Maintain The Sequencer; Required Materials; Clean Or Decontaminate The Sequencer; Perform The Sequencer Cleaning Manually - Thermo Scientific Ion GeneStudio S5 User Manual

3
Chapter 3 Instrument operation

Maintain the sequencer

Maintain the sequencer
Required
materials
Clean or
decontaminate the
sequencer
Perform the
sequencer
cleaning manually
16
• Lint-free wipes
• 70% isopropanol
• (Optional) 10% bleach solution
In the event of a spill or leak on or inside the instrument, perform the following steps.
Note: Dispose of all waste in appropriate liquid or solid waste containers.
1. Remove the Ion S5
™
reservoir.
2. Remove the Ion S5
™
3. Inspect the waste and nucleotide reagent bays for liquid.
4. Using absorbent paper soak up as much liquid as possible, then wash the
affected area with 10% bleach solution.
5. Wipe the affected surfaces with 70% isopropanol, then allow to air-dry.
The Ion GeneStudio S5 Sequencers require cleaning before initialization. Cleaning is
normally performed automatically at the completion of the previous sequencing run.
To enable two sequencing runs on a single initialization, you must deselect the
"Enable post-run clean" checkbox for the first sequencing run. The post-run cleaning is
then performed normally after the second sequencing run. However, if the "Enable
post-run clean" checkbox is deselected for the second run, the cleaning is not
performed after either run. If the second sequencing run is not performed, or the
cleaning is not performed automatically after the second run, the instrument will not
allow the subsequent initialization to proceed until a manual cleaning has been
performed.
If an Ion GeneStudio S5 Sequencer is initialized and
• a sequencing run is not started within 24 hours, or
• a run is not started or completed due to a power failure or an abort
do not perform a manual cleaning. An instrument reset run is required before
reinitialization. See "Perform an instrument reset run with an initialized, unused
Sequencing Reagents cartridge" on page 18 for more information.
Wash Solution bottle, then remove and empty the waste
Sequencing Reagents cartridge.
Ion GeneStudio S5 Instrument User Guide