HAAs), dalapon, and bromate in drinking water. The Dionex IonPac AS31 column is a low bleed column designed to be used for ion chromatography separations coupled with Mass Spectrometry (IC/MS), or (IC/MS/MS). It is a high capacity column, which allows relatively large injection volumes. - Page 9 – Introduction Table 1 Dionex IonPac AS31/Dionex IonPac AG31 Operating Parameters Standard Maximum Typical Back Pressure Column(s) Flow Rate Flow Rate psi (MPa ), 15°C mL/min mL/min Dionex IonPac AS31 ~ 3000 (20.68) 0.30 0.40 2 mm Analytical column Dionex IonPac AG31 ~ 200 (1.38)
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Page 10: Installation
A minimum of a Dionex High Pressure Ion Chromatography (HPIC) System is recommended when running Dionex IonPac AS31 columns due to the higher backpressures generated at typical operational flow rates. Systems should have the capability to operate up to at least 5000 psi. -
Page 11: System Void Volume
When making any tubing connections, first turn off the pump. This will avoid any slippage IMPORTANT of the ferrule under high pressure conditions. Thermo Scientific Product Manual for IonPac AS31 Columns Page 11 of 45 155007-01... -
Page 12: Eluents
CAUTION Dionex IonPac Guard Columns A Dionex IonPac AG31 Guard Column is normally used with the Dionex IonPac AS31 Analytical Column. A guard is placed in front of the analytical column to prevent sample contaminants from damaging the analytical column. It is easier to clean or replace the guard column than it is the analytical column. -
Page 13: Sample Concentrators
Dionex IonPac Concentrator Columns are designed for use with specific eluent systems. Use only concentrator columns designed for the eluent system you are using. CAUTION Thermo Scientific Product Manual for IonPac AS31 Columns Page 13 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 14: Operation
For assistance, visit Unity Lab Services online at www.unitylabservices.com. From the U.S., call the Customer Care Center for Dionex Products at 1-800-346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office. NOTE Thermo Scientific Product Manual for IonPac AS31 Columns Page 14 of 45 155007-01... -
Page 15: General Operational Precautions
Take care not to exceed the maximum operational flow rate and pressure of the column. • If the pressure approached the maximum allowed pressure, reduce the operational flow rate. Table 2 Operational Limits for the Dionex IonPac AS31 Column Eluent pH Between 0 and 14 Sample pH Between 0 and 14 Maximum Flow Rate for 2 mm columns 0.40 mL/min... -
Page 16: Eluent Preparation
NOTE and water. Never add the acetonitrile directly to the basic carbonate or hydroxide eluent solutions. SAFETY Thermo Scientific Product Manual for IonPac AS31 Columns Page 16 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 17: Regenerant Preparation For The Thermo Scientific Dionex Chemically Regenerated Suppressor (Dionex Crs 500)
(Document No. 031727) for operating instructions. Recommendations for Method Development The Dionex IonPac AS31 is designed for the determination of haloacetic acids in less than 35 minutes using a hydroxide gradient eluent delivered by a Dionex Eluent Generator. In any type of gradient elution system, it is important to use eluents that produce a minimum shift in baseline conductivity during the run as well as a fast equilibration time from one run to the next. -
Page 18: Example Applications
The Dionex IonPac AS31 is designed for the determination of haloacetic acids using a hydroxide gradient eluent delivered by an Eluent Generator. In any type of gradient elution system, it is important to use eluents that produce a minimum shift in baseline conductivity during the run as well as a fast equilibration time from one run to the next. -
Page 19: Isocratic Elution With And Without A Guard Column
By using this eluent, common inorganic anions can be used to test the performance (QAR) of the Dionex IonPac AS31 column. The Dionex IonPac AS31 analytical column should always be used with the Dionex IonPac AG31 guard column. An operating temperature of 15 °C is used to ensure reproducible resolution and retention. Note that the Dionex IonPac AG31 guard column is packed with a microporous resin of proportionally lower capacity and contributes approximately 1.5% increase in retention time when placed in-line prior to the analytical column under isocratic test... -
Page 20: Effect Of Temperature On The Dionex Ionpac As31 Selectivity
4.3 Effect of Temperature on the Dionex IonPac AS31 Selectivity The following chromatograms demonstrate the effect of temperature on the Dionex IonPac AS31 selectivity. Notice there is a greater effect on the divalent anions (sulfate and thiosulfate) relative to monovalent anions (fluoride, chloride, bromide, and nitrate) when temperature is changed from 15ºC to 30ºC. -
Page 21: Separations Of Various Inorganic And Organic Anions On Dionex Ionpac As31 Column
4.4 Separation of Various Inorganic and Organic Anions The Dionex IonPac AS31 column provides excellent separation of a variety of environmental anions including inorganic anions, oxyhalides, oxyanions, and organic acids. When developing a method, keep in mind that if there is a big difference in the hydrophobicity and valencies of the two analytes, variation in column capacities will have significant effect on the peak resolution. -
Page 22: Separation And Detection Of 9 Haloacetic Acids, Bromate, And Dalapon Using Ic Method And Epa Method 557 Laboratory Synthetic Sample Matrix
4.5 Separation and Detection of 9 Haloacetic Acids, Bromate, and Dalapon using IC Method and EPA Method 557 Laboratory Synthetic Sample Matrix The following chromatograms demonstrate the Dionex IonPac AS31 column’s selectivity and capacity using IC method and EPA Method 557 LSSM matrix. -
Page 23: Analysis Of Municipal Drinking Water Using Dionex Ionpac As31 Column
– Example Applications 4.6 Analysis of Municipal Drinking Water using Dionex IonPac AS31 column The following chromatograms show the analyses of municipal drinking water with and without a spike of 9HAAs. Figure 5 Analysis of Municipal Drinking Water Spiked with Nine Haloacetic Acids... -
Page 24: Separation And Detection Of 9 Haas, Bromate, And Dalapon Using Ic-Ms/Ms
Please note that IC analysis was performed on a Thermo Scientific Dionex ICS-5000 HPIC System and matrix ions were diverted to waste, with only the ions of interest sent to the Mass Spectrometer. -
Page 25: System, Conditions, And Consumables
– Example Applications 4.7.1 System, Conditions, and Consumables Thermo Scientific Dionex ICS-5000 with Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Column: Dionex IonPac AG31/AS31 (2 mm) Eluent: KOH Gradient (see timed events) Timed Events Suppressor: Dionex ADRS 600, 2mm, external water, 0.3 mL/min... -
Page 26: Example Chromatograms
162.854 – 162.856 1.20E5 9. BDCAA 206.804 – 206.807 1.55E5 10. DBCAA 250.753 – 250.758 1.19E5 11. TBAA Minutes Thermo Scientific Product Manual for IonPac AS31 Columns Page 26 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 27: Signal To Noise Comparison
0.3 mL/min. The matrix diversion valve switches the regen mode between external water and recycle modes to provide continuous flow of water to the suppressor and mass spectrometer during matrix diversion. Thermo Scientific Product Manual for IonPac AS31 Columns Page 27 of 45 155007-01 For Research Use Only. - Page 28 IC is made directly to the mass spectrometer electrospray probe. However, if the connection is made through a grounded adapter present on the MS, there is no need for NOTE another ground. Thermo Scientific Product Manual for IonPac AS31 Columns Page 28 of 45 155007-01...
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Page 29: Troubleshooting
Column Contamination Clean Column 5.2.4 Insufficient Equilibration Extend Equilibration Time at 5.4.3 the Start of the Gradient Run Thermo Scientific Product Manual for IonPac AS31 Columns Page 29 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 30: High Back Pressure
5.1.1 Finding the Source of High System Pressure Total system pressure for the Dionex IonPac AG31 Guard Column plus the Dionex IonPac AS31 Analytical Column when using the test chromatogram conditions should be less than 3800 psi. If the system pressure at the standard flow rate is higher than the maximum operational pressure for the system, it is advisable to determine the cause of the high system pressure. -
Page 31: Replacing Column Bed Support Assemblies
E. Screw the end fitting back onto the column. Tighten it finger-tight, then an additional 1/4 turn (25 in x lb). Tighten further only if leaks are observed. F. Reconnect the column to the system and resume operation. Thermo Scientific Product Manual for IonPac AS31 Columns Page 31 of 45 155007-01 For Research Use Only. -
Page 32: Filter Eluent
Dionex CR-ATC 500 or CR-ATC 600 and/or refer to the Clean-Up Procedure, in the Dionex CR-TC Product Manual (Document No. 079684). Thermo Scientific Product Manual for IonPac AS31 Columns Page 32 of 45 155007-01 For Research Use Only. -
Page 33: A Contaminated Guard Or Analytical Column
Inconsistent Retention Times Inconsistent or shifting retention times could be due to one or several different factors. These should be checked to determine the cause and address the issue. Thermo Scientific Product Manual for IonPac AS31 Columns Page 33 of 45 155007-01 For Research Use Only. -
Page 34: Drifting To Shorter Retention Time
When an injection valve is actuated, the timing is critical for consistent retention times. This will occur when the injection valve needs to be cleaned or serviced. Refer to the Operator’s Manual for troubleshooting and service procedures. Thermo Scientific Product Manual for IonPac AS31 Columns Page 34 of 45 155007-01 For Research Use Only. -
Page 35: Poor Peak Resolution
Figure 11 below. Before connecting the fittings, it is recommended to inject water into the cavities of the fluidic system using a syringe or a micropipette while the flow Thermo Scientific Product Manual for IonPac AS31 Columns Page 35 of 45 155007-01 For Research Use Only. - Page 36 Refer to the Suppressor Product Manual for cleaning protocols. Thermo Scientific Product Manual for IonPac AS31 Columns Page 36 of 45 155007-01 For Research Use Only.
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Page 37: Analyte Specific Efficiency Loss
Use one reservoir containing the correct eluent composition to see if this resolves the problem. There may be a problem when one of the proportioned eluents is NOTE less than 5%. Thermo Scientific Product Manual for IonPac AS31 Columns Page 37 of 45 155007-01... -
Page 38: Loss Of Front-End Resolution
Small baseline disturbances at the beginning or at the end of the chromatogram can be overlooked so long as they do not interfere with the quantification of the peaks of interest. Thermo Scientific Product Manual for IonPac AS31 Columns Page 38 of 45 155007-01 For Research Use Only. -
Page 39: Column Care
6. Column Care 6.1 Recommended Operation Pressures Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum recommended operating pressure for the Dionex IonPac AS31 Column is listed in Sections Error! Reference source not found., “Introduction” and 3.1, “... -
Page 40: Inorganic Chemicals
Solvents and water should be premixed in concentrations which allow proper mixing by the gradient pump and to minimize outgassing. Ensure that all of the inorganic chemicals are soluble in the highest solvent concentration to be used during the analysis. Thermo Scientific Product Manual for IonPac AS31 Columns Page 40 of 45 155007-01 For Research Use Only. -
Page 41: Column Cleanup
< 5% levels and the ionic strength of the eluent to < 50 mM levels to avoid creating high pressure zones in the column that may disrupt the uniformity of the column packing. Thermo Scientific Product Manual for IonPac AS31 Columns... -
Page 42: Choosing The Appropriate Cleanup Solution
Organic solvents can be used alone if the contamination is nonionic and hydrophobic. The degree of nonpolar character of the solvent should be increased as the degree of hydrophobicity of the contamination within the range of acceptable solvents. Thermo Scientific Product Manual for IonPac AS31 Columns Page 42 of 45 155007-01 For Research Use Only. -
Page 43: Column Cleanup Procedure
Reconnect the Dionex IonPac analytical column to the suppressor and if your system was originally configured with a guard column, place the guard column in line between the injection valve and the analytical column. Thermo Scientific Product Manual for IonPac AS31 Columns Page 43 of 45 155007-01 For Research Use Only. -
Page 44: Quality Assurance Report
– Quality Assurance Report 7. Quality Assurance Report Thermo Scientific Product Manual for IonPac AS31 Columns Page 44 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 45: Additional Information
Light Blue Blue and Light blue are very similar in color. Additionally, tubing from different suppliers may differ to that stated above. NOTE Thermo Scientific Product Manual for IonPac AS31 Columns Page 45 of 45 155007-01 For Research Use Only. Not for use in diagnostic procedures.