Sign In
Upload
Download
Table of Contents
Contents
Add to my manuals
Delete from my manuals
Share
URL of this page:
HTML Link:
Bookmark this page
Add
Manual will be automatically added to "My Manuals"
Print this page
×
Bookmark added
×
Added to my manuals
Manuals
Brands
Qiagen Manuals
Laboratory Equipment
QIAcube HT
User manual
Qiagen QIAcube HT User Manual
Hide thumbs
Also See for QIAcube HT
:
User manual
(311 pages)
,
User manual
(282 pages)
1
Table Of Contents
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
page
of
207
Go
/
207
Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
Table of Contents
1 Introduction
About this User Manual
General Information
Technical Assistance
Policy Statement
Intended Use of the Qiacube HT
Requirements for Qiacube HT Users
Requirements for Using Dedicated Qiacube HT Kits on the Qiacube HT
Requirements for Using Dedicated Qiacube HT Kits on the Qiaxtractor
Qiacube HT Software
2 Safety Information
Proper Use
Electrical Safety
Biological Safety
Samples
Chemicals
Toxic Fumes
Waste Disposal
Mechanical Hazards
Maintenance Safety
Symbols on the Qiacube HT
3 General Description
The Qiacube HT
Instrument Lid
UV Lamp
Pipetting Head
Tip Chute
HEPA Filter
Accessory Panel
Feet
Worktable
Principles of Operation
Instrument
Vacuum Control Station and Waste Bottle
Pinch Valve
Reagent Trough Adapters and Reagent Troughs
Tip Disposal Box
Qiacube HT Laptop, Qiacube HT Software and User Manual
4 Installation Procedures
Instrument Delivery and Installation
Requirements
Site
Unpacking the Instrument
AC Power Connection
Hardware Installation
HEPA Filter System
UV Lamp
Vacuum Control Station
Pinch Valve
Vacuum Waste Hose
Software Installation
Installing the Qiacube HT Software
Connecting the Instrument to the Laptop
Connecting the Computer to the Qiacube HT
Hardware Wizard
Configuring the COM Port
Important Notes before First Use
5 Qiacube HT Software
Mode
Normal" Mode
Virtual" Mode
Starting the Qiacube HT Software
The Software Workspace
Virtual" Mode Indicator
Menu Bar
Toolbar
Workspace Items
Right-Hand Pane
Status Bar
Menus
File" Menu
Control" Menu
Wizards" Menu
Options" Menu
Help" Menu
Toolbar
Right-Hand Pane
Workspace Items
Plates
Plate Properties
Choosing the Plate Type
Defining Favorite Plate Types
Changing Plate Function
Tips
Tip Properties
Tip Availability
Reagents
Reagent Properties
Configuring Reagents
Required Reagent Volume
Samples and Reactions
Sample Properties
Reaction Properties
Common Properties and Operations
Entering Sample Bar Codes
Data Transfer Formats
Importing Sample and Reaction Data
Exporting Sample and Reaction Data
User Management
User Identification
User Classes
Enabling Access Control
Managing User Groups
Change Current User
Revert to First User
Calibration
Calibrating Plate Positions
Calibrating Plate Heights
Calibrating Volumes
Calibration Management
Saving Calibration Data
Reinstating Calibration Data
Deleting Calibration Data
Run Files
Working with Run Files
Template Protocol Run Files
Obtaining Run Files
Opening New or Existing Run Files
Saving Run Files
Creating a Run File with the "Vacuum Extraction" Wizard
Starting the "Vacuum Extraction" Wizard
Vacuum Extractor Configuration Wizard" Step
Configuration [1]" Step
Configuration [2]" Step
Load Pre-Capture Reagent 1" Step
Load Capture Plate" Step
Wash Step 1 to Wash Step N" Step
Dry Sample" Step
Elution Steps" Step
Wizard Summary
Specifying Vacuum Processing
Configuring Tip Air Intake
Setting Run Parameters
Configuring Pipet Speed
Using Liquid-Level Tracking
Configuring Error Reporting
Setting Tip Use Options
Setting the Default File Location
Resetting to Default Values
Saving Current Settings as New Defaults
Setting System Color Configuration
Controlling Communication and Accessories
Generating Filenames for Reaction Data Export
Selecting a COM Port
Setting Shutdown Beep
Setting the Screen Saver Operation
Using the Service Register
Using the UV Lamp
Using the HEPA Filter
6 Operating Procedures
Switching on the Qiacube HT
Assembling the Vacuum Chamber
For Plates from Qiacube HT Kits
For Capture Plate VX or Capture Plate DX
Qiacube HT Protocols
Performing a Protocol
Pre-Run Report
Pre-Run Check Errors and Warnings
In-Run Open System/Open Circuit Test
In-Run Plate Detection Test
In-Run Interactive Vacuum Steps
Modifying a Protocol Reaction List
Canceling a Protocol
Post-Run Report
7 Maintenance Procedures
Servicing
Annual Preventive Maintenance
Regular Maintenance Procedures
Emptying the Waste Bottle of the Vacuum Station
Cleaning the Qiacube HT
Cleaning the Instrument after Completing a Run
Cleaning the Instrument after Shutting down
Cleaning Instrument Worktable Accessories
Weekly Maintenance Procedure
Pipetting Head Service Kit
Replacing HEPA Filters
Replacing Filters on the Vacuum Station
Shutting down the Qiacube HT
8 Troubleshooting Guide
Run Setup Errors
Important Error Messages
Software Errors
Instrument Errors
Error Errors
9 Technical Specifications
Environmental Conditions
Operating Conditions
Transportation Conditions
Storage Conditions
Mechanical Data and Hardware Features
Instrument Dimensions and Weight
Worktable Capacity
Liquid Handling
Hardware and Software Features
HEPA Filter Unit
Instrument Lid
Tip Pickup Mode
Detect Tip Absence
Appendix A Advanced Settings
Automatically Skip to Next if Detect Tip Absence
Compensate for Liquid Loss (Reagents)
Allow Volumes >Tip Capacity (Special Operations)
Reactions Are Mixed by Default
Allow Preserved Well Indices
Set Anti-Drip Inhale Tip Position
Set Anti-Drip Exhale Tip Position
Aspirate Reagent with Minimum Tip Insertion (else at Base)
Use Slow (Viscous) Pre-Wet Speeds for Reagent
Set Reagent Post Air Dispense Pause (Msec)
Use Slow Move Speeds for Reagent (Post Eject)
Aspirate Sample with Minimum Tip Insertion (else at Base)
Set Sample Post Aspirate Tip Raise Speed (%)
Set Sample Post Aspirate Pause (Msec)
Set Sample Post MIX/Eject Speed (Msec)
Use Special Pipetting Parameters for Lysate Transfer
Set Special Pipetting Parameters for Lysate Transfer
Compensate for Liquid Loss (Non-Reagent)
Use Slow Move Speeds for Sample
Break Seal Using 2-Step Carriage Lift
Sample and Reaction Functions for Non-Wizard Protocols
Choosing an Accessory
Choosing the Tip Type
Working with Sample Banks
Configuring Reactions
Description of the Generic XML Format
Appendix B
Declaration of Conformity
Waste Electrical and Electronic Equipment (WEEE)
FCC Declaration
Liability Clause
Appendix C - Ordering Information
Appendix D - Informations de Sécurité
Utilisation Appropriée
Sécurité Électrique
Sécurité Biologique
Échantillons
Produits Chimiques
Vapeurs Toxiques
Elimination des Déchets
Dangers Mécaniques
Sécurité Relative à la Maintenance
Symboles Sur le Qiacube HT
Appendix E - Sicherheitshinweise
Sachgemäße Handhabung
Schutz vor Stromschlag
Biologische Sicherheit
Proben
Chemikalien
Giftige Dämpfe
Entsorgen von Abfällen
Gefahren durch Mechanische Teile
Sicherheitshinweise - Wartungsarbeiten
Symbole auf dem Qiacube HT
Document Revision History
Advertisement
Quick Links
Download this manual
June 2023
QIAcube
HT
®
Version 4.17
User Manual
For QIAcube HT and QIAxtractor
®
Sample to Insight
Table of
Contents
Previous
Page
Next
Page
1
2
3
4
5
Advertisement
Table of Contents
Need help?
Do you have a question about the QIAcube HT and is the answer not in the manual?
Ask a question
Questions and answers
Related Manuals for Qiagen QIAcube HT
Laboratory Equipment Qiagen QIAcube ht User Manual
For qiacube ht and qiaxtractor (311 pages)
Medical Equipment Qiagen QIAcube HT User Manual
For use with the qiacube ht prep manager software (282 pages)
Laboratory Equipment Qiagen QIAgility User Manual
(334 pages)
Laboratory Equipment Qiagen QIAgility User Manual
(223 pages)
Laboratory Equipment Qiagen QIAcube User Manual
Sample & assay technologies (162 pages)
Laboratory Equipment Qiagen QIAcube Connect User Manual
(170 pages)
Laboratory Equipment Qiagen QIAcube Connect Safety Instructions And Quick Start Manual
For use with q-base (56 pages)
Laboratory Equipment Qiagen QIAcube Connect User Manual
(180 pages)
Laboratory Equipment Qiagen QIAamplifier 96 User Manual
(122 pages)
Laboratory Equipment Qiagen QIAreach eHub User Manual
(40 pages)
Laboratory Equipment Qiagen QIAxtractor User Manual
(207 pages)
Laboratory Equipment Qiagen PyroMark Q96 ID User Manual
(126 pages)
Laboratory Equipment Qiagen PyroMark Q24 MDx User Manual
(104 pages)
Laboratory Equipment Qiagen EZ1 Advanced User Manual
(126 pages)
Laboratory Equipment Qiagen BLU-V User Manual
For neutralization of dna of dead organisms (44 pages)
Laboratory Equipment Qiagen BioRobot M48 User Manual
(121 pages)
This manual is also suitable for:
Qiaxtractor
Table of Contents
Print
Rename the bookmark
Delete bookmark?
Delete from my manuals?
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL
Need help?
Do you have a question about the QIAcube HT and is the answer not in the manual?
Questions and answers