Qiagen PyroMark Q96 ID User Manual page 44

Operating Procedures
9. After shaking, place the PCR plate and PyroMark Q96
10. Apply vacuum to the tool by opening the vacuum switch.
11. Carefully lower the filter probes into the PCR plate to
12. Ensure that all liquid is aspirated from the wells and that
13. Transfer the tool to the trough containing 70% ethanol
14. Transfer the tool to the trough containing Denaturation
15. Transfer the tool to the trough containing Wash Buffer
16. Raise the tool to beyond 90° vertical for 5 s, to drain
5-12
Plate Low on the worktable. Ensure that both plates are
in the same orientation as when samples were loaded.
Note: Sepharose beads sediment quickly. If more than
1 min has elapsed since the plate (or strips) was
agitated, agitate again for 1 min before capturing the
beads.
capture the beads containing immobilized template.
Hold the probes in place for 15 s. Take care when
picking up the tool.
all beads have been captured onto the filter probe tips.
Note: If the wells still contain liquid or white beads
remain, the filter probes may need replacing, see Section
7.8.2.
(trough 1). Flush the filter probes for 5 s.
Solution (trough 2). Flush the filter probes for 5 s.
(trough 3). Flush the filter probes for 10 s.
liquid from the filter probes (see image).
PyroMark Q96 ID User Manual 01/2016