Qiagen PyroMark Q96 ID User Manual page 22

General Description
6. Once the temperatures and pressure reach the preset
7. Enzyme and substrate mixtures are predispensed into the
8. The reagent cartridge is positioned over each well in turn
9. The dispensation pressure is increased.
10. The nucleotides are predispensed into the rectangular
11. The reagent cartridge is positioned over each well in turn
12. The CCD camera collects data simultaneously from all
13. In wells where there is a positive reaction with the added
14. A period of 65 seconds (cycle time) is allowed between
15. The programmed sequence of nucleotide addition
3-4
levels (this may take several minutes), the
predispensation and dispensation of the reagents begins.
rectangular sink-well at the right-hand edge of the
PyroMark Q96 Plate Low to ensure that the dispensation
capillaries are flushed and filled with solution.
and the enzyme mixture is dispensed. The process is then
repeated for the substrate mixture.
Note: Dispensation of all reagents into the PyroMark
Q96 Plate Low is in a zigzag fashion. The mixing system
(vibrating process chamber) is operational throughout the
sequencing run.
sink-well at the right-hand edge of the PyroMark Q96
Plate Low.
and the programmed nucleotide is dispensed.
wells throughout the process. The CCD camera is cooled
to approximately 0°C to optimize performance and
reduce background noise.
nucleotide, light is emitted, giving rise to a peak in the
Pyrogram ® .
the addition of each nucleotide to ensure that all
enzymatic reactions are completed.
continues until the end of the program has been
reached.
PyroMark Q96 ID User Manual 01/2016