Pyromark Q96 Id Principle - Qiagen PyroMark Q96 ID User Manual

9. As the process continues, the complementary sequence is
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PyroMark Q96 ID principle

The PyroMark Q96 ID performs DNA sequencing using
Pyrosequencing technology.
1. After setting up the run in the PyroMark Q96 Software,
2. The run is then started when the user clicks the "Run"
3. The temperature of the heating block and chamber rises
4. The mixer speed adjusts to the preset level.
5. The pressure in the pneumatic dispensing system rises to
PyroMark Q96 ID User Manual 01/2016
built up and the nucleotide sequence is determined from
the peak in the Pyrogram.
the PyroMark Q96 Plate Low containing DNA samples is
placed on the heating block in the instrument and the
reagent cartridge containing enzymes, substrate, and
nucleotides is placed in the dispensing unit.
button in the "Run Setup" dialog box. The operator's
computer sends all information required for the analysis
to the instrument.
Note: During a run, data are continuously stored on the
operator's computer and on the computer embedded in
the instrument. If the communication between the
instrument and the operator's computer is interrupted
during the run, data from the run will be automatically
restored once communication has been re-established.
to preset levels.
the preset level.
General Description
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