Qiagen PyroMark Q96 ID User Manual page 43

3. Switch on the vacuum pump.
4. Apply vacuum to the tool by opening the vacuum switch.
5. Wash the filter probes by lowering the probes into high-
6. Refill Parking Position with 180 ml high-purity water.
7. Close the vacuum switch on the worktable (Off) and
8. Add 40 µl of 0.4 µM sequencing primer in Annealing
PyroMark Q96 ID User Manual 01/2016
purity water (Parking Position). Flush the probes with 180
ml high-purity water.
Ensure that the water is being transferred to the waste
container. If it is not, ensure the tubing is connected
correctly and is not broken. Broken tubing should be
replaced, see Section 7.9.
Ensure that the tubing is connected properly and there is
no leakage.
place the tool in the Parking Position.
Buffer to each well of the PyroMark Q96 Plate Low that is
to be used.
Operating Procedures
5-11