Sample Preparation - Qiagen PyroMark Q96 ID User Manual

Operating Procedures
Instrument parameters
In the "Instrument Parameters" dialog box, the following
parameters are available.
Reagent pressure
Enzyme pulse time
Substrate pulse time
Nucleotide pressure
Nucleotide pulse
time
Note
5.4

Sample preparation

Equipment and reagents to be supplied by the user
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5-6
PyroMark Q96 Vacuum Workstation
PyroMark Q96 Plate Low (cat. no. 979002)
PyroMark 96 Sample Prep Thermoplate Low (supplied
with the PyroMark Q96 ID)
Mixer for immobilization to beads
Heating block capable of attaining 80°C
96-well PCR plate and adhesive foil or strip caps
Biotinylated PCR template
Streptavidin Sepharose
17-5113-01)
Sequencing primer
High-purity water (Milli-Q 18.2 MΩ x cm or equivalent)
Ethanol (70%)
PyroMark Q96 ID User Manual 01/2016
Pressure (millibar) for
dispensation of the enzyme
mix and substrate mix.
Dispensation time
(milliseconds) for the enzyme
mix.
Dispensation time
(milliseconds) for the
substrate mix.
Pressure (millibar) from the
dispensation of nucleotides.
Dispensation time
(milliseconds) for nucleotides.
Note about the instrument
method (optional).
® HP (GE Healthcare, cat. no.