Qiagen PyroMark Q24 MDx Manuals

Qiagen PyroMark Q24 MDx Manuals

Manuals and User Guides for Qiagen PyroMark Q24 MDx. We have 1 Qiagen PyroMark Q24 MDx manual available for free PDF download: User Manual

Qiagen PyroMark Q24 MDx User Manual

Qiagen PyroMark Q24 MDx User Manual (104 pages)

Brand: Qiagen | Category: Laboratory Equipment | Size: 4 MB

Table of Contents

Table of Contents
3
Safety Information
7
Proper Use
7
Electrical Safety
8
Biological Safety
9
Chemicals
10
Mechanical Hazards
10
Heat Hazard
11
Consumables
11
Symbols on the Pyromark Q24 MDX and Pyromark Q24 Products
12
Introduction
15
About this User Manual
15
General Information
16
Technical Assistance
16
Policy Statement
16
Version Management
16
Intended Use of Pyromark Q24 MDX
17
Requirements for Pyromark Q24 MDX Users
17
General Description
19
Pyromark Q24 MDX Definitions
19
Pyrosequencing Principle
19
Pyromark Q24 MDX Principle
21
Pyromark Q24 MDX Instrument
22
Process Chamber
23
Dispensing Unit
23
Sample Preparation with the Pyromark Q24 MDX Vacuum Workstation
24
Analysis Software
24
Installation Procedures
25
System Delivery and Installation
25
Requirements
25
Installation of the Analysis Software
27
Installing or Upgrading Pyromark Q24 MDX Software
27
Uninstalling Pyromark Q24 MDX Software
28
Operating Procedures
29
Instrument Administration
29
Setting Date and Time
29
Copying Unsaved Runs
29
Copying Recently Saved Runs
30
Copying Log Files
30
Extracting Damaged Runs
31
Viewing Acknowledgements, Version, and Contact Information
31
Upgrading the Instrument Software
31
Run an External Application
32
Setting up a Run
32
Starting Pyromark Q24 MDX Software
32
Setting up an Assay
32
Setting up a Run
33
Managing Instrument Methods
35
Sample Preparation
36
Pyromark Q24 MDX Vacuum Workstation Function Test
37
DNA Amplification
38
Immobilizing the PCR Product to Beads
38
Separation of DNA Strands and Release of Samples into the Pyromark Q24 Plate
39
Annealing of Sequencing Primer to Samples
44
Preparation of Pyromark Gold Q24 Reagents
44
Processing a Run on the Pyromark Q24 MDX Instrument
45
Instrument Software
46
Starting the Instrument
46
Starting the Run
47
Monitoring the Run
48
After the Run
49
Analyzing the Run
52
Viewing the Analysis Results
53
Analysis Reports
55
Finishing Work and Shutting down
56
Shutting down the Instrument
56
Emptying the Waste Container and Troughs
57
Checking the Instrument
58
Backup of Pyromark Q24 Files
58
Maintenance
59
Checking the Performance of the Pyromark Q24 MDX
59
Maintenance of the Pyromark Q24 MDX Instrument
60
Cleaning the Instrument
60
Cleaning the Heating Block and Light Guides
61
Maintenance of the Pyromark Q24 MDX Vacuum Workstation
63
Cleaning the Pyromark Q24 MDX Vacuum Workstation
63
Testing and Replacing the Filter Probes
64
Replacing the Rubber Seal
65
Replacing the Tubing
66
Replacing the Waste Filter
67
Troubleshooting
69
Analysis-Related Errors
70
Analysis Software-Related Errors
72
Instrument-Related Errors
73
Vacuum Workstation-Related Errors
74
Verification of Correct Installation and Operation
75
Glossary
77
Technical Data
83
Environmental Conditions
83
Waste Electrical and Electronic Equipment (WEEE
87
FCC Declaration
88
EC Declaration of Conformity
89
Assay Design and Validation
90
Liability Clause
99

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