Solvent Recommendations; Recommended Solvents - Waters ACQUITY UPLC I-Class IVD System Manual

using a buffer, flush it from the pump by running a wet-prime with at least
five system volumes of HPLC-grade, distilled or deionized water.
To prevent microbial growth during shutdowns lasting longer than 24 h, flush
the pump and solvent lines with a solution of organic solvent/water in which
the organic component is greater than 20%.
Caution:
introducing incompatible buffers, consult the instrument's
accompanying documentation to determine what buffers are compatible.
To avoid salt precipitation, nonvolatile buffer concentrations must not
Tip:
exceed 100 mM.

Solvent recommendations

The ACQUITY UPLC I-Class IVD system was designed for reversed-phase
chromatography and ACQUITY UPLC BEH column chemistries. Waters
evaluated the system's reliability using traditional reversed-phase solvents.
This section lists solvents recommended for the ACQUITY UPLC I-Class IVD
system. Contact Waters Customer Service to determine whether you can use
solvents that do not appear in the list without adversely affecting instrument
or system performance.

Recommended solvents

• Acetonitrile
• Acetonitrile/water mixtures
• Isopropanol
• Methanol
• Methanol/water mixtures
• Water
To avoid damaging a mass spectrometer by unwittingly
Solvent recommendations
July 7, 2014, 715003736IVD Rev. C 83