General Description; Pyromark Q96 Id Definitions; Pyrosequencing Principle - Qiagen PyroMark Q96 ID User Manual

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General Description

The PyroMark Q96 ID is a multi-application sequence
analysis system which uses Pyrosequencing
the real-time analysis of specific DNA sequences, including
single nucleotide polymorphisms (SNPs), mutation, and
methylation analysis. The instrument can analyze 96 samples
simultaneously. An easy-to-use protocol is used to prepare
samples.
Enclosed in the light-proof instrument housing, a light
detection system with cooled charged coupled device (CCD)
camera detects light emitted from reactions in the wells of the
PyroMark Q96 Plate Low. The instrument includes an
embedded computer module with hard disk and network
interface.
3.1

PyroMark Q96 ID definitions

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3.2

Pyrosequencing principle

Pyrosequencing uses sequencing by synthesis for accurate
and quantitative analysis of DNA sequences.
1. A sequencing primer is hybridized to a single-stranded,
2. The template is incubated with enzymes and substrates.
3. The first of four nucleotides is added to the reaction. If
PyroMark Q96 ID User Manual 01/2016
PyroMark Q96 ID Instrument: Instrument only
PyroMark Q96 Software: Software only
PyroMark Q96 Vacuum Workstation: Vacuum
Workstation only
PyroMark Q96 ID: Instrument, software, and installation
PyroMark Q96 ID System: All of the above, plus any
PyroMark kits
PCR-amplified DNA template.
the nucleotide is complementary to the base in the
template strand it will be incorporated into the DNA
strand by the DNA polymerase.
General Description
® technology for
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