Qiagen PyroMark Q96 ID User Manual page 66

PyroMark Q96 Software
18. Start the run by clicking
19. The "Run" view becomes active, and the run progress
20. When dispensation of enzyme and substrate is complete,
21. Function test: Open the instrument lid.
22. Open the process chamber lid.
23. Close the process chamber lid and the instrument lid.
24. Click on each well in "Well overview". The intensity level
25. Click "Stop" in the run progress bar. Click "Yes" in the
6-8
The flashing "Information" light will go out and the
"Busy" light will illuminate.
bar displays the current state of the run.
The chamber and heating block temperatures and
pressure will stabilize to preset values. When these values
are reached, dispensation of the enzyme (beige) and
substrate (purple) begins.
the pressure increases to the nucleotide pressure value
defined in the Instrument Parameters. The run progress
bar displays "Run", and dispensation of the nucleotides
begins.
There will be an audible warning signal and the yellow
"Information" light will illuminate.
The X-Y table should move at a rate of about 1 well
every 0.6 s; the pneumatic dispensation system should
deliver an air pulse every 0.6 s; the heating block should
oscillate (vibrate).
The "Information" light on the instrument will go out and
the audible warning signal will cease.
in the Pyrogram should be about 4000 for all wells.
dialog box that appears. The run will be stopped
immediately and the data will be saved.
PyroMark Q96 ID User Manual 01/2016
, and check that the run starts.