Qiagen QIAxtractor Manuals

Manuals and User Guides for Qiagen QIAxtractor. We have 1 Qiagen QIAxtractor manual available for free PDF download: User Manual

Qiagen QIAxtractor User Manual

Qiagen QIAxtractor User Manual (207 pages)

Brand: Qiagen | Category: Laboratory Equipment | Size: 15 MB

Table of Contents

Table of Contents
2
1 Introduction

10
2 Safety Information

13
- Proper Use
  13
- Electrical Safety
  14
- Biological Safety
  15
  - Samples
    15
- Chemicals
  16
  - Toxic Fumes
    16
- Waste Disposal
  16
- Mechanical Hazards
  16
- Maintenance Safety
  17
- Symbols on the Qiacube HT
  18
3 General Description

19
- The Qiacube HT
  19
  - Instrument Lid
    20
  - UV Lamp
    20
  - Pipetting Head
    21
  - Tip Chute
    21
  - HEPA Filter
    21
  - Accessory Panel
    22
  - Feet
    22
  - Worktable
    22
  - Principles of Operation
    23
- Instrument
  25
4 Installation Procedures

32
- Instrument Delivery and Installation
  32
- Requirements
  32
  - Site
    32
  - Unpacking the Instrument
    33
  - AC Power Connection
    33
  - Hardware Installation
    34
    
    HEPA Filter System
    34
    
    UV Lamp
    34
    
    Vacuum Control Station
    34
    
    Pinch Valve
    35
    
    Vacuum Waste Hose
    35
  - Software Installation
    37
    
    Installing the Qiacube HT Software
    37
  - Connecting the Instrument to the Laptop
    43
    
    Connecting the Computer to the Qiacube HT
    43
  - Hardware Wizard
    44
  - Configuring the COM Port
    45
  - Important Notes before First Use
    46
5 Qiacube HT Software

47
- Mode
  47
- Normal" Mode
  47
- Virtual" Mode
  47
- Starting the Qiacube HT Software
  48
- The Software Workspace
  51
- Virtual" Mode Indicator
  52
  - Menu Bar
    52
  - Toolbar
    52
  - Workspace Items
    53
  - Right-Hand Pane
    53
  - Status Bar
    54
- Menus
  54
- File" Menu
  54
- Control" Menu
  55
- Wizards" Menu
  55
- Options" Menu
  56
- Help" Menu
  56
- Toolbar
  58
- Right-Hand Pane
  60
- Workspace Items
  61
- Plates
  61
  - Plate Properties
    61
  - Choosing the Plate Type
    62
  - Defining Favorite Plate Types
    63
  - Changing Plate Function
    64
- Tips
  65
  - Tip Properties
    65
  - Tip Availability
    66
  - Reagents
    68
    
    Reagent Properties
    68
    
    Configuring Reagents
    69
    
    Required Reagent Volume
    70
  - Samples and Reactions
    71
    
    Sample Properties
    72
    
    Reaction Properties
    73
    
    Common Properties and Operations
    76
    
    Entering Sample Bar Codes
    76
- Data Transfer Formats
  77
- Importing Sample and Reaction Data
  78
- Exporting Sample and Reaction Data
  81
- User Management
  85
  - User Identification
    85
  - User Classes
    85
  - Enabling Access Control
    87
  - Managing User Groups
    87
  - Change Current User
    88
  - Revert to First User
    89
- Calibration
  89
  - Calibrating Plate Positions
    90
  - Calibrating Plate Heights
    92
  - Calibrating Volumes
    97
  - Calibration Management
    100
  - Saving Calibration Data
    100
  - Reinstating Calibration Data
    102
  - Deleting Calibration Data
    103
- Run Files
  104
- Working with Run Files
  104
  - Template Protocol Run Files
    104
  - Obtaining Run Files
    105
  - Opening New or Existing Run Files
    105
  - Saving Run Files
    106
  - Creating a Run File with the "Vacuum Extraction" Wizard
    107
  - Starting the "Vacuum Extraction" Wizard
    108
  - Vacuum Extractor Configuration Wizard" Step
    108
  - Configuration [1]" Step
    111
  - Configuration [2]" Step
    112
  - Load Pre-Capture Reagent 1" Step
    113
  - Load Capture Plate" Step
    115
  - Wash Step 1 to Wash Step N" Step
    115
  - Dry Sample" Step
    117
  - Elution Steps" Step
    117
    
    Wizard Summary
    119
    
    Specifying Vacuum Processing
    120
    
    Configuring Tip Air Intake
    121
    
    Setting Run Parameters
    121
    
    Configuring Pipet Speed
    122
    
    Using Liquid-Level Tracking
    123
    
    Configuring Error Reporting
    124
    
    Setting Tip Use Options
    124
    
    Setting the Default File Location
    126
    
    Resetting to Default Values
    127
    
    Saving Current Settings as New Defaults
    127
    
    Setting System Color Configuration
    127
    
    Controlling Communication and Accessories
    130
    
    Generating Filenames for Reaction Data Export
    130
    
    Selecting a COM Port
    130
    
    Setting Shutdown Beep
    130
    
    Setting the Screen Saver Operation
    130
    
    Using the Service Register
    131
    
    Using the UV Lamp
    133
    
    Using the HEPA Filter
    134
6 Operating Procedures

135
- Switching on the Qiacube HT
  135
- Assembling the Vacuum Chamber
  135
  - For Plates from Qiacube HT Kits
    135
  - For Capture Plate VX or Capture Plate DX
    137
- Qiacube HT Protocols
  140
  - Performing a Protocol
    140
  - Pre-Run Report
    142
  - Pre-Run Check Errors and Warnings
    144
  - In-Run Open System/Open Circuit Test
    144
  - In-Run Plate Detection Test
    145
  - In-Run Interactive Vacuum Steps
    146
  - Modifying a Protocol Reaction List
    148
- Canceling a Protocol
  148
- Post-Run Report
  149
7 Maintenance Procedures

151
- Servicing
  151
- Annual Preventive Maintenance
  151
- Regular Maintenance Procedures
  151
- Weekly Maintenance Procedure
  158
- Pipetting Head Service Kit
  159
- Replacing HEPA Filters
  159
- Replacing Filters on the Vacuum Station
  160
- Shutting down the Qiacube HT
  161
8 Troubleshooting Guide

162
- Run Setup Errors
  162
- Important Error Messages
  163
  - Software Errors
    163
  - Instrument Errors
    163
  - Error Errors
    164
9 Technical Specifications

165
- Environmental Conditions
  165
  - Operating Conditions
    165
  - Transportation Conditions
    165
  - Storage Conditions
    165
- Mechanical Data and Hardware Features
  166
  - Instrument Dimensions and Weight
    166
  - Worktable Capacity
    166
  - Liquid Handling
    166
    
    Hardware and Software Features
    167
    
    HEPA Filter Unit
    167
    
    Instrument Lid
    167
  - Tip Pickup Mode
    169
  - Detect Tip Absence
    169
Appendix A Advanced Settings

169
Appendix B

189
- Declaration of Conformity
  189
- Waste Electrical and Electronic Equipment (WEEE)
  189
- FCC Declaration
  190
- Liability Clause
  190
Appendix C - Ordering Information

191
Appendix D - Informations de Sécurité

192
- Utilisation Appropriée
  192
- Sécurité Électrique
  194
- Sécurité Biologique
  195
  - Échantillons
    195
- Produits Chimiques
  195
  - Vapeurs Toxiques
    196
- Elimination des Déchets
  196
- Dangers Mécaniques
  196
- Sécurité Relative à la Maintenance
  197
- Symboles Sur le Qiacube HT
  197
Appendix E - Sicherheitshinweise

198
- Sachgemäße Handhabung
  199
- Schutz vor Stromschlag
  200
- Biologische Sicherheit
  201
  - Proben
    201
- Chemikalien
  202
  - Giftige Dämpfe
    202
- Entsorgen von Abfällen
  202
- Gefahren durch Mechanische Teile
  202
- Sicherheitshinweise - Wartungsarbeiten
  203
- Symbole auf dem Qiacube HT
  204
- Document Revision History
  205

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