Agilent Technologies 1100 Series User Manual page 59

Table 10
Activity
After an installation
When switching between reverse phase
and normal phase (both times)
After an installation
To clean the system when using buffers
After a solvent change
After the installation of normal phase
seals (P/N 0905-1420)
The pump should never be used for priming empty tubings (never let the pump run dry). Use
N O T E
a syringe to draw enough solvent for completely filling the tubings to the pump inlet before
continuing to prime with the pump.
1 Open the purge valve of your pump (by turning it counterclockwise) and set
2 Flush all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
Pump for approximately 10 minutes before starting your application.
1100 Series DAD and MWD User Manual
Choice of Priming Solvents for Different Purposes
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Hexane + 5% Isopropanol
flow rate to 3-5 ml/min.
Using the Detector
Comments
Best solvent to flush air out of the system
Best solvent to flush air out of the system
Alternative to Isopropanol (second choice)
if no Isopropanol is available
Best solvent to re-dissolve buffer crystals
Best solvent to re-dissolve buffer crystals
Good wetting properties
4
59