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•
Keep the seal-wash line primed.
•
Prime solvent lines during system startup.
•
Keep all solvent lines primed.
•
Monitor the waste level, to ensure that it is never too high.
•
When starting the binary solvent manager, ensure solvent flows through
pump A and pump B. Do not specify a 0 mL/min flow rate at startup for
either pump.
•
Use the Load Ahead option when you desire a shorter cycle time and
only when the conditions specified in the methods for two consecutive
samples are identical.
•
If you experience problems with carryover, do not use the Load Ahead or
Loop Offline options while you are troubleshooting.
•
When installing or removing a column, always hold the active
preheater's reusable compression fitting in place. Rotate the column or
optional in-line filter to install or remove it. Do not tighten using a
wrench.
Carryover
You observe carryover in chromatographic systems when a previously injected
analyte appears as a peak in the chromatogram of subsequent samples.
Carryover tends to occur when a small amount of analyte remains in the
system after a sample is injected. You can measure carryover by observing
analyte peaks that appear when you run a blank sample immediately after an
analytical sample.
For details about carryover performance, see
See also:
"Specifications".
A common cause of carryover is inadequate washing of the system. Choosing
an appropriate wash solvent can minimize carryover for a particular analysis.
The wash solvent must be strong enough to dissolve any remaining sample
and the wash duration must be long enough to remove the residue from the
system.
Method conditions also affect carryover. Too short a hold-time at the final
conditions of a gradient, especially if the gradient is steep, can fail to remove
all analytes from the system. It is important to completely flush the system
and reequilibrate the column before proceeding to a subsequent analysis. Use
Appendix
July 7, 2014, 715003736IVD Rev. C
Carryover
B,
25
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