Polarized Light Observation; Adjustments Before Observation; Adjusting The Optical Axis - Olympus BX53M-P Instructions Manual

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POLARIZED LIGHT OBSERVATION

5-1 Adjustments Before Observation

In polarized light microscopy ultimate performance is not obtainable unless optical adjustments are made correctly.
Always performed the following adjustments before observation. Remove the quarter wave plate and sensitive tint plate
from the light path.
Fig. 19
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Adjusting the Optical Axis

When Using the Intermediate Attachment for orthoscopic
observation (U-OPA)
Make sure the 10X objective is inserted into the primary hole on
CAUTION
the centerable revolving nosepiece.
1. Fully insert the alignment polarizer @, provided with the U-OPA into the
test plate adapter (U-TAD). Tighten its clamping knob. (Fig. 19)
2. Remove the condenser.
3. Insert the rotatable analyzer (U-AN360P) ² into the analyzer slot on the
intermediate attachment. Engage the analyzer, loosen the clamping
screw and set the analyzer's rotation dial ³ to 0°. (Fig. 19)
BX53/BX53M-P
(Figs. 19 & 20)
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