Qiagen PyroMark Q96 ID User Manual page 86

Glossary
Term Description
IUPAC
International Union of Pure and Applied Chemistry. An
organization providing recommendations on organic and
biochemical nomenclature, symbols, terminology, etc.
IUPAC codes
A = Adenine
C = Cytosine
G = Guanine
T = Thymine
R = Purine (A or G)
Y = Pyrimidine (C or T)
M = C or A
Out of phase When one of the alleles is sequenced ahead of the other.
Polymorphism
Genetic variations, broadly encompassing any of the many
types of variations in DNA sequences that are found within
a given population.
Presequencing As the substrate is dispensed into a well (indicated by an
signal "S"), contaminants (such as ATP or PPi) will be converted to
light. Too high a substrate peak indicates that high levels
of a contaminant might be present in, for example,
buffers.
Pyrogram
The graph resulting from a sequencing reaction performed
using Pyrosequencing technology. Each incorporated
nucleotide is shown as a peak in Pyrogram.
9-2
K = T or G
W = T or A
S = C or G
B = C, T, or G (not A)
D = A, T, or G (not C)
H = A, T, or C (not G)
V = A, C, or G (not T)
N = Any base (A, C, G, or T)
PyroMark Q96 ID User Manual 01/2016