Qiagen PyroMark Q96 ID User Manual page 45

17. While holding the tool over the PyroMark Q96 Plate Low,
18. Release the beads in the plate containing 0.4 µM
19. Transfer the tool to the trough containing high-purity
20. Wash the filter probes by lowering the probes into high-
21. Raise the tool to beyond 90° vertical for 5 s, to drain
22. Close the vacuum switch on the worktable (Off) and
23. If more than one plate is prepared at once, refill the
24. Turn off the vacuum pump.
Note: The filter probes should be left to air dry when not in
use, i.e., empty the trough and ensure there is no water in
the Parking Position when the tool is left there.
PyroMark Q96 ID User Manual 01/2016
close the vacuum switch on the worktable (Off).
sequencing primer in 40 µl Annealing Buffer by shaking
the tool gently from side to side in the plate. Allow the
filter probes to rest on the bottom of the wells.
water (trough 4) and agitate the tool for 10 s.
purity water (Parking Position) and applying vacuum.
Flush the probes with 180 ml high-purity water.
liquid from the filter probes.
place the tool in the Parking Position.
troughs (step 2) and repeat the procedure from step 8.
Operating Procedures
5-13