Waters ACQUITY UPLC I-Class IVD System Manual page 24

2 Optimizing Performance
20 points per second yield higher baseline noise, requiring you to adjust filter
time constants accordingly.
The optimal ACQUITY UPLC flow rate differs from that of a typical HPLC
column. The table below offers operating guidelines for ACQUITY UPLC
columns under both isocratic and gradient conditions. Note that the values
provided are approximations and that optimum performance for your
molecule or separation can occur at a different flow rate.
Optimal flow rates for molecular weight range:
Column size
2.1 × 50 mm
2.1 × 50 mm
2.1 × 50 mm
2.1 × 50 mm
Follow these general recommendations when performing a UPLC analysis:
• Use high quality solvents, buffers, and additives (MS-grade, prefiltered
with a 2 µm filter).
• Use high quality water (ultrapure, prefiltered with a 2 µm filter).
• Always use solvent filters on tubing lines in solvent bottles.
• Filter buffers with a 0.2-µm filter membrane.
• Keep concentrated stock solutions, to use when preparing working
solutions.
• Do not add fresh buffer to old, which can promote microbial growth.
• Make fresh buffer solutions daily.
• Do not block the degasser vent line. Trim the tubing, if necessary.
• Do not submerge the waste or degasser vent lines in liquid.
See also:
UPLC I-Class IVD Binary Solvent Manager Overview and Maintenance
Guide (part number 715003739IVD.)
• Flush buffers from the system, using water, immediately after you are
done with them.
• Use 10-20% organic solvent in water as a storage solvent, if you keep the
system idle for extended periods (longer than 24 hours).
24 July 7, 2014, 715003736IVD Rev. C
Molecular weight
<500
1000
1500
2000
For details about how to route the tubing, see the ACQUITY
Flow rate
600 µL/min
300 µL/min
150 µL/min
100 µL/min