Setting Up A Run - Qiagen PyroMark Q96 ID User Manual

Optional
If desired, enter a note about the assay and set up the
variable positions in the "Variable Positions" tab.
If creating a CpG assay, it is recommended that bisulfite
treatment controls are added. In the sequence before
bisulfite treatment, check if the suggested bisulfite controls
are Cs converted to Ts (read as Gs and As in a reverse
assay) and are suitable for controls or not.
To add a control, left-click a bold, orange T or A in the
histogram, preferable at the beginning of the sequence.
If applicable, during the assay validation, edit the analysis
parameters.
5.3.2

Setting up a run

1. In the shortcut browser, right-click the folder you want to
2. Click
3. In the "Instrument Parameters" drop-down list, select the
4. Optional: Enter the "Reagent ID", "Plate ID", plate "Bar
5. Add an assay to each well used, e.g., drag an assay
PyroMark Q96 ID User Manual 01/2016
place the run file in, and select "New Run" from the
context menu.
Note: To add a shortcut to a folder in the shortcut
browser, click "Add Folder Shortcut".
in the toolbar to enter a run name and save the
file in the desired folder.
instrument parameters file that corresponds to the
PyroMark Q96 Cartridge to be used.
code", or a "Run note".
from the shortcut browser to a well or a selection of
wells. A well is colored according to the assay loaded
into the well. Alternatively, right-click a well or selection
of wells and select "Load Assay".
Operating Procedures
5-3