Thermo Scientific Applied Biosystems QuantStudio User Manual page 55

Observation
Observation
QuantStudio ™ 3D Digital PCR System User Guide
Troubleshooting chip images using the Chip View
Possible Cause
A bubble was present in the
loading blade when it was
used to apply the PCR
reaction to the chip.
Possible Cause
• The chip leaked during
thermal cycling or
imaging.
• A large bubble was
present in the chip
(insufficient Immersion
Fluid).
• Excess Immersion Fluid
is present on the chip
lid.
Chapter 6 Troubleshooting
Action
If possible, use the AnalysisSuite
Software to filter the low quality data
points, or discard the chip and run the
sample again.
When loading the loading blades:
• If you are using a manual pipette,
pipette to the first stop.
• Decrease your pipetting speed.
Action
If present, remove excess Immersion
Fluid from the chip lid and run the
chip again.
If possible, use the AnalysisSuite
Software to filter the low-quality data
points.
To minimize leakage:
• Leave a small bubble when filling
chips with Immersion Fluid.
• When covering chips with
Immersion Fluid, do not get fluid
on the sides of the chip base.
6
™
™
55