Qiagen PyroMark Q96 ID User Manual page 53

SQA assays
Quality assessments
The plate overview in the "Overview" tab summarizes quality
assessments. Select the "Quality" option in the upper right
corner to display the quality assessment of all variable
positions in a selected well or of all bases in the base-called
sequence. SQA assays also include quality assessment at the
end of the quality control window. Quality levels are
indicated with different colors:




AQ, SNP, and CpG analysis results
Allele frequencies (AQ analysis), genotypes (SNP analysis),
and methylation percentages (CpG analysis) are displayed at
PyroMark Q96 ID User Manual 01/2016
Select to show the methylation bar. The
methylation bar shows the methylation
level for each CpG site in the well.
Select to show the quality assessment at
the end of the quality control window.
The default number of bases included is
20.
Blue: Passed
Yellow: Check
Red: Failed
White: Not analyzed. Either analysis is not supported by
the software (e.g., analyzing single nucleotide
polymorphisms in CpG mode) or the variable position
has been deselected by the user (AQ, SNP, and CpG
assays only).
Operating Procedures
5-21