Reflected Light Simple Polarized Light Observation - Olympus BX-URA2 Instruction Manual

Reflected fluorescence system
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4-3 Reflected Light Simple Polarized Light Observation

}To prepare for simple polarized light observation using the reflected illuminator, perform the operations in paragraph
"Selecting the Light Path" in section 4-2, "Reflected Light Nomarski DIC Observation" on page 40.
1
Observation Procedure
1. Place the specimen on the stage and move the stage to bring the specimen into focus. Simple polarized light observation
is now possible.
2. Adjust the field iris diaphragm until the diaphragm opening circumscribes the field of view.
3. Stopping down the aperture iris diaphragm may increase the contrast somewhat.
42
Switching Between Brightfield and
4
Darkfield Observation
1. Loosen the DIC clamping screw @ at the front of the revolving nose-
piece, and gently pull the DIC prism ² outward until a click is heard.
Tighten the claming screw again.
2. Rotate the turret to disengage the U-MDIC3 DIC mirror unit from the light
path.
Or slide the analyzer/polarizer to disengage it from the light path.
1

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