Qiagen QIAamplifier 96 User Manual
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Qiagen QIAamplifier 96 User Manual

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QIAamplifier
User Manual
For Molecular Biology Application. Not for use in diagnostic procedures.
QIAGEN GmbH, QIAGEN Strasse 1, D-40724 Hilden
1120412
Sample to
Insight__
March 2020
96
™

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Summary of Contents for Qiagen QIAamplifier 96

  • Page 1 March 2020 QIAamplifier ™ User Manual For Molecular Biology Application. Not for use in diagnostic procedures. QIAGEN GmbH, QIAGEN Strasse 1, D-40724 Hilden 1120412 Sample to Insight__...

  • Page 2: Table Of Contents

    Warranty and liability ................. 8 Safety Information ..................9 Symbols and signal words used ............9 Safety markings on the QIAamplifier 96 ..........10 Technical condition ................11 Requirements for the operating personnel ..........11 Safety instructions, transport, and installation ........12 Safety instructions –...
  • Page 3 Control elements ..................23 QIAamplifier 96 front view ..............23 QIAamplifier 96 rear view ..............24 High Performance Smart Lid (HPSL) ............. 25 The QIAamplifier 96 software ............27 Operation ....................36 Power on self-test ................36 Login screen ..................36 Login ....................
  • Page 4 14.2 Disinfecting the device ..............110 14.3 Firmware update ................110 15 Service ....................111 15.1 Device return information ..............111 15.2 Packing the QIAamplifier 96 ............112 16 Disposal ....................113 Appendix A ..................... 114 Declaration of Conformity ................114 Certificates ....................
  • Page 5 Waste Electrical and Electronic Equipment (WEEE) ........116 Index ......................118 Document Revision History ................. 120 QIAamplifier 96 User Manual 03/2020...

  • Page 6: Introduction

    QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques.

  • Page 7: Conventions

    Safety Information. Intended use of QIAamplifier 96 The QIAamplifier 96 is an end-point thermal cycler. It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reaction.

  • Page 8: Warranty And Liability

    Use of the QIAamplifier 96 other than intended  Improper commissioning, operation and servicing of the device  Modifications of the QIAamplifier 96 without prior consultation with QIAGEN  Unauthorized intervention in the QIAamplifier 96  Operation of the QIAamplifier 96 with faulty safety equipment ...

  • Page 9: Safety Information

    Safety Information For your own safety and to ensure error-free and safe operation of the QIAamplifier 96, please read this chapter carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the...

  • Page 10: Safety Markings On The Qiaamplifier 96

    Do not dispose of in household waste CE marking China RoHS marking Safety markings on the QIAamplifier 96 Damaged or missing safety symbols can cause incorrect actions leading to personal injury or material damage. Important: Do not remove safety symbols. Replace damaged safety symbols immediately.

  • Page 11: Technical Condition

    Technical condition The QIAamplifier 96 has been built and certified according to safety standard EN 61010-1. Do not modify the device in any way. Any modification of the device will lead to a loss of warranty and the EN 61010-1 certificate and poses a potential risk.

  • Page 12: Safety Instructions, Transport, And Installation

    Only ship the QIAamplifier 96 in its original packaging with the transport lock installed.  Please verify that the delivery is complete on receipt of the QIAamplifier 96 and check for  possible transport damage. In case of claims, please contact QIAGEN.
  • Page 13 2.6.3 Safety instructions – electrical equipment Work on the electrical components of the QIAamplifier 96 may only be performed by a qualified electrical technician in accordance with the applicable electrical engineering rules. Life-threatening electrical voltages may occur in the interior of the QIAGEN QIAamplifier 96.

  • Page 14: Behavior During Emergencies

     (unless stated otherwise). Behavior during emergencies In case of danger or accidents, immediately switch off the QIAamplifier 96 using the main switch on the equipment backplate. Disconnect the mains plug from the power supply. QIAamplifier 96 User Manual 03/2020...

  • Page 15: Technical Specifications

    550 watt 100, 115, 230 Volt ± 10%, 50 – 60 Hz Operating voltage Fuse 2 x 8AT (special type of fuse required) Please contact QIAGEN technical services for assistance Noise emissions Very low, max. 45 dBA Interfaces USB A, Ethernet †...

  • Page 16: Function And Setup

    Figure 1. The QIAamplifier 96 Thermal Cycler. The QIAamplifier 96 thermal cycler features an automatic restart. If a power failure occurs during the run, the device will continue the run as soon as power is restored. In case of long-term power failure (longer than 30 minutes), the device will keep the sample block at 4°C (freeze step) and the...

  • Page 17: High Speed Thermoblock

    High speed thermoblock All blocks of the QIAamplifier 96 thermal cycler are made of aluminum and offer high ramp rates for fast protocol run times. A rubber seal on the heated lid forms an encapsulated space around the sample block as soon as the lid is closed. The closed space serves to improve the temperature uniformity of the sample block and avoids the formation of condensed water at the final PCR cooling step.

  • Page 18: User-Specific Quick Start Of Programs

    Linear gradient tool For optimizing new primer pairs in a single run, the QIAamplifier 96 is equipped with a gradient function. Finding the best primer annealing temperature is crucial for the specificity and efficiency of PCR reactions. Often the optimization of experiments is conducted to a limited extent only, leading to unspecific byproducts or reduced PCR sensitivity.

  • Page 19: Glp Compliance

    4.2) the same pressure is always applied to the consumables for absolutely reproducible conditions. The QIAamplifier 96 thermal cycler is not limited to reagents from a specific vendor and can be used with any kind of polymerase. Regardless of whether isothermal, hot start, or non-hot start polymerases are used, the QIAamplifier 96 will produce reliable results.

  • Page 20: Installation

    The QIAamplifier 96 can be operated at 100, 115, or 230 volts. Note: Set the operating voltage by turning the dial on the underside of the QIAamplifier 96 to the desired voltage. You can do so with a coin.
  • Page 21 Figure 1. Voltage selector switch on the underside of the device. QIAamplifier 96 User Manual 03/2020...

  • Page 22: Commissioning

    Place the QIAamplifier 96 on a solid dry surface.  If the QIAamplifier 96 has been moved, allow the device to adjust to room  temperature before switching it on for the first time (1 to 6 hours).

  • Page 23: Control Elements

    Control elements QIAamplifier 96 front view Dial for adjusting the contact pressure Lid sealing button Display USB port Figure 2. Front view of the QIAGEN QIAamplifier 96. QIAamplifier 96 User Manual 03/2020...

  • Page 24: Qiaamplifier 96 Rear View

    QIAamplifier 96 rear view The Ethernet port, mains plug and mains switch are located at the rear of the QIAGEN QIAamplifier Ethernet port Mains switch Fuse holder Mains plug Figure 3. Rear view of the QIAGEN QIAamplifier 96. QIAamplifier 96 User Manual 03/2020...

  • Page 25: High Performance Smart Lid (Hpsl)

    High Performance Smart Lid (HPSL) The QIAamplifier 96 has a height-adjustable lid for optimal contact pressure on the samples. 6.3.1 Closing the lid 1. Place the samples in the block, then close the lid until you feel the button in the front click into position.
  • Page 26 1. Reduce the contact pressure of the lid completely by turning the lid wheel counterclockwise until there is no resistance. Open the lid by pressing the button on the front of the lid. The lid opens and locks in a defined end position.  QIAamplifier 96 User Manual 03/2020...

  • Page 27: The Qiaamplifier 96 Software

    The QIAamplifier 96 software The QIAamplifier 96 thermal cycler features a 7” color touchscreen and a software for operation. 6.4.1 Keyboard For some applications, names, addresses, or passwords have to be defined. For this purpose, the QIAamplifier 96 software offers a keyboard to enter characters, digits, and special characters.
  • Page 28 6.4.2 Keypad In some screens of the QIAamplifier 96, software digits have to be entered. For this purpose, the software features a keypad integrated in some screens: Figure 6. QIAamplifier 96 keypad. The keypad can be used to enter digits, dots, minus signs, and infinite. In addition the following...
  • Page 29 6.4.3 Cursor keys The QIAamplifier 96 software uses lists, tables, or graphical representations. Whenever the number of entries becomes higher than the maximum number that can be displayed on a screen, the software will show cursor keys for navigation. Scroll up...
  • Page 30 6.4.5 List of commands and icons used The QIAamplifier 96 software uses the following icons: Command Icon Home Back Right Left Down Confirm Cancel Login User management New user Delete user Edit user Table continued on next page QIAamplifier 96 User Manual 03/2020...
  • Page 31 Command Icon Incubation Start incubation Programs New program Open template Copy program/copy all programs Delete program Edit program Start program Quick select program or user Stop program Done Save program Table continued on next page QIAamplifier 96 User Manual 03/2020...
  • Page 32 Table continued from previous page Command Icon Pause program Continue program Skip step Graphical view Spreadsheet view Gradient display Edit step Insert step Delete step Time increment Heating rate Gradient Linear Gradient Tool Table continued on next page QIAamplifier 96 User Manual 03/2020...
  • Page 33 Table continued from previous page Command Icon Standard gradient programming Preheating OFF Preheating ON Tools Settings Date and time Automatic user logout Beeper Network Display brightness Display darker Display brighter Touchscreen calibration Table continued on next page QIAamplifier 96 User Manual 03/2020...
  • Page 34 Save run log file to USB Save all run log files to USB View run log file View program Log file self-test Error log file Extended self-test Start extended self-test Stop extended self-test Table continued on next page QIAamplifier 96 User Manual 03/2020...
  • Page 35 Table continued from previous page Command Icon Backup file / Save backup file Load backup file Cycler information QIAamplifier 96 User Manual 03/2020...

  • Page 36: Operation

    Operation Power on self-test After the QIAamplifier 96 is switched on, the device performs an automatic self-test (power on self- test). For the power on self-test, a log file is created and stored in the thermal cycler memory (see chapter 11.3.2).

  • Page 37: Login

    Left or Right cursor keys to turn the page. (See chapter 6.4.3). Tap the Password input field and enter the password using the QIAamplifier 96 keyboard (see chapter 6.4.1). The default password for the Admin administrator is Admin. Passwords are case-sensitive.
  • Page 38 Figure 9. QIAamplifier 96 enter password screen. QIAamplifier 96 User Manual 03/2020...

  • Page 39: Home Screen

    Home screen After user login, the QIAamplifier 96 software shows the home screen. The home screen offers the following functions: Figure 10. QIAamplifier 96 home screen. Command Function Chapter Programs Viewing, running and editing programs See chapter 8 Block Shows the current block status. If a block is free, a touch on the See chapter 7.5 and chapter...

  • Page 40: Block Status

    QIAamplifier 96 software. If the status is Running or Pause, a touch of the button will lead to the program view (see chapter 10.1). If the status is Free, the quick start function is activated (see chapter 7.6).
  • Page 41 Button Block status Color Free Green Running Yellow Pause Blue QIAamplifier 96 User Manual 03/2020...

  • Page 42: Quick Start Function

    Quick start function The QIAamplifier 96 software offers a quick start function for previously used programs. To use the quick start function, press the block status display button in the login screen (see chapter 7.2) or in the home screen (see chapter 7.4).
  • Page 43 (see chapter 8.1). Start Press the corresponding button to activate the programming mode or select a program from the list of templates: Edit New from template QIAamplifier 96 User Manual 03/2020...

  • Page 44: Incubation Mode

    Set whether the heated lid should be on or off and whether it should preheat or not using the corresponding buttons (see Figure 14). Incubate Note: To set the time to endless, use the button. To start incubation, press Start Figure 13. QIAamplifier 96 incubation mode screen. QIAamplifier 96 User Manual 03/2020...
  • Page 45 Note: To stop the incubation, use the corresponding command. Do not switch off the device while the incubation is running. The device will react like after a power failure and will restart the incubation step when it is switched on next time. QIAamplifier 96 User Manual 03/2020...

  • Page 46: Creating, Editing, And Saving Programs

    Creating, editing, and saving programs Programming modes The QIAamplifier 96 software offers spreadsheet or graphical programming. Use the buttons to toggle between the two modes. The following screenshots show the spreadsheet and graphical programming mode. Graphical view Spreadsheet view Figure 14. Spreadsheet programming.
  • Page 47 Step options (go to, temperature increment, time increment and heating rate) If a program has more than 6 steps, use the cursor keys to navigate in the spreadsheet or the graphical display (see chapter 6.4.3) QIAamplifier 96 User Manual 03/2020...

  • Page 48: Creating A New Program/Using A Program Template

    After login, press Program in the home screen (see chapter 7.3). Program The program overview screen opens: Figure 16. QIAamplifier 96 program overview screen. In the program overview screen, press New from template: New from template The program template screen opens (see Figure 19). Search for a program template using the cursor keys and press the corresponding button to select it.
  • Page 49 Note: The QIAamplifier 96 offers several program templates for different purposes. You can either use a template and modify it as desired or select Blank. Blank is a program with no predefined steps (see Figure 18). Spreadsheet programming Graphical programming Figure 17.

  • Page 50: Assigning Program Names

    Assigning program names Each template has a specific name which is superimposed in the programming screen. To enter a name, tap the Name field and enter a new name using the QIAamplifier 96 keyboard (see chapter 6.4.1). Note: If a character is pressed, the pre-displayed name will be deleted and a new program name can be set.

  • Page 51: Preheating The Heated Lid

    Figure 19. QIAamplifier 96 heated lid temperature setting screen. NOTICE The heated lid automatically switches off at a temperature difference of >75 °C between the block and the heated lid. At these low block temperatures, sample condensation on the tube lid is no longer to be expected. In addition, it supports a longer service life of the Peltier elements in the block, since no unnecessary cooling against the heating cover is required.

  • Page 52: Editing Steps

    Editing steps When editing program steps, the following parameters can be entered in the QIAamplifier 96 software: Parameter Description and limits Chapter Temperature Temperature for a step in °C. The target temperature can be set See chapter 8.6 between 3.0°C and 99.0°C in tenths of a degree.
  • Page 53 99.0°C. Use the tab button or activate the next input field and enter a hold time in h:mm:ss. The QIAamplifier 96 software enables several program steps to be programmed subsequently (Multi Step Programming). Use the cursor keys to navigate between program steps.
  • Page 54 Programming loops A typical PCR program consists of repetitive steps for denaturation, annealing, and extension. For the repetition of steps, the QIAamplifier 96 software enables the programming of loops. For a loop two parameters have to be set: 1. The step number to which the program should return (Go To)
  • Page 55 The average ramp rate can be adjusted for each step. This adjustment can be useful for special PCR applications, for example, telomerase PCR. The average ramp rate specifies the speed at which the selected step is reached with. QIAamplifier 96 User Manual 03/2020...
  • Page 56 Figure 21. Temperature gradient on a 96 well sample block In a specific column all wells have the same temperature but the temperature differs from column to column. The temperature gradient can be applied in both directions; the highest temperature can be either in column 1 or column 12. QIAamplifier 96 User Manual 03/2020...
  • Page 57 To program a gradient step press Gradient (see Figure 21). There are two options to program a gradient. To toggle between the two options, press the corresponding button on the QIAamplifier 96 gradient programming screen (see Figure 23): Linear Gradient Tool...
  • Page 58 Figure 22. QIAamplifier 96 Linear Gradient Tool screen. To create a gradient using the standard gradient programming screen, enter a temperature for the first column and the last column in the sample block (see Figure 24). The effective temperatures for all the other columns are automatically calculated and displayed as a bar chart.
  • Page 59 8.6.2 Direct programming of program parameters for a step The QIAamplifier 96 software allows individual parameters (temperature, hold time, loops, temperature, and time increment, as well as the ramp rate) for a step to be edited directly in the graphical or spreadsheet view.
  • Page 60 (see Figure 26). The loop setting is predefined. The activated program option below the graphical view can then be edited by pressing the input field. Figure 25. QIAamplifier 96 graphical program view. Parameter Spreadsheet view ①...
  • Page 61 Figure 26. QIAamplifier 96 individual program parameter editing. You also have the option of programming a gradient step. To do this, when editing the temperature, press the Grad button. Gradient QIAamplifier 96 User Manual 03/2020...

  • Page 62: Inserting A Step

    28). Figure 27. QIAamplifier 96 gradient view. Inserting a step To insert a step, select a program step on the QIAamplifier 96 spreadsheet or graphical programming screen and press Insert. Insert When you press Insert, the screen for editing program steps opens (see chapter 8.6). Enter the desired parameters for the new step.

  • Page 63: Deleting A Step

    Deleting a step To delete a step, select a program step on the QIAamplifier 96 spreadsheet or graphical programming screen and press Delete. Delete Note: If a step is deleted, all the following steps will be moved one position lower. If, for example, step 3 is deleted, step 4 will become step 3, step 5 will become step 4 and so on.
  • Page 64 Figure 28. QIAamplifier 96 program overview screen. There is also the option of selecting a user directory or a program location using the quick select function. Touch the icon above the user or program view (see Figure 29). Quick select function An overview screen opens in which a maximum of 30 users or 50 user programs are displayed.
  • Page 65 Figure 29. QIAamplifier 96 quick select screen Before saving the program there is still the possibility of changing the program name. To do this, press Save as and enter the new program name using the QIAamplifier 96 keyboard (see chapter 8.3).

  • Page 66: Starting, Copying, And Deleting Programs

    The QIAamplifier 96 software offers a quick start function for previously started programs (see chapter 7.6). This chapter describes the process of selecting and starting stored programs from a user account. To select a program to be started, press Programs on the QIAamplifier 96 home screen (see chapter 7.4). Programs The program overview screen opens with the directory of the logged in user preselected (see Figure 31).

  • Page 67: Copying A Program

    To copy a program, touch the corresponding buttons to select a user directory and a program on the QIAamplifier 96 program overview screen (see Figure 31). If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 68: Copying All Programs

    Touch the corresponding buttons to select a user directory and a storage location on the QIAamplifier 96 program overview screen (see Figure 33). The program to be copied is highlighted in green. If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 69: Deleting A Program

    To delete a program, touch the corresponding buttons to select a user directory and a program on the QIAamplifier 96 program overview screen (see Figure 31). If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 70: Deleting All Programs

    To delete all programs, touch the corresponding buttons to select a user directory on the QIAamplifier 96 program overview screen (see Figure 31). If the number of user directories is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 71: Running, Pausing, Continuing, And Stopping Programs

    10.1 Display during a run After a program has been started (see chapter 9.1), the QIAamplifier 96 software can display the currently running program in a spreadsheet or graphical view. In addition, the curve of the gradient can be displayed in gradient steps. Use the following buttons to toggle between the different views.
  • Page 72 Spreadsheet view Figure 36. QIAamplifier 96 display during the run in a graphical, spreadsheet and gradient view. The following parameters are summarized in the headline in the table: Program name  Sample block temperature  Estimated end  Heated lid temperature ...

  • Page 73: Pausing A Program

    During a gradient step, the temperatures present in each individual column in the sample block can be checked in the gradient display. 10.2 Pausing a program To pause a program, press Pause on the QIAamplifier 96 run display screen (see Figure 37). Pause QIAamplifier 96 User Manual 03/2020...
  • Page 74 Figure 37. QIAamplifier 96 run display screen during a program pause. Note: Pressing the Pause button requires manual interaction. The QIAamplifier 96 software also allows pauses to be programmed (see chapter 8.6), then the pause step is automatically started by the device.

  • Page 75: Continuing The Program

    The QIAamplifier 96 will continue the program from exactly the point at which it was paused. 10.4 Skipping a step To skip a program step, press Skip on the QIAamplifier 96 run display screen (see Figure 37). Skip The QIAamplifier 96 will continue with the next program step and skip the current step.
  • Page 76 Figure 38. QIAamplifier 96 confirmation prompt. After confirmation of the confirmation prompt the QIAamplifier 96 stops the current program. QIAamplifier 96 User Manual 03/2020...

  • Page 77: Tools

    11 Tools The QIAamplifier 96 software provides the following tools: Settings Defines basic device settings See chapter 11.1 User Management Tool for user management See chapter 11.2 Documentation Access to stored log files See chapter 11.3 Self Test Executes the extended self-test See chapter 11.4...

  • Page 78: Configuration

    Press the corresponding button to access the desired function. 11.1.1 Setting the date and time The QIAamplifier 96 software allows the user to set the date and time. The date has the format day:month:year and the time the format hours:minutes:seconds.
  • Page 79 Figure 41. QIAamplifier 96 date and time entry screen. Activate the corresponding input field and use the keypad on the right side of the date and time entry screen to enter digits. The following buttons may also be helpful when completing the fields:...
  • Page 80 11.1.2 Automatic user logout The QIAamplifier 96 features an automatic user logout function. The automatic user logout can be switched On or Off by using the corresponding button: Automatic logout On Automatic logout Off If automatic user logout is switched on, the time after which the user is logged out from the system can be set.
  • Page 81 11.1.3 Configuring the beeper The QIAamplifier 96 has a beeper which, if switched on, will issue an acoustic signal when a PCR program is finished. The beeper can be switched ON or OFF by activating the corresponding checkbox. Figure 43. QIAamplifier 96 beeper configuration screen.
  • Page 82 8.6.1.7 Network users The software stores the users who access the QIAamplifier 96 via the network and lists them in chronological order in a table. The function makes it possible to check if only authorized persons have network access to the device.
  • Page 83 Darker Brighter Figure 45. QIAamplifier 96 display brightness adjustment screen. 11.1.6 Screen calibration To calibrate the screen, follow the instructions and touch the screen at the center of the displayed circle. This procedure is repeated three times at different positions on the display. If, at the end of the procedure, a small cross is shown within the large circle, the calibration was successful.

  • Page 84: User Management

    (see chapter 11.6). 11.2 User management The QIAamplifier 96 can manage up to 90 user directories. User management allows new users to be created and user rights to be set. Please observe the following guidelines: QIAamplifier 96 User Manual 03/2020...
  • Page 85 Figure 48. QIAamplifier 96 user management screen. Available users are listed on the QIAamplifier 96 user management screen (see Figure 49) sorted by the date and time of the last login and the username, the initials and the group assignment are shown.
  • Page 86  Figure 49. QIAamplifier 96 create new user screen. 11.2.2 Editing user settings To edit the user settings, select a user on the QIAamplifier 96 user management screen (see chapter 11.2) and press Edit. Edit On the next screen, the user name setting can be changed.
  • Page 87 Figure 50. QIAamplifier 96 user data screen. Note: For the administrator, the user name Admin and the initials ADM cannot be modified. We recommend to change the password for the administrator Admin after the first system start and to create a backup of the system. Folders, programs, users, and rights settings can be restored from a backup file.

  • Page 88: Documentation

    Figure 51. QIAamplifier 96 confirmation prompt. After confirmation of the confirmation prompt, the user and all of his programs will be deleted. 11.3 Documentation The QIAamplifier 96 software provides the following documentation tools: Run Log File Documentation of PCR runs See chapter 11.3.1...
  • Page 89 11.3.1 Run log file The QIAamplifier 96 lists up to 24 run log files for the programs that were last started. Figure 53. QIAamplifier 96 run log file overview screen The run log files are sorted by date and time. The run log file for the program that was last started is shown at the first position in the table.
  • Page 90 To view the corresponding program, press View Program. View program The QIAamplifier 96 offers the option to show the program in graphical from or as a spreadsheet (see chapter 8.1). To toggle between the graphical and spreadsheet view, use these buttons:...
  • Page 91 Graphical view Spreadsheet view Figure 55. QIAamplifier 96 program view screen. Use these buttons to toggle between the overview screen, the run log file screen and the view program screen: Overview Run Log Files View Run Log File Show Program To export all the saved run log files on USB, press Save all Run Log Files on USB.
  • Page 92 Save Run Log File on USB 11.3.2 Power On Logfile With each system start, the QIAamplifier 96 performs an initial self-test and stores the results in a power on logfile. In the power on logfile screen, the following information is listed: Power-on by date and time ...
  • Page 93 The test result can be Passed or Failed and is listed in the last row of the table for each test. 11.3.4 Error log file The QIAamplifier 96 records all errors by date and time. The latest error is shown at the first position in the error table. The error code and the message are also listed.
  • Page 94 Regulation Tests if the sample block is controlled properly The QIAamplifier 96 software will automatically recommend at regular intervals to carry out an extended self-test. Although it is not absolutely necessary to perform the test, we highly recommend to follow the prompt and to let the device check itself.
  • Page 95 To start the extended self-test press: Start Self-Test Figure 60. QIAamplifier 96 extended self-test screen. The software provides a progress overview during the extended self-test. When a test has finished, the result Passed or Failed is displayed. For the currently running test, the status Running is shown and all other tests for which no result is shown are not finished yet.

  • Page 96: Service Info File (Sinf)

    The service info file is a helpful tool for remote fault diagnosis by QIAGEN Technical Service. To create a service info file, connect a USB stick to the QIAamplifier 96 and press Info File to USB. Info File to USB The service info file can be sent through e-mail to QIAGEN Technical Service department.

  • Page 97: Backup

    11.5 Backup The backup function is used to synchronize the memory contents between QIAamplifier 96 thermal cyclers. With the backup all folders, programs, users and user settings can be stored to USB and transferred to another QIAamplifier 96. Figure 62. QIAamplifier 96 backup file screen.

  • Page 98: Cycler Info

    Revision number processor board  Software version processor board  Revision number IO  Software version IO  The information is summarized in two screens. Press System/Cycler Info to toggle between the two screens. System/Cycler Info QIAamplifier 96 User Manual 03/2020...
  • Page 99 Figure 65. QIAamplifier 96 Cycler Info Screen QIAamplifier 96 User Manual 03/2020...

  • Page 100: Contact

    11.7 Contact Whenever you encounter a QIAamplifier 96 error, ensure that you have the following information at hand: Software version (see section xxx).  Sample input material  Detailed description of the error situation  This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue.

  • Page 101: Quick Reference Guide

    Important: Release the lid wheel before opening the lid! Adjust the lid wheel for every individual run. Login After the QIAamplifier 96 has completed the initial self-test, the login screen is displayed: Press Login to log in as an existing user. ...
  • Page 102 Press Logout to log out.  Editing, saving, and copying programs Press Programs on the QIAamplifier 96 home screen. The program overview screen opens:  To edit a template, press New from template, select a template from the list, and press Open ...
  • Page 103 The QIAamplifier 96 programming screen opens. Use the following buttons to toggle between the different programming modes: Graphical mode Spreadsheet mode Gradient mode Spreadsheet programming screen Graphical programming screen Enter a program name. To do this, tap the Name field and enter a name using the ...
  • Page 104 Done. The software opens the screen for copying and storing programs. To save the program, select a user directory and a storage location. Then, press Save. The  program is now saved. QIAamplifier 96 User Manual 03/2020...
  • Page 105 Starting, stopping, and pausing programs The QIAamplifier 96 stores the latest 5 started or edited programs for each user. To quick start  a program, press Quick Start on the QIAamplifier 96 home screen. Select a program from the list and press Start.
  • Page 106 To skip a step, press Skip. The program will continue with the next step.  To stop an active program, press Stop program and confirm the confirmation prompt.  Programs with a pause in the last step have to be stopped manually as well. QIAamplifier 96 User Manual 03/2020...

  • Page 107: Troubleshooting

    13.2 Slow heating and cooling The QIAamplifier 96 is equipped with strong fans that remove the heat from the heat sink. The air supply to these fans is located on the underside of the device. Make sure that the inlet is not clogged by dust or other material (e.g., a piece of paper under the device may be sucked into the fan and...

  • Page 108: Adaptation Of Programs From Other Thermal Cyclers

    13.5 Adaptation of programs from other thermal cyclers Since the QIAamplifier 96 thermal cycler is a very fast device, it may be necessary to reduce the heating and cooling ramp when running protocols from other (slower) thermal cyclers (see section Adjusting the ramp rate).

  • Page 109: Maintenance And Care

    QIAGEN Technical Services. All maintenance work and repairs beyond this scope must only be performed by QIAGEN customer service personnel or authorized and trained persons. Any unauthorized intervention limits warranty entitlements.

  • Page 110: Disinfecting The Device

    Wipe spilled samples or reagents immediately with an absorbent cloth or piece of paper.  If the QIAamplifier 96 is used for the analysis of infectious material, great care must be taken  because the QIAamplifier 96 cannot be decontaminated as a whole device.

  • Page 111: Service

    15 Service If you have any problems with the device, please contact our customer service department or your local QIAGEN dealer. For the address of the QIAGEN customer service department, refer to the inside front cover of this operating manual.

  • Page 112: Packing The Qiaamplifier 96

    QIAGEN uses an extra designed packaging system with inserts of foamed material. Important: The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions are followed. QIAGEN is not liable for transport damage due to incorrect packaging.

  • Page 113: Disposal

    The operator of the QIAamplifier 96 must dispose of any waste materials that occur during measurements (sample materials) in accordance with statutory and local regulations. At the end of its service life, the QIAamplifier 96 and all its electronic components must be disposed of as electronic waste in accordance with valid regulations.

  • Page 114: Declaration Of Conformity

    Appendix A Declaration of Conformity Name and address of the legal manufacturer: QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up-to-date declaration of conformity can be requested from QIAGEN Technical Services. QIAamplifier 96 User Manual 03/2020...

  • Page 115: Certificates

    Certificates For up-to-date certificates, you may request them from QIAGEN Technical Services or from your local QIAGEN representative. QIAamplifier 96 User Manual 03/2020...
  • Page 116 Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE-marked electronic equipment is provided.
  • Page 117 Representations and warranties made by any person, including representatives of QIAGEN , which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN.
  • Page 118 Login screen, 33 Temperature increment, 50 Maintenance, 106 Template, 43 Manual Time increment, 50 Nomenclature, 7 Program preview, 62 Operating voltage, 20 Quick start function, 37 Packaging, 109 Safety instructions, 9 Password electrical equipment, 12 QIAamplifier 96 User Manual 03/2020...
  • Page 119 Factory settings, 79 Calibration, 78 Network, 76 User management Screen calibration, 78 Creating a user, 81 User management, 79 Delete user, 82 Signal words, 9 Editing user settings, 82 Step User Management, 79 Skip, 70 Warranty, 7 QIAamplifier 96 User Manual 03/2020...
  • Page 120 Document Revision History Date Changes March 2020 Initial release QIAamplifier 96 User Manual 03/2020...
  • Page 121 QIAGEN specifically disclaims any other licenses, expressed or implied other than those expressly stated. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.
  • Page 122 Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com QIAamplifier 96 User Manual 03/2020 1120412 HB-2739-001 03/2020...

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