Agilent Technologies 1100 Series User Manual page 90

5 How to optimize the Detector
Table 17 Optimization Overview, continued
Parameter
5 Setting the slit width
• Use 4 nm slit for normal applications.
• Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance
bands and for high concentrations.
• Use a wide slit (e.g. 16 nm) to detect very low concentrations.
• Optimizing spectral acquisition (DAD only)
• Select spectra acquisition mode according to your needs (see
"Spectrum Settings"
• Set the spectral wavelength range (for colorless samples 190...400 nm
is sufficient).
• Set step to 4 nm for normal use; set small step (and slit width) if high
resolution of spectra with fine structure is wanted.
Typical column Typical peak
length width
T<= 5 cm 0.025 min
10 cm 0.05 min
20 cm 0.1 min
>= 40 cm 0.2 min
Typical flow
rate
Internal column diameter
Figure 51
90
on page 81).
Recommended flow cell
Nano
flow cell
0.01 ... 0.2
ml/min
0.5 ... 1 mm
Choosing a Flow Cell
Impact
• spectral resolution, sensitivity and
Semi-mirco
flow cell
Standard
flow cell
0.2 ... 0.4 0.4 ... 0.4
ml/min ml/min
2.1 mm 3.0 mm
1100 Series DAD and MWD User Manual
linearity.
1 ... 2 ml/min
4.6 mm
High pressure
flow cell for
pressures
above 100 bar
0.01 ... 5
ml/min