Contamination; Temperature; Reproducibility; Cycle Time (Between Injections) - Waters ACQUITY UPLC I-Class IVD System Manual

2 Optimizing Performance
caution when choosing the Load Ahead and Loop Offline options. Initiating
them before the highly organic part of the gradient reaches the needle can
leave sample residue in the system. Thus you can forfeit to system downtime
whatever time you gained using the options as you clean the system of the
accumulated residue.
The hydrophobicity and solubility of samples are additional factors to consider
when trying to minimize carryover.
Use additional valve cycles (actuate the valve) if you suspect that sample
Tip:
residue in the valve is causing carryover problems.

Contamination

To minimize contamination, ensure cleanliness during sample preparation.
For example, verify that sample preparation tools are cleaned thoroughly.
Controlling Contamination in Ultra Performance LC/MS and
See also:
HPLC/MS Systems (part number 715001307).

Temperature

Waters recommends you set sample temperature limits when configuring a
method in the data application. Always ensure that your temperature limits
are set in accordance with your analysis method. (Refer to online Help for
instructions.)

Reproducibility

The precision (area reproducibility) is less than 0.5% RSD for injection
volumes between 2.0 and 10.0 µL (see

Cycle time (between injections)

The short run time of a UPLC separation requires efficient use of the time
between analyses.
26 July 7, 2014, 715003736IVD Rev. C
Appendix B, "Specifications").