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8
Troubleshooting
If you need to contact QIAGEN Technical Services about an
error, note down the steps leading to the error and any
information given in any dialog boxes. This will help the
QIAGEN Instrument Service Specialist in solving the problem.
When calling QIAGEN Technical Service about errors, please
have the following information ready:
Take the following action before contacting QIAGEN
Technical Services.
1. Check important parameters in the "Run Overview" in
2. Consult the Troubleshooting sections below.
8.1
Unexpected results
a) No result in any
wells of the plate
PyroMark Q96 ID User Manual 01/2016
Instrument serial number, type, and version
Date of last maintenance performed
Error code (if applicable)
Time point when the error occurred for the first time
Frequency of error occurrence (i.e., intermittent or
persistent error)
Photo of error, if possible
the "Instrument Status" tab to assess if the system was
working properly during the run.
Comments and suggestions
Check the reagent cartridge and ensure that it is
correctly filled with reagents and/or nucleotides.
Check that the needles are not damaged or
obstructed and that the reagent cartridge cover is
closed.
Insufficient sample or incorrectly conditioned
sample (e.g., too much dsDNA or salt present).
Follow sample preparation instructions carefully
(see Section 5.4).
Check the PCR samples using a gel technique to
confirm there is only one specific band.
Glossary
8-1
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