Glossary - Qiagen PyroMark Q96 ID User Manual

9

Glossary

Term Description
Biotin
A molecule that can bind very strongly to streptavidin. PCR
primers can be biotinylated to enable the resulting PCR
product to bind to streptavidin-coated Sepharose beads.
Dispensation Defines the nucleotides and the order in which they should
order be dispensed in Pyrosequencing runs.
Drop off
A continual decrease in signal height normally seen in the
Pyrogram.
Enzyme
A protein (or RNA) working as a catalyst, to enhance the
speed of a biochemical reaction without altering it. In
Pyrosequencing technology, a mixture of Klenow
polymerase, sulfurylase, luciferase, and apyrase is used in
the sequencing reaction.
Histogram
The theoretical representation of the expected
Pyrosequencing peak pattern.
Homopolymer
A stretch of identical bases in DNA. In Pyrosequencing
technology, a stretch of more than two identical bases is
regarded as a homopolymer.
Instrument A method that describes physical settings for the
parameters instrument, such as mixer frequency, block temperature,
and pulse time settings.
PyroMark Q96 ID User Manual 01/2016
Glossary
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