Qiagen PyroMark Q96 ID Manuals

Manuals and User Guides for Qiagen PyroMark Q96 ID. We have 1 Qiagen PyroMark Q96 ID manual available for free PDF download: User Manual

Qiagen PyroMark Q96 ID User Manual (126 pages)

Brand: Qiagen | Category: Laboratory Equipment | Size: 1 MB

Table of Contents
3
Safety Information
9
Proper Use
9
Electrical Safety
10
Chemical Hazards
11
Mechanical Hazards
11
Heat Hazards
12
Symbols Used on the Pyromark Q96 ID
12
Introduction
15
About this User Manual
15
General Information
16
Technical Assistance
16
Policy Statement
16
Version Management
16
Intended Use of the Pyromark Q96 ID
17
Requirements for Pyromark Q96 ID Users
18
General Description
19
Pyromark Q96 ID Definitions
19
Pyrosequencing Principle
19
Pyromark Q96 ID Principle
21
External Features of the Pyromark Q96 ID
23
Internal Features of the Pyromark Q96 ID
24
Process Chamber
24
Dispensing Unit
24
Controls and Indicators
25
Front Panel
25
Rear Panel
25
Computer and Software
26
Installation Procedures
27
Instrument Delivery and Installation
27
Requirements
27
Moving the Pyromark Q96 ID
29
Reinstalling the Pyromark Q96 ID: Stand Alone
30
Reinstalling the Pyromark Q96 ID: Network Installation
30
Connecting Mains Power
31
Setting up Network Properties
31
Used IP Addresses for Instrument and Computer
31
Operating Procedures
33
Required Items
33
Starting the System
33
Starting the Pyromark Q96 ID
33
Starting the Pyromark Q96 Software
34
Preparing a Run
34
Setting up an Assay
34
Setting up a Run
35
Managing Instrument Parameters
36
Sample Preparation
38
Assembly of the Pyromark Q96 Vacuum Workstation
39
DNA Amplification
39
Immobilization of the PCR Product to Beads
40
Strand Separation
41
Annealing of Sequencing Primer to Samples
46
Reagent Preparation
46
Run
47
Loading the Pyromark Q96 Plate Low
47
Loading the Reagent Cartridge
48
Starting the Run
49
Monitoring the Run
49
Post-Run Procedures
50
Analyzing the Run
50
Viewing the Analysis Results
51
Analysis Reports
54
Finishing Work
55
After a Run Is Finished
55
Leaving the Instrument on
55
Exiting Pyromark Q96 Software
56
Cleaning the Instrument
56
Emptying the Waste Container and Troughs
56
Pyromark Q96 Software
59
System Requirements
59
Operator's and Office Computers
59
Printer
59
Data Storage Device
59
Installing Pyromark Q96 Software
59
Uninstalling Pyromark Q96 Software
60
Upgrading from Pyromark Q96 ID Software
60
Installing the Pyromark Data Converter Tool
61
Using the Pyromark Data Converter Tool
61
Data Backup
63
Installation Testing
63
Maintenance
67
Shutdown Procedure
68
Routine Maintenance of the Pyromark Q96 ID
68
Cleaning the Lens Array
69
Cleaning the Heating Block
69
Changing the Fuses
70
Cleaning and Testing the Reagent Cartridge
71
Standard Cleaning Procedure
71
Clogged Cartridge Cleaning Procedure
72
Cartridge Function Check
72
Cleaning the Pyromark Q96 Vacuum Workstation
74
Testing and Replacing the Filter Probes
75
Function Test for Filter Probes
75
Replacing Filter Probes
76
Replacing the Tubing
76
Glossary
85
Technical Data
89
Environmental Conditions
89
Waste Electrical and Electronic Equipment (WEEE
92
FCC Declaration
93
Assay Design and Validation
97

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Qiagen PyroMark Q96 ID Manuals

Qiagen PyroMark Q96 ID User Manual (126 pages)

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