Qiagen QIAcube HT User Manual
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Qiagen QIAcube HT User Manual

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June 2023
QIAcube
HT
®
Version 4.17
User Manual
For QIAcube HT and QIAxtractor
®
Sample to Insight

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  • Page 1 June 2023 QIAcube ® Version 4.17 User Manual For QIAcube HT and QIAxtractor ® Sample to Insight...

  • Page 2: Table Of Contents

    Intended use of the QIAcube HT ......................11 Requirements for QIAcube HT users ..................... 12 Requirements for using dedicated QIAcube HT kits on the QIAcube HT ..........12 Requirements for using dedicated QIAcube HT kits on the QIAxtractor ..........12 QIAcube HT Software ........................
  • Page 3 Principles of operation ........................23 Vacuum chamber and components for use with QIAcube HT kits on QIAcube HT or QIAxtractor instruments Vacuum chamber and components for use with Capture Plate VX or Capture Plate DX on the QIAxtractor instrument ............................. 25 Vacuum control station and waste bottle ..................
  • Page 4 Configuring reagents ......................... 69 Required reagent volume ......................70 Samples and reactions ........................71 Sample properties ........................72 Reaction properties ........................73 Common properties and operations ..................... 76 Entering sample bar codes ......................76 QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 5 “Configuration [1]” step ......................111 “Configuration [2]” step ......................112 “Load Pre-Capture Reagent 1” step .................... 113 “Load capture plate” step ......................115 “Wash step 1 to wash step n” step .................... 115 QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 6 Operating Procedures ..........................135 Switching on the QIAcube HT ......................135 Assembling the vacuum chamber ....................... 135 For plates from QIAcube HT kits ....................135 For Capture Plate VX or Capture Plate DX ................... 137 QIAcube HT protocols ........................140 Performing a protocol ......................
  • Page 7 Pipetting head service kit ........................159 Replacing HEPA filters ........................159 Replacing filters on the vacuum station ....................160 Shutting down the QIAcube HT ......................161 Troubleshooting Guide ..........................162 Run setup errors ..........................162 Important error messages ......................... 163 Software errors ........................
  • Page 8 Sample and reaction functions for non-wizard protocols ................175 Choosing an accessory ......................... 175 Choosing the tip type ........................... 176 Working with sample banks ........................177 Configuring reactions ........................... 180 Description of the Generic XML Format ......................187 QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 9 Vapeurs toxiques ..........................196 Elimination des déchets ..........................196 Dangers mécaniques ..........................196 Sécurité relative à la maintenance ....................... 197 Symboles sur le QIAcube HT ........................197 Appendix E – Sicherheitshinweise ......................... 198 Sachgemäße Handhabung ......................... 199 Schutz vor Stromschlag ..........................200 Biologische Sicherheit ..........................

  • Page 10: Introduction

    Thank you for choosing the QIAcube HT. We are confident it will become an integral part of your laboratory. Before using the QIAcube HT, it is essential that you read this user manual carefully and pay particular attention to the safety information.

  • Page 11: General Information

    QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques.

  • Page 12: Requirements For Qiacube Ht Users

    Requirements for using dedicated QIAcube HT kits on the QIAxtractor To process dedicated QIAcube HT kits on the QIAxtractor instrument, QIAcube HT Software version 4.17 or higher is needed together with the Accessories Pack, QXT (see Appendix C – Ordering Information).

  • Page 13: Safety Information

    Safety Information Before using the QIAcube HT, it is essential that you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition.

  • Page 14: Electrical Safety

    The robotic arm moves during position calibration while the instrument lid is raised. Never click any buttons while parts of your body are within the instrument workspace. In case of emergency, switch off the QIAcube HT at the power switch at the rear of the instrument and unplug the power cord from the power outlet.

  • Page 15: Biological Safety

    If liquid has spilled inside the instrument, switch off the instrument, disconnect it from the power outlet, and contact  QIAGEN Technical Services. If the instrument becomes electrically unsafe, prevent other personnel from operating it, and contact QIAGEN Technical  Services; the instrument may be electrically unsafe when: The line power cord appears to be damaged.

  • Page 16: Chemicals

    WARNING Moving parts Avoid contact with moving parts during operation of the QIAcube HT. Under no circumstances should you place your hands under the robotic arm when it is lowering. Do not attempt to move any plates or tubes while the robotic arm is operating.

  • Page 17: Maintenance Safety

    Do not place any items on the robotic arm.  Select Pause, located in the toolbar of the QIAcube HT Software, to pause the instrument before removing plates or tubes  from the worktable. Wait until the robotic arm has completed the current movement — this may take up to 10 seconds.

  • Page 18: Symbols On The Qiacube Ht

    Type plate on the back of the RoHS mark for China (the restriction of the use of certain instrument hazardous substances in electrical and electronic equipment) Type plate on the back of the Consult instructions for use instrument QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 19: General Description

    General Description The QIAcube HT delivers high performance and reliability, enabling purification of high-quality nucleic acids from 8–96 samples per run, in increments of 8 samples. Innovative instrument features ensure reliable purification and minimize the risk of contamination. The QIAcube HT is a fully integrated solution and provides reagents, plasticware, and optimized protocols for nucleic acid purification from virtually any type of sample.

  • Page 20: Instrument Lid

    The UV lamp does not irradiate certain areas, including the pipetting head, due to construction constraints. The UV lamp is enabled and time of exposure is set within the QIAcube HT Software. For more details, see Section 5.18.3, page 133.

  • Page 21: Pipetting Head

    Tip chute Tip chute. Tips used on the worktable are discarded through the tip chute. Located at the rear-left of the QIAcube HT, the tip chute is held in place by a locating block, allowing easy placement and removal for cleaning.

  • Page 22: Accessory Panel

    Accessory panel All instrument electronics are housed in the rear of the instrument. The accessory panel, located at the rear of the QIAcube HT, provides ports for connection to the instrument. QIAcube HT accessory panel. Accessory 1 Accessory power outlet...

  • Page 23: Principles Of Operation

    Riser block EMTR Vacuum chamber, channeling adapter, and riser block EMTR (unassembled). The QIAcube HT vacuum chamber is composed of two separate compartments for waste (left) and elution (right). This configuration is one of many measures that minimize sample cross-contamination during purification.
  • Page 24 Vacuum hoses must not be modified (e.g., shortened or lengthened). Vacuum waste hose. The transfer carriage provides a platform for transporting the capture plate between the waste and elution compartments of the vacuum chamber. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 25: Instrument

    Transfer carriage for use with QIAGEN plates (e.g., QIAamp plate) other than Capture Plate VX or Capture Plate DX. Vacuum chamber and components for use with Capture Plate VX or Capture Plate DX on the QIAxtractor instrument Note: This description is for QIAxtractor instruments only. All QIAcube HT instruments are delivered with the vacuum chamber components for dedicated QIAcube HT kits.
  • Page 26 For information about installation, see Section 4.4.3, page 34. Vacuum hoses must not be modified (e.g., shortened or lengthened). Vacuum waste hose. The transfer carriage provides a platform for transporting the capture plate between the waste and elution vacuum chamber. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 27: Vacuum Control Station And Waste Bottle

    Vacuum control station and waste bottle Vacuum control station. The vacuum control station, an integral component of the QIAcube HT, provides vacuum to the vacuum chamber for processing. The vacuum is precisely controlled by the user using the QIAcube HT Software.

  • Page 28: Pinch Valve

    “full” position (at approximately 2 liters), the user will be prompted to empty the waste bottle. To empty the bottle and acknowledge the alert, use the procedure described in Section 7.2, page 151. Vacuum bottle cap. Pinch valve Pinch valve (base) and silicone hoses. Silicone hoses Pinch bar QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 29: Reagent Trough Adapters And Reagent Troughs

    Reagent trough adapters and reagent troughs The QIAcube HT is delivered with 2 reagent trough adapters for securely positioning reagent troughs on the instrument worktable: The QIAcube HT specific reagent trough adapter is always located in the R1 position of the worktable.

  • Page 30: Tip Disposal Box

    Alignment of tip disposal box holder to the instrument and the tip disposal box. Tip disposal boxes are supplied with the QIAcube HT and can be reordered from QIAGEN. (See Appendix C – Ordering Information.) QIAcube HT Version 4.17 User Manual...

  • Page 31: Qiacube Ht Laptop, Qiacube Ht Software And User Manual

    The QIAcube HT is operated via a computer. A laptop with the correct specification for operation of the QIAcube HT and QIAcube HT Software is supplied as part of the QIAcube HT System. However, if a different computer is used to operate the QIAcube HT or to run the QIAcube HT Software, then the following desktop or laptop hosting systems are recommended.

  • Page 32: Installation Procedures

     should be voltage regulated and surge protected. The QIAcube HT can be installed on any level and sturdy laboratory bench or table. There should be space below for the  vacuum control station and space adjacent for the laptop.

  • Page 33: Unpacking The Instrument

    100–240 V, 250 VA, 50–60 Hz, 5 A fuse  Make sure the voltage rating of the QIAcube HT and laptop are compatible with the AC voltage available at the installation site. Main supply voltage fluctuations must not exceed 10% of nominal supply voltages.

  • Page 34: Hardware Installation

    The male end of the 15-pin accessory cable is plugged into one of the unused accessory ports on the rear panel of the instrument (Section 3.1.6, page 22) and the female end into the accessory port on the vacuum control station. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 35: Pinch Valve

    While pulling the hose down, use the other hand to reach through the pinch valve opening and push the hoses down through the gap between the rear cover and the pinch valve. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 36 Attach the vacuum waste hose by pushing the end that does not have an attachment onto the Y-hose connector. Note: If the instrument needs to be repacked for any reason, the Y-hose connector and vacuum chamber hoses should be stowed behind the rear cover to prevent damage. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 37: Software Installation

    Software installation Note: QIAcube HT Software must be installed by a user with Windows administrator rights (see Section 4.5.1, page 37) before the instrument can be connected to the laptop for the first time. Installing the QIAcube HT Software Note: The software will usually be installed on the laptop by a QIAGEN Field Service Specialist on the day of instrument installation, if the QIAcube HT was purchased with an installation pack.
  • Page 38 6. Click Install Operating Software to begin the installation process. The “Welcome to the QIAcube HT Setup Wizard” window appears, informing the user of the software version and recommending that the user close all other programs before installing the software.
  • Page 39 If you choose to uninstall the previous version of software, existing calibration settings and run files are retained for use with the new software version. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 40 After the region is selected the Next button becomes active. 11. Click Next to continue. 12. In the “Select Additional Tasks” window, specify which additional tasks should be performed while the program is installing the software. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 41 13. Click Next to continue. 14. Review the installation parameters in the “Ready to Install” window, and click Install to continue. The release notes for the software version are displayed. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 42 15. Review the displayed release notes, and click Next to continue. 16. In the “Completing the QIAcube HT Setup Wizard” window, check Launch QIAcube HT to launch the software after the wizard closes. 17. Click Finish to complete the installation process.

  • Page 43: Connecting The Instrument To The Laptop

    The QIAcube HT uses USB for connection to the computer. The laptop which is supplied with the QIAcube HT should be installed via USB. Note: Connect the computer to the QIAcube HT either via the USB or via the RS-232 interface. Do not make both connections at the same time.

  • Page 44: Hardware Wizard

    Hardware Wizard The first time the QIAcube HT is connected to the laptop, the Windows “Found New Hardware” wizard appears. 1. Select No, not this time, and click Next. 2. Select Install the software automatically (Recommended), and click Next. QIAcube HT Version 4.17 User Manual...

  • Page 45: Configuring The Com Port

    Configuring the COM port The COM port used to connect the computer to the QIAcube HT must be correctly configured in the software to enable the software to communicate with the QIAcube HT instrument. By default, the software is configured to use COM1, and this corresponds to the first RS-232 port on the system.

  • Page 46: Important Notes Before First Use

    After configuration, the COM port selection can be changed (see Section 5.18.1, page 130). Important notes before first use The QIAcube HT has a variety of moving parts which present a number of significant pinch points. For your own safety and to prevent damage to the instrument, please observe the following: Always use the packaging provided when moving the instrument.

  • Page 47: Qiacube Ht Software

    QIAcube HT Software The QIAcube HT Software provides a user-friendly, easy-to-use interface for configuring and running protocols on the QIAcube HT instrument. When started, the software automatically detects if the instrument is switched on and connected properly. The software displays a layout that corresponds to the setup of the instrument worktable.

  • Page 48: Starting The Qiacube Ht Software

    Protocols can be specified and saved in the “Virtual” mode to create templates for subsequent runs. This feature enables the user to create protocols on any laptop that has QIAcube HT Software installed. The template can be transferred to the laptop connected to the instrument, minimizing the time required for working on the laptop connected to the instrument.
  • Page 49 Since the communication process takes several seconds, it is possible to start in “Virtual” mode by clicking Virtual Mode in the QIAcube HT window. After beginning operation in either mode, the software displays options for creating a new run or opening an existing run. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 50 (see Section 5.15.1, page 104). 5. Alternatively, click Cancel to exit the form. This creates an empty project based on the last layout used. For more information about available options, see Section 5.15.3, page 105. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 51: The Software Workspace

    When a new protocol is created using the “Vacuum extraction” wizard.  The software workspace displays positions that correspond to slots on the QIAcube HT worktable. Items specified in positions in the software workspace, referred to as “plates”, correspond to actual items placed on the worktable, and include the following: Multiwell plates (e.g., 96-well plates)

  • Page 52: Virtual" Mode Indicator

    Warnings are displayed in this bar when there is a problem with a run. For more information about the options available from the toolbar, see Section 5.5, page 58. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 53: Workspace Items

    This area provides access to all functions for setting up reagents, samples, and reactions. The contents of the right-hand pane change, depending on the item selected. For more information about working with the right-hand pane, see Section 5.6, page 60. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 54: Status Bar

    Liquid-level tracking mode; double-click to toggle  Instrument model and COM port (when instrument is connected)  QIAcube HT Software version number; double-click to review software and instrument details  Individual operations being performed and error conditions; double-click to review the diagnostic log ...

  • Page 55: Control" Menu

    Generate report Generates a pre-run report describing the required instrument setup and actions to be performed during a protocol (Ctrl+R) run. The report can be exported, printed, or saved (see Section 6.3.2, page 142). QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 56: Options" Menu

    Identifying the instrument serial number Each QIAcube HT is identified by a unique serial number, which is displayed on the serial number badge at the rear of the instrument. The serial number is also stored electronically in the instrument, allowing the instrument to be identified by the software.
  • Page 57 To view the software version number, open the Help menu from the menu bar and select About QIAcube HT…. The version number is displayed above the tabs. This software version number is also displayed in the lower left region of the status bar in the QIAcube HT Software window (Section 5.3.6, page 54).

  • Page 58: Toolbar

    Toolbar The toolbar located at the top of the QIAcube HT Software window provides easy access to the main functions in the software. Some of these functions can also be accessed from the menu bar. The toolbar. File functions Icon...
  • Page 59 Developed page 104. template Displays protocol Opens a browser window to display optional read-only protocol details (when the loaded run is a template). information For more details, see see Section 5.15.1, page 104. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 60: Right-Hand Pane

    The right-hand pane can be closed by clicking the cross at the top, right corner. The contents of the right-hand pane are described for the various workspace items. For more details, see Section 5.7, page 61. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 61: Workspace Items

    Clicking a workspace item displays information about the item in the right-hand pane. Plates A “plate” is a workspace item (Section 5.7, page 61) that corresponds to an object placed on the QIAcube HT worktable in a slot or in the vacuum chamber.

  • Page 62: Choosing The Plate Type

    1. Open the software workspace (see Section 5.3, page 51). 2. Right-click the position of the plate to be chosen. 3. From the menu that appears, select Change plate type. The “Plate Configuration” window appears. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 63: Defining Favorite Plate Types

    4. To display plates that are not tip racks, select QIAGEN and Other in the “Filter to” panel. For more information about tip racks, see Section 5.9, page 65. 5. From the “Select Plate Type” drop-down list, select type of plates to be displayed in the “Plate” list.

  • Page 64: Changing Plate Function

    5. To remove a plate from the favorites list, select a plate from the filtered favorites list and click Remove Favorite. 6. To add a new plate to the favorites list, click another “Filter to” option (such as QIAGEN or Other), choose the desired plate, and click Add Favorite.

  • Page 65: Tips

    Information about a selected tip-rack plate is displayed in the right-hand pane (Section 5.6, page 60). Right-hand pane information for a tip-rack plate. In addition to the properties that all plates have (see Section 5.8.1, page 61), tips have the properties listed in the following table. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 66: Tip Availability

    Used (dirty) tips — red (considered unavailable for software calculations)  Tip availability options are set with the tip operations menu, which is accessed by right-clicking a tip rack on the software workspace. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 67 The color of the tip positions changes color to indicate the status. 4. Repeat the process until the availability is set for all tips. The color of the tip positions changes to indicate the change in status. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 68: Reagents

    “destination” reaction wells during protocol processing. Each reagent normally performs a specific function in the process of sample purification, such as digestion, binding, or washing. In typical QIAcube HT protocols, reagents are defined in the following plate types (Section 5.8, page 61): Adapter, Reagent Trough SBS Plate ...

  • Page 69: Configuring Reagents

    Reagents can be configured by completing the following steps: 1. Open the software workspace (see Section 5.3, page 51). 2. Right-click the position of the reagent to be specified. Initially, the reagent position in the software workspace appears gray. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 70: Required Reagent Volume

    The required reagent volume includes the volume of reagent dispensed, as well as any additional volume necessary for accurate liquid transfer (specified as “Extra Vol.”; also see Section 5.10.1, page 68). The required reagent volume is displayed in the reagent position. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 71: Samples And Reactions

    Sample and reaction containers, such as lysis plates and reagent troughs, have identifiers to allow a unique description of the raw sample material to be tracked to other reaction wells (e.g., the capture plate) during liquid transfer. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 72: Sample Properties

    Information about a selected sample segment on a plate (Appendix A – Sample and reaction functions for non-wizard protocols) is displayed in the right-hand pane. Properties of sample segments are described in the following table. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 73: Reaction Properties

    Sample Bank Name A sample bank is a selection of wells in a plate segment that is grouped and treated as a single unit for liquid-handling operations. In the QIAcube HT Software, sample banks are always aligned to the 8- channel pipettor.
  • Page 74 The concentration shown is either the value explicitly assigned for the well, or a value calculated based on the concentration of sample or reaction contents transferred from other plates or reagent troughs. Bar code See “ID” entry above. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 75 Highlights sample banks that are involved in a selected reaction in orange. Click the icon again to remove the highlight. Highlights destination reaction wells that are involved in a selected reaction in blue. Click the icon again to remove the highlight. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 76: Common Properties And Operations

    These simulated tabs or carriage returns automatically move the data input point (cursor) on the data table to the next input cell each time a bar code is read. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 77: Data Transfer Formats

    In the export mode where the data can be defined by the user, the user must specify the data content by writing an export string. The following table lists the data designators for export strings. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 78: Importing Sample And Reaction Data

    Sample names can be easily copied from a spreadsheet by copying the column that contains the sample name information. The copied entries are pasted to the QIAcube HT Software by right-clicking a cell and selecting the “Paste to current cell” item from the menu that appears.
  • Page 79 During the import, the wells to which data are imported are added to the selected sample bank. Existing members of the sample bank prior to import are retained unchanged. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 80 Note: Multiple sample banks are simultaneously imported using the Banks named from membership columns function. Alternate strategies allow multiple sample banks to be successively populated from the same import file. For example, the QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 81: Exporting Sample And Reaction Data

    Sample and reaction data, which is listed in the sample and reaction right-hand pane, can be exported in a number of formats. This can be used to transfer data to the QIAGEN QIAgility or Rotor-Gene Q instruments, or other data storage systems (e.g., ®...
  • Page 82 A *.csv file with format fixed as “well-location, plate identifier, sample identifier” (using the substitution pattern %2%0%P%0%I, as described in Section 5.11.5, page 77). This describes the mapping of a tube or well location (plate and well label) to its contents (sample identification). QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 83 Uses the initial concentration value. This is associated with the source sample or standard well. Final Reaction Uses the final concentration value of the sample or standard after dilution with other liquids. This is associated with the reaction well. Page Option QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 84 The index of the well in the (horizontal) sequenced grid export is used as the identifier. 5. Click Export. 6. Enter a file name and select the location to which the data will be exported. 7. Click Save to continue. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 85: User Management

    User management User management allows the definition of different access rights to various data and functions within the QIAcube HT Software. User management functions are accessed from the menu bar (Options > User Management). User identification Unlike software that requires separate login authentication, the QIAcube HT Software user is defined as the current Windows user logged on the laptop on which the software is installed.
  • Page 86 Upon restarting the software, only Administrators and users assigned to the “Manager”, “Designer” and “Operator” groups can operate the software. When attempting to start the QIAcube HT Software, users that are not assigned to a user groups are denied access to the software.

  • Page 87: Enabling Access Control

    Enabling access control Upon installation, the QIAcube HT Software user access control features are disabled and all users have “Manager” access (see the previous section for user group descriptions). A Manager enables the user access control with Options > User management > Enable access control.

  • Page 88: Change Current User

    Alternatively, select Options > User management > Change Current User. The “Change User” window appears. The new user must enter their username and password, and click OK to log on. If the login was successful the new rights will take effect immediately. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 89: Revert To First User

    The use of a joy pad (a game controller that has only buttons and no stick) can make position calibration significantly easier. A USB joy pad can be connected to the laptop that controls the QIAcube HT. During calibration, the user controls the robotic arm movement with the joy pad.

  • Page 90: Calibrating Plate Positions

    Calibrating plate positions Plate position calibration defines the positions of wells or tubes in a plate to be used on the QIAcube HT worktable. Calibration ensures that the robotic arm moves to the correct location for every well on the plate.
  • Page 91 9. Lower the pipetting head by clicking Lower Tip several times until the tip (or pipettor hub if no tips are attached) is just above (approximately 2–5 mm), but not touching, the plate. Make sure the tip (or hub) is not touching the plate or consumable loaded in the plate before proceeding. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 92: Calibrating Plate Heights

    Calibrating plate heights Plate height calibration defines the base position of the wells of a plate to be used on the QIAcube HT worktable. Calibration allows software to correctly calculate the height at which tips must be placed for optimal aspirate and eject pipetting operations.
  • Page 93 “supernatant” sample or lysis plates are used, manual height calibration is necessary and the height must be set such that tips touch the surface of a 200 µL liquid volume preloaded into the plate (i.e., approximately 5 mm above the true base of the plate). QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 94 Note: Height calibration data for QIAGEN capture plates are available within the QIAcube HT Software and normally do not require adjustment by the user. However, if height calibration is required, only the manual calibration method can be used as the silica membrane located at the base of each well cannot tolerate contact (which would occur using automatic height calibration).
  • Page 95 5. Select Options > Robot Setup > Calibrate plate height. 6. Read the warning about using the height calibration function, and select Yes. The “Height Calibration” window appears (see figure in the previous section). QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 96 (approximately 1 mm), but not touching, the plate. Make sure the tip is not touching the plate or consumable loaded in the plate before proceeding. This step ensures greater accuracy. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 97: Calibrating Volumes

    “Option 1” from the “Volume calibration” wizard. Before starting the first run on a newly installed QIAcube HT volume calibration has to be done. If no volume calibration has been done an error is shown at start of a run.
  • Page 98 Option 1 Option 1 allows manual adjustment of the P-axis unit value for specific volumes. This method is used to enter the values supplied in the volume calibration report provided by QIAGEN. 1. Select Option 1 from the “Volume calibration” wizard.
  • Page 99 9. Click Close to close the report and return to the wizard. For more information about the report options, see Section 6.3.2, page 142. 10. Click Finish to close the wizard and return to the main software window. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 100: Calibration Management

    The setup disk is used to transfer instrument-specific data when the instrument is connected to a new laptop. It is not necessary to use a setup disk when the software is updated to a new version. Note: QIAcube HT run files (e.g., “runfile.QSP”) are not instrument specific and can be transferred between laptops connected to different instruments.
  • Page 101 A default file name and location is supplied. It is possible to browse to the folder where the setup disk should be stored. 4. Click Save to create the setup disk as specified. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 102: Reinstating Calibration Data

    5. If the identifier in the setup disk matches the connected instrument, a dialog box appears prompting to restart the software. Click Yes (recommended). 6. If necessary, indicate whether the current run file should be saved. The calibration data will be reinstated when the QIAcube HT Software is restarted. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 103: Deleting Calibration Data

    1. From the Options menu in the menu bar, select Calibration Management > Delete multiple calibrations …. The “Delete Multiple Calibrations” dialog box appears. 2. Select the type of data to be deleted. 3. Delete the data by clicking Delete. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 104: Run Files

    Run files QIAcube HT run files (file extension *.QSP) contain all the information required to perform a protocol on the QIAcube HT instrument. The run file specifies protocol settings and instrument-control parameters, including: Liquid transfer  Air volumes  Pipetting speeds ...

  • Page 105: Obtaining Run Files

    When a template run is explicitly unlocked, any optional read-only protocol information initially provided by QIAGEN in the original template is removed from the run, because the structure of the protocol can be changed and may diverge from the original.

  • Page 106: Saving Run Files

    This notification also appears when an attempt is made to exit the software (e.g., using the File > Exit menu item or clicking the cross in the title bar) while working with a modified run file. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 107: Creating A Run File With The "Vacuum Extraction" Wizard

    On successful completion of the wizard using the Finish button of the wizard summary page, the software compiles the protocol configuration and updates the list of operations displayed in the reaction list in the right-hand pane. All reaction steps existing prior to opening the wizard are discarded. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 108: Starting The "Vacuum Extraction" Wizard

    The sample plate is manually placed on the worktable and the supernatants or samples are subsequently transferred automatically to the lysis plate. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 109 5. Select Wet capture plate before loading sample? to include a step that wets the capture plate before the lysed samples are loaded. This step is not usually required for standard nucleic acid purification. 6. Click Next to continue. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 110 Note: Plate type must be correctly specified before its function can be determined. 5. Specify the plate function by completing the window that appears as described in Section 5.8.4, page 64. 6. Click Next to continue. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 111: Configuration [1]" Step

    5. Select columns to be used for processing by clicking. Alternatively, click the Select All to select all columns. Selection can be cleared by clicking Clear All. 6. Click Next to continue. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 112: Configuration [2]" Step

    3. Enter reagent details. For more details, see Section 5.10.1, page 68. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 113: Load Pre-Capture Reagent 1" Step

    Information in the “Load lysis” panel reflects the selections made in the “Vacuum Extractor Configuration Wizard” window (Section 5.16.2, page 108). 2. Select the first lysis reagent and volume to be processed. 3. Select the sample volume to be transferred. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 114 If the working volume is exceeded, a notification appears requiring the volumes to be adjusted accordingly before the protocol configuration can be finalized. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 115: Load Capture Plate" Step

    (post capture)?” field of the “Vacuum extractor configuration wizard” step (Section 5.16.2, page 108). A similar window is shown for each wash reagent. Consecutive applications of the same wash reagent (iterations) are configured in the step for the wash. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 116 3. If the wash should be incubated at room temperature (15–25°C) on the capture plate before continuing, enter the incubation time. 4. Specify vacuum processing. For details, see Section 5.16.12, page 120. 5. Select the number of times the wash reagent should be applied (iterations). 6. Click Next to continue. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 117: Dry Sample" Step

    3. Click Next to continue. “Elution steps” step Before the elution steps are processed, the QIAcube HT moves the transfer carriage with the capture plate from the waste side of the vacuum chamber to the elution side of the vacuum chamber.
  • Page 118 In the second elution step, TopElute fluid is added to the elution buffer then vacuum is applied. TopElute fluid only partially passes the membrane thereby preventing the flow of air through empty wells. 8. Click Next to continue. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 119: Wizard Summary

    Print: Print the report  Save: Save the report in HTML format  Email: Save the report as an e-mail attachment  MSWord: Save the report as a Microsoft Word ® document  QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 120: Specifying Vacuum Processing

    Excessive vacuum may cause aerosol from the capture plate membrane, collapse of the capture plate membrane, drying of salts and detergents onto the membrane, and foaming. Insufficient vacuum may cause reduced performance and, when drying membranes, require longer drying times. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 121: Setting Run Parameters

    The air is subsequently blown out after liquid ejection to clear the tip. Higher air volumes may assist when pipetting viscous liquids. Air volumes are set separately for different classes of liquids (e.g., samples and reagents). We recommend 0 µL as the air intake volume for QIAcube HT applications. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 122: Configuring Pipet Speed

    Slower speeds can be chosen for pipetting viscous liquids. When specifying reagent troughs for a layout, the reagent can be defined as “normal” or “viscous” (see Section 5.9, page 65). Settings can be returned to default values with the Options > Run Settings > Reset settings to factory defaults option. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 123: Using Liquid-Level Tracking

    Default. Software tracks liquid transfer and calculates volumes present in plates or reagent troughs, allowing tips to be placed near the estimated liquid surface to minimize contact during aspirating and dispensing tasks. Note: Use default “Use liquid level estimate” setting unless advised by QIAGEN Technical Services. QIAcube HT Version 4.17 User Manual...

  • Page 124: Configuring Error Reporting

    For runs compiled using the “Vacuum extraction” wizard: Programmed tip usage (for NA Extraction)  This option ensures no cross-contamination can occur during reagent addition and sample transfer, but reduces costs by re-using tips during intra-sample mixing operations. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 125 Important: When using this option, ensure that plates containing liquid are assigned a non-zero preload volume (right- click the plate to select and set this value, see Section 5.10.2, page 69), otherwise contamination may result. 3. Click OK to apply the settings and close the dialog box. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 126: Setting The Default File Location

    3. Click OK to apply the settings and close the dialog box. If required, subdirectories will be generated automatically in the new file location. No changes are made to the original default file location and all files are retained. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 127: Resetting To Default Values

    The “System color configuration” option allows system color configuration to be modified according to preference. From the Options menu in the menu bar, select Run Settings > System color configuration. The “Sample Color Configuration” dialog box appears. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 128 Delete selected colors. Reset all sample colors; click Yes to continue. Refresh the colors displayed on the software worktable according to the current settings. Open color theme. Save color theme. Apply changes and close. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 129 Open the Liquid Colors tab, and select colors used for reagents and other liquids. Open the Selection Colors tab and select colors used for liquid functions and for representation of members of a sample bank, as well as for selection and highlighting. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 130: Setting Shutdown Beep

    “Virtual” mode (see Section 5.1.2, page 47). Change the COM port manually as follows: 1. Run the QIAcube HT Software in the “Virtual” mode. 2. From the Options menu in the menu bar, select “Robot Setup/COM port”.

  • Page 131: Using The Service Register

    Component to be serviced. Due Date Date service is required. Acknowledged By Name of the user completing the last service acknowledgement. Acknowledge Date Date and time the user completing the last service acknowledgement. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 132 Note: Volume calibration must be done before starting the first run after acknowledging a pipettor service. If no volume calibration has been done, an error is shown at the start of a run. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 133: Using The Uv Lamp

    Note: If the UV lamp icon is grayed out in the toolbar, the UV lamp is not available. In this case check whether the UV tubular lamp is installed and if it is working correctly. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 134: Using The Hepa Filter

    The HEPA filter is turned on and off with options in toolbar. For more information about the HEPA filter see Section 4.4.2, page 34. Turn the HEPA filter on or off as follows: 1. Ensure the HEPA filter is connected to the QIAcube HT with the accessory cable before switching on the instrument and initializing the software. 2. Click in the toolbar to toggle the HEPA fan on and off.

  • Page 135: Operating Procedures

    For more details, see Section 4, page 32. Switch on the QIAcube HT follows: 1. Switch on the QIAcube HT using the power switch located at the rear of the instrument on the lower left panel. Wait until the instrument is initialized before proceeding.
  • Page 136 (cat. no. 9022655 Vacuum chamber For more information about available kits and protocols for the QIAcube HT, please visit www.qiagen.com/QIAcubeHT, or contact QIAGEN Technical Services. 1. Insert the channeling block holder into the left (waste) chamber of the vacuum chamber.

  • Page 137: For Capture Plate Vx Or Capture Plate Dx

    8. Load an elution microtubes rack (EMTR) into the elution chamber. For Capture Plate VX or Capture Plate DX Note: This description is for QIAxtractor instruments only. All QIAcube HT instruments are delivered with the vacuum chamber components for dedicated QIAcube HT kits.
  • Page 138 8. Invert the assembly. 9. Insert the assembly into the left (waste) compartment of the vacuum chamber, aligning the spigot of the mat support assembly to the drain of the chamber. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 139 15. Ensure the elution riser block is in the right (elution) side of the vacuum chamber. 16. Load a covered elution plate into the elution chamber. Squeeze the locking tabs on the elution plate lid and remove the lid. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 140: Qiacube Ht Protocols

    Protocols are a complete set of processing instructions defined in a run file. For more information about run files, see Section 5.14, page 104. To process dedicated QIAcube HT kits on the QIAxtractor instrument, QIAcube HT Software version 4.17.1 or higher is needed together with the Accessories Pack, QXT (see Appendix C – Ordering Information).
  • Page 141 3. Ensure the UV lamp is switched off. For more information, see Section 5.18, page 130. 4. Launch the QIAcube HT Software. Ensure that the QIAcube HT software launches in “Normal” mode. For more information, see Section 6.1, page 135.

  • Page 142: Pre-Run Report

    When performing a protocol, a pre-run report can be prepared from the pre-run check (see Section 6.3.1, page 140) from the Wizards/Generate Report... menu item, or by using the Generate pre-run report… toolbar item. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 143 The following options are available for the report. Print: Print  Save: Save in HTML format  Email: Save as an e-mail attachment  MSWord: Save as a Microsoft Word document  Close the pre-run report by clicking Close. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 144: Pre-Run Check Errors And Warnings

    If the achieved pressure is too low, this implies a leak, perhaps due to poor connections or incorrect fitting of the bottle cap. When the Open Circuit test fails, a warning dialog box appears. This provides more hints about how to fix the problem. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 145: In-Run Plate Detection Test

    Note: If using DX or VX reagents and plasticware, avoid pressing the capture plate nozzles down into the silicone mat holes as this may bind the capture plate and mat together and interfere with later transfer of the capture plate to the elution side of the chamber. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 146: In-Run Interactive Vacuum Steps

    Mute button. The total elapsed time is shown next to the text “Vacuum turned off since”. If Continue is selected, a new window appears with the choice either to resume the vacuum or advance to proceed with the protocol. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 147 If Resume is selected, the vacuum turns on until the Continue button is clicked. If Continue is selected, the following dialog box opens. Click Proceed to proceed with the vacuum, or click Advance to finish the vacuum step and continue with the protocol. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 148: Modifying A Protocol Reaction List

    The user is presented with options to continue or cancel the run. Selecting Cancel run causes the robot to stop all movements and terminate the run. Selecting Continue allows the run to proceed. Note: Once canceled, a run cannot be restarted from the canceled step. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 149: Post-Run Report

    Actual run duration (start and finish times)  Details of sample identifiers eluted into the elution plate (if available)  Summary of processing steps in the protocol  Selected run settings, including air volumes and pipetting speeds  QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 150 MSWord: Save as a Microsoft Word document  Close the pre-run report by clicking Close. The post-run report is automatically saved in the Reports subdirectory of the Data directory (default location is C:\Program Files\QIAcubeHT\Data). QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 151: Maintenance Procedures

    Annual preventive maintenance QIAGEN recommends that an annual preventive maintenance service is carried out on the QIAcube HT. The service should be performed by a trained QIAGEN Field Service Specialist. Contact your local QIAGEN Field Service Specialist or your local distributor for more information.
  • Page 152 1. Disconnect the waste bottle and discard the waste according to local safety regulations. 2. Replace and reconnect the waste bottle. 3. Click Bottle Emptied in the window that appears. This allows the run to proceed. Alternatively, use the Cancel button to terminate the run. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 153: Cleaning The Qiacube Ht

    Cleaning the QIAcube HT CAUTION Damage to the instrument Do not use solvents, or reagents containing acids, alkalis, or abrasives to clean the QIAcube HT. CAUTION Damage to the QIAcube HT lid Do not use alcohol, solvents, reagents containing acids, alkalis, or abrasives to clean the instrument lid.

  • Page 154: Cleaning The Instrument After Completing A Run

     should be used only for items that have been removed from the instrument. If solvents or saline, acidic, or alkaline solutions are spilt on the QIAcube HT or if QIAGEN buffers splash the instrument lid,  wipe the spilt liquid away immediately.

  • Page 155: Cleaning The Instrument After Shutting Down

    6. Remove the carriage and discard the capture plate. Clean carriage according to instructions in Section 7.2.5, page 156. 7. If using plates from dedicated QIAcube HT kits, leave the channeling adapter in position until the waste chamber has been flushed.

  • Page 156: Cleaning Instrument Worktable Accessories

    Greasing the O-ring If using plates from dedicated QIAcube HT kits, apply a thin film of vacuum grease to the O-ring, as required, on the channeling block holder indicated with a red arrow in the following figure). Channeling block holder O-ring.
  • Page 157 Waste sink/mat support O-ring. Cleaning air bleed holes The QIAcube HT and QIAxtractor instruments have air bleed holes positioned at strategic points on the vacuum chamber and the waste sink/mat support, as shown in the following figures. Each of these air bleeds has a small stainless steel aperture inserted in the hole to specifically control the rate of air flow.

  • Page 158: Weekly Maintenance Procedure

    Greasing the pipettor The pipetting head occasionally requires servicing. The software has a built-in maintenance counter that reminds the user after 300,000 pipetting operations that the pipetting head requires service. Contact your QIAGEN Field Service Specialist for servicing requirements. Cleaning the guide rails The X, Y, and Z guide rails are the coated hardened steel rails that support the linear bearings that allow the robotic arm to slide back and forth easily.

  • Page 159: Pipetting Head Service Kit

    3 or more plates per day. Replacing HEPA filters HEPA filters should be replaced on a regular basis. The QIAcube HT Software reminds you to replace the HEPA filters at 6 monthly intervals; replacement should take place then. Replace the HEPA filters as follows: QIAcube HT Version 4.17 User Manual...

  • Page 160: Replacing Filters On The Vacuum Station

    For waste disposal of the used HEPA filters follow instructions of your local regulations. Replacing filters on the vacuum station Vacuum filters should be replaced on a regular basis. The QIAcube HT Software reminds you to replace these filters at 6 monthly intervals; replacement should take place then.

  • Page 161: Shutting Down The Qiacube Ht

    Shutting down the QIAcube HT The QIAcube HT Software can be shut down by clicking in the top right hand corner of the QIAcube HT window or by selecting “Exit” in the “File” menu. During shut down, the user is prompted to clean the instrument. For more details, see Section 7.2, page 151.

  • Page 162: Troubleshooting Guide

    Troubleshooting Guide If you need to contact QIAGEN Technical Services about an error, note down the steps leading to the error, save or choose the applicable support package from the problem run, and then send this to QIAGEN Technical Services. If the support package cannot be saved because of a communication problem, start the software with the instrument powered down, save the support package immediately, and then contact QIAGEN Technical Services.

  • Page 163: Important Error Messages

    Instrument error numbers begin with 40000. Specific error codes from the instrument are reported as the sum of this base value (40000) and the code listed in the following table. The following table lists selected instrument errors. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 164: Error Errors

    An invalid COM port was chosen Choose the correct COM port An attempt was made to move an axis beyond its limit Send the robot to its home position and retry. Contact QIAGEN Technical Services. Too many replies were specified in transmitted try power off/on and restart. If continues, then contact TS packet.

  • Page 165: Technical Specifications

    Relative humidity Max. 75% (noncondensing) Storage conditions Air temperature 15°C to 30°C (59°F to 86°F) in manufacturer’s packaging Relative humidity Max. 75% (noncondensing) * Conditions under which equipment is electrically safe to operate. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 166: Mechanical Data And Hardware Features

    Depth: 400 mm (16 in.) Weight QIAcube HT instrument: 44 kg (97 lb) QIAcube HT vacuum control station: 17 kg (38 lb.) Worktable capacity The worktable provides the following positions: 4 SBS-standard plates (footprint 128 x 86 mm), compatible to accept 96-well plates or tip racks ...

  • Page 167: Instrument Lid

    Preinstalled HEPA filter unit Hardware and software features Laptop and The QIAcube HT is operated with a connected laptop. A laptop appropriate for the correct operation software of the QIAcube HT Software is supplied as part of the QIAcube HT system.
  • Page 168 Double-click the Add or Remove Programs icon, and wait for the screen to be populated.  Select QIAcube HT (Driver Removal), and click Remove to remove the USB driver.  Note: Uninstallation does not delete the installation folder (typically C:\Program Files\QIAcubeHT), allowing calibration settings, run settings, and run files to be retained.

  • Page 169: Advanced Settings

    Appendix A Advanced settings Advanced run settings are available to adjust specific functions on the QIAcube HT. These functions should be modified only under direct instruction from QIAGEN. Tip pickup mode This defines the strategy used for collecting tips from the Tip Rack Holder. Normal (single stage) or Three Stage can be selected.

  • Page 170: Compensate For Liquid Loss (Reagents)

    A tick is displayed when this function is activated. By default, all of the liquid handling functions available via the Special button, presented in the “Reaction” right-hand pane, work only with volumes up to the largest capacity tip supported on the QIAcube HT (i.e., 200 µL).

  • Page 171: Allow Preserved Well Indices

    This position is expressed as a percentage of the distance from the top of the tube to the surface of the liquid. The allowable range is 0 to 100 (%). The default value is 50. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 172: Set Anti-Drip Exhale Tip Position

    To assist with reagent drip control, these droplets are inhaled into the tips prior to the next movement of the robot arm. This function defines the time (in milliseconds) allowed for this droplet formation pause. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 173: Use Slow Move Speeds For Reagent (Post Eject)

    This aids with sample drip control to reduce cross contamination. The recommended range of values is 10 to 100 (%). For viscous liquids a value in the range 30 to 60 is suggested. The default value is 100. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 174: Set Sample Post Aspirate Pause (Msec)

    (calculated) values. In addition, this function can be used to address a variety of situations where the tip would otherwise be placed too high in non-reagent wells for pipetting operations. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 175: Use Slow Move Speeds For Sample

    When using the plate with an accessory on the worktable, the correct accessory configuration must be specified in the software workspace to ensure the correct height calibration data are used during pipetting operations. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 176: Choosing The Tip Type

    1. Open the software workspace (see Section 5.3, page 51). 2. Right-click the plate that is to be changed. 3. From the menu that appears, select Change plate type. The “Plate Configuration” window appears. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 177: Working With Sample Banks

    8. When the correct tips have been chosen, click OK to apply the changes and close the window. The selected pipet tips appear in the workspace. Note: Correct selection of tip type is critical for successful operation of the QIAcube HT. Note: Only use pipet tips recommended by QIAGEN.
  • Page 178 Enables the user to enter the volume of liquids which have been preloaded into reaction tubes. This option is only available when a reaction plate is selected. Click OK to continue. “Update sample bank” window. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 179 Cancel Close the “Update Sample Bank” window. More Open the “Sample selection” and “Filter selection” panels, in addition to the “Sample Bank Name” panel. Less Close the “Sample selection” and “Filter selection” panels. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 180: Configuring Reactions

    Reaction configuration allows the user to access options for combining liquids or performing other instrument functions as part of the run protocol. These facilities extend the use of QIAcube HT beyond its primary purpose as a wizard-driven nucleic acid purification system.
  • Page 181 1. Open the software workspace (see Section 5.3, page 51). 2. Right-click the reaction plate to configure. Reaction plate information is displayed in the right-hand pane. 3. Click Add... in right-hand pane. The “Reaction Configuration” window appears. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 182 The volume mixed is the larger of either the well contents or the last volume of liquid added. This option is particularly useful when pipetting a very small sample volume (<3 µL). Mixing ensures the entire sample is “rinsed” out of the tip. 7. To apply the changes and close the window, click OK. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 183 This function instructs the instrument to start pipetting into a reaction plate from its first well, typically well A1. No additional configuration is needed. Special operation: Add “pause until confirmation” This function displays an optional user-defined message and pauses the instrument until a specified user action is performed and OK is clicked. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 184 The Ignore pressure feedback? option allows the next operation to proceed without first confirming that the vacuum has reached the target vacuum pressure. Note: Only an experienced user should use the latter two functions. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 185 8 samples is replicated 5 times using standard “general liquid handling” reaction steps (i.e., prepared using Add…), 40 tips are required. When the Add sample bank duplication step… option is used, only 8 tips are required for this operation. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 186 Defines the sample volume that will be used for replication. Click Add to include this target configuration to the replicate list. Select a replicate from the list and click Remove to delete this replicate from the duplication series. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 187: Description Of The Generic Xml Format

    <PlateID/> <PlateType>Std96</PlateType> <Size>96</Size> <Wells> <Well> <Label>A1</Label> <WellID/> <WellNo>1</WellNo> <StandardWellName>A:1</StandardWellName> <Color>5775359</Color> <TubeType>BioSprint_250</TubeType> <Concentration>37</Concentration> <FinalWellVol>80</FinalWellVol> <EventOccurred>0</EventOccurred> <Contents> <Diluent> <SocketName>M1</SocketName> <WellLabel>A</WellLabel> <Vol>40</Vol> </Diluent> <Mix> <SocketName>M1</SocketName> <WellLabel>B</WellLabel> <Vol>10</Vol> <Name>Mix B</Name> </Mix> <Reagent> <SocketName>R1</SocketName> <WellLabel>I</WellLabel> <Vol>2.5</Vol> <Name>10X Buffer</Name> QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 188 SL_FullID = 0 SL_SampleID = 1 SL_ReactionID = 2 SL_SampleName = 3 EmptyLabelType Possible values:  EL_Empty = 0 EL_WellID = 1 EL_WellOrder = 2 EL_WellNumber = 3 They can be evaluated during the transformation. QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 189: Declaration Of Conformity

    Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE- marked electronic equipment is provided.

  • Page 190: Fcc Declaration

    Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.

  • Page 191: Appendix C - Ordering Information

    Box of 10; waste collection box to fit 990550 tip ejector chute For up-to-date licensing and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.

  • Page 192: Appendix D - Informations De Sécurité

    Appendix D – Informations de sécurité Avant d'utiliser le QIAcube HT, il est impératif de lire attentivement ce manuel et de porter une attention particulière aux informations de sécurité. Afin de garantir un fonctionnement de l'appareil en toute sécurité et de maintenir l'appareil en bon état de marche, il est impératif de suivre les instructions et les informations de sécurité...
  • Page 193 Ne jamais cliquer sur une des touches tant que des parties du corps se trouvent dans l'enceinte de l'appareil. En cas d’urgence, éteindre le QIAcube HT à l’aide de l’interrupteur d’alimentation situé à l’arrière de l’appareil et débrancher le cordon d'alimentation de la prise de courant.

  • Page 194: Sécurité Électrique

    Ce dispositif ne doit en aucun cas être désactivé. Afin que le QIAcube HT fonctionne de manière satisfaisante et en toute sécurité, suivre les conseils suivants : Le cordon d'alimentation de l'appareil doit être branché sur une prise d'alimentation munie d’un conducteur de protection ...

  • Page 195: Sécurité Biologique

    ACGIH : American Conference of Government Industrial Hygienists (États-Unis d’Amérique) (Conférence américaine des hygiénistes industriels † gouvernementaux). COSHH : Control of Substances Hazardous to Health (Royaume-Uni) (Contrôle des substances dangereuses pour la santé). ‡ QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 196: Vapeurs Toxiques

    Avant de retirer des plaques ou des tubes de la table de travail, sélectionner « Pause » dans la barre d'outils du logiciel  QIAcube HT pour mettre l'appareil en mode de pause. Attendre l'arrêt complet du bras robotisé. Cela peut prendre jusqu'à 10 s.

  • Page 197: Sécurité Relative À La Maintenance

    Sécurité relative à la maintenance Procéder à la maintenance comme décrit à la Section 7. QIAGEN facture les réparations rendues nécessaires suite à une maintenance inadaptée. AVERTISSEMENT Risque d’accident corporel et de détérioration du matériel Effectuer uniquement la maintenance spécifiquement décrite dans ce manuel.

  • Page 198: Appendix E - Sicherheitshinweise

    Lire le manuel d’utilisation. Appendix E – Sicherheitshinweise Lesen Sie dieses Handbuch sorgfältig durch, bevor Sie den QIAcube HT benutzen. Beachten Sie dabei insbesondere die Sicherheitshinweise. Die Gebrauchsanweisungen und Sicherheitshinweise im Handbuch müssen befolgt werden, um einen sicheren Betrieb des Geräts zu gewährleisten und das Gerät in einem sicheren Zustand zu erhalten.

  • Page 199: Sachgemäße Handhabung

    WARNUNG/ Verletzungsgefahr und Beschädigung des Geräts ACHTUNG Der QIAcube HT ist zu schwer, um von einer einzelnen Person gehoben zu werden. Zur Vermeidung einer Verletzung oder Beschädigung des Gerätes heben sie das Gerät nicht alleine an. WARNUNG/ Verletzungsgefahr und Beschädigung des Geräts ACHTUNG Der QIAcube HT darf auf keinen Fall während des Betriebs fortbewegt werden.

  • Page 200: Schutz Vor Stromschlag

    Sie nicht auf die Maus und drücken Sie nicht auf eine Taste der Tastatur, während sich Ihre Hände oder andere Körperteile im Arbeitsbereich des Roboterarms befinden. Schalten Sie im Notfall den QIAcube HT aus (der Netzschalter befindet sich auf der Geräterückseite), und ziehen Sie den Netzstecker aus der Steckdose.

  • Page 201: Biologische Sicherheit

    Falls Flüssigkeit auf dem Gerät verschüttet wird und in das Gerät läuft, dann schalten Sie es sofort aus, trennen Sie es von  der Netzspannung (Stecker ziehen!) und setzen Sie sich mit dem Technischen Service von QIAGEN in Verbindung. Falls die elektrische Sicherheit bei der Bedienung des Geräts nicht mehr gewährleistet werden kann, muss das Gerät gegen ...

  • Page 202: Chemikalien

    Benutzte Kunststoff-Laborartikel können gefährliche Chemikalien enthalten. Derartige Abfälle müssen gemäß den geltenden regionalen Sicherheitsbestimmungen gesammelt und entsorgt werden. Beachten Sie bei der Entsorgung des QIAcube HT die gesetzlichen Bestimmungen zur Entsorgung von Elektro- und Elektronik- Altgeräten (siehe Anhang A). Gefahren durch mechanische Teile WARNUNG Sich bewegende Geräteteile...

  • Page 203: Sicherheitshinweise - Wartungsarbeiten

    Stellen Sie keine Gegenstände auf den Roboterarm.  Wählen Sie die Option “Pause” in der Werkzeugleiste der QIAcube HT Software, um das Gerät anzuhalten, bevor Sie  Reaktionsgefäße oder Platten von der Arbeitsplattform entfernen. Warten Sie, bis der Roboterarm die gerade ablaufende Bewegung beendet hat –...

  • Page 204: Symbole Auf Dem Qiacube Ht

    Typenschild auf der Geräterückseite Symbol der CSA-Zertifizierung in Kanada und den USA Typenschild auf der Geräterückseite Hersteller i.S.d. Gesetzes Typenschild auf der Geräterückseite WEEE-Kennzeichnung (Zertifizierung gemäß europäischer Richtlinien bzw. Elektro- und Elektronik-Altgeräte- Verordnung) QIAcube HT Version 4.17 User Manual 06/2023...

  • Page 205: Document Revision History

    China (Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro- und Elektronikgeräten) Typenschild auf der Geräterückseite Lesen Sie die Gebrauchshinweise im Handbuch Document Revision History Revision Description June 2023 Included the UKCA symbol. Removed the environmental class. QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 206 , Lysetol ® (Schülke & Mayr GmbH); MagNA Pure ® , Roche ® (Hoffman-La Roche, Inc.); Teflon ® (E.I. du Pont de Nemours and Company). June-2023 HB-1569-004 © 2023 QIAGEN, all rights reserved QIAcube HT Version 4.17 User Manual 06/2023...
  • Page 207 Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com QIAcube HT Version 4.17 User Manual 06/2023...

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