Qiagen QIAgility User Manual
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Qiagen QIAgility User Manual

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June 2023
QIAgility
Version 4.18
®
User Manual
Sample to Insight

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Summary of Contents for Qiagen QIAgility

  • Page 1 June 2023 QIAgility Version 4.18 ® User Manual Sample to Insight...

  • Page 2: Table Of Contents

    General information ........................... 10 Technical assistance ........................10 Policy statement ......................... 10 Version management ......................... 10 Intended use of the QIAgility ....................... 11 Requirements for QIAgility users ....................11 Safety Information ............................12 Proper use ............................12 Electrical safety ..........................13 Biological safety ..........................
  • Page 3 Installing the QIAgility Software ....................34 Uninstalling QIAgility software ....................39 Folders ............................. 40 “QProtocols” folder ........................41 Connecting the computer to the QIAgility ..................41 COM port configuration ......................42 Installation of AC power cord ..................... 42 Hardware Wizard ..........................43 Setting up the QIAgility ........................
  • Page 4 Plate operations ........................76 Setting tip availability ......................... 81 Managing samples ..........................82 Managing sample banks ......................82 View and edit information using the right-hand pane ................86 Tip ............................86 Standards ..........................86 Reagents ..........................89 QIAgility Version 4.18 User Manual 06/2023...
  • Page 5 Pre-run report .......................... 156 Post-run report ......................... 157 Log files and support packages ....................158 Importing and exporting reaction/sample data ................... 160 Shutting down the software and the instrument ..................161 Instrument Setup and Calibration ........................ 163 QIAgility Version 4.18 User Manual 06/2023...
  • Page 6 Calibrate tip offsets ........................170 Calibrate volumes ........................172 Maintenance Procedures ..........................176 Regular maintenance procedures ....................... 177 Cleaning the QIAgility ......................177 Periodic maintenance ........................178 Decontaminating the QIAgility ....................178 Removing and replacing the tip ejector chute ................179 Replacing the HEPA filter ......................
  • Page 7 Use optimal height calibration where possible ..................196 Optimal plate height calibration ......................196 Description of the Generic XML Format ......................200 Packing the QIAgility ..........................202 Appendix B ................................ 203 Declaration of Conformity .......................... 203 Waste Electrical and Electronic Equipment (WEEE) ..................203 FCC declaration ............................
  • Page 8 Sécurité relative à la maintenance ....................... 212 Symboles sur le QIAgility ........................... 213 Appendix E – Sicherheitshinweise ......................... 214 Sachgemäße Handhabung ......................... 214 Elektrische Sicherheit ..........................216 Biologische Sicherheit ..........................216 Proben 217 Chemikalien ............................. 217 Giftige Dämpfe ............................ 217 Abfallentsorgung ............................

  • Page 9: Introduction

    Thank you for choosing the QIAgility. We are confident it will become an integral part of your laboratory. Before using the QIAgility, it is essential that you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition.

  • Page 10: General Information

    QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques.

  • Page 11: Intended Use Of The Qiagility

    If used in combination with QIAGEN kits indicated for use with the QIAgility, the instrument is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAgility is used with other than QIAGEN kits, it is the user’s responsibility to validate the performance of the product combination for any particular application.

  • Page 12: Safety Information

    Safety Information Before using the QIAgility, it is essential that you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition.

  • Page 13: Electrical Safety

    The robotic arm moves during position calibration while the instrument lid is raised. Never click any buttons while parts of your body are within the instrument workspace. In case of emergency, switch off the QIAgility at the power switch at the rear of the instrument and unplug the power cord from the power outlet.

  • Page 14: Biological Safety

    If liquid has spilled inside the instrument, switch off the instrument, disconnect it from the power outlet, and contact  QIAGEN Technical Services. If the instrument becomes electrically unsafe, prevent other personnel from operating it, and contact QIAGEN Technical  Services. The instrument may be electrically unsafe when: The line power cord appears to be damaged.

  • Page 15: Chemicals

    For information on use and disposal of the tip disposal box, please see Section 3.2.6, page 21. For information about how to dispose of the QIAgility at the end of its economic life, see Section 10. Mechanical hazards...

  • Page 16: Maintenance Safety

    Wait until the robotic arm has completed the current movement. This may take up to 10 seconds. Maintenance safety Perform the maintenance as described in Section 8. QIAGEN charges for repairs that are required due to incorrect maintenance. WARNING/...

  • Page 17: Symbols On The Qiagility

    Type plate on the back of the RoHS mark for China (the restriction of the use of certain instrument hazardous substances in electrical and electronic equipment) Type plate on the back of the Consult instructions for use instrument QIAgility Version 4.18 User Manual 06/2023...

  • Page 18: General Description

    PCR setup. The instrument configuration is with HEPA filter/and UV lamp. QIAgility principle The QIAgility uses a single-channel pipet to automate PCR setup — up to 96 PCR reactions can be set up in approximately 30 minutes. Level sensing, facilitated by conductive tips, enables high-precision pipetting.

  • Page 19: The Qiagility

    UV lamp Instrument lid (1) CAUTION Damage to the QIAgility lid Do not use alcohol, solvents, reagents containing acids, alkalis, or abrasives to clean the instrument lid. For more information, see Section 8.1.1. The lid of the instrument provides both an isolated enclosure for performing purification and a safety mechanism.

  • Page 20: Feet (2)

    Tip chute. Tips used on the worktable are discarded through the tip chute. Located at the left hand side of the QIAgility, the tip chute is held in place by a locating block, allowing easy placement and removal for cleaning.

  • Page 21: Hepa Filter (4)

    The UV lamp does not irradiate certain areas, including the pipetting head, due to construction constraints. The UV lamp is enabled and time of exposure is set within the QIAgility Software. For more details, see Section 6.2. Pipetting head (6) and robotic arm Liquid-handling tasks are performed by the pipetting head, which provides a motor-driven, backlash-compensated pipetting mechanism.

  • Page 22: Worktable

    The worktable is the instrument surface upon which items, such as plates, reagents, and tips are placed. Worktable layout is specified for a protocol with a run file and is represented in the QIAgility Software as the workspace. For more details, see Section 5.3.4.

  • Page 23: Internal Features Of The Qiagility

    Internal features of the QIAgility Worktable Worktable Master Mix Block Master Mix Block Reagent Block Reagent Block Tip Rack Holders and tips Tip Rack Holders and tips Y-arm Y-arm Tip ejector Tip ejector X-axis The X-axis directs the left/right movement of the pipetting head.
  • Page 24 Master Mix Block and Reagent Block. The silver paint is epoxy paint which is hard wearing and UV resistant. Master Mix Block The master mix block is intended to hold diluent and master mix on the worktable. The QIAgility is supplied with 2 Master Mix Blocks which can hold: 5 x 5 mL free-standing tubes ...
  • Page 25 The Reagent Block is intended to hold reagents and standards on the worktable. The Reagent Block can hold 8 x 1.5 mL tapered tubes, 8 x 2 mL free-standing tubes, and 16 x 200 µL PCR tubes. Adapters The QIAgility is supplied with 3 SBS adapters: 2 x Adapter, 96 x 0.2 mL PCR Tubes ...
  • Page 26 Sample tubes A range of tube types can be used with the QIAgility. The following sample tubes are supplied with the instrument: 5 mL free-standing tubes  200 µL PCR tubes  Note: Supplied sample tubes are not guaranteed to be sterile.

  • Page 27: Liquid-Level Tracking

    The QIAgility is operated via a computer. A laptop with the correct specification for operation of the QIAgility and QIAgility Software is supplied as part of the QIAgility System. However, if a different computer is used to operate the QIAgility or to run the QIAgility Software, then the following laptop hosting systems are recommended.
  • Page 28 The computer connects to the QIAgility by the USB. Note: The software is tested by QIAGEN using the original image supplied with your laptop without connecting the laptop to the internet. Updates on your operating system might lead to unexpected behavior of the QIAgility software.

  • Page 29: Installation Procedures

    The QIAgility should be installed in a laboratory environment that is free of excessive airborne dust.  Note: We recommend that the QIAgility does not share a wall socket with other laboratory equipment, and that the QIAgility is plugged directly into its own wall socket.

  • Page 30: Laptop

    Important: The wall socket should be easily accessible. Important: Position the QIAgility in a way that allows easy access to the power supply cable at the rear. Meeting these location and position requirements will allow you to immediately disconnect the instrument from the power supply should a need or emergency arise.

  • Page 31: Unpacking The Qiagility

    Services. Make sure that the QIAgility has equilibrated to ambient temperature before operating it. Retain the packaging in case you need to transport or ship the QIAgility in the future. Instructions for packing the QIAgility are outlined in Appendix A – Packing the QIAgility. Using the original package minimizes damage during transportation of the QIAgility.

  • Page 32: Installing The Qiagility

    Note: The procedure for removing the transport locks is also written on the transport locks on the instrument. 1. Unscrew the two screws (1 and 2) and remove the horizontal bar. 2. Move the pipetting head to the right and remove the transport lock from the worktable. QIAgility Version 4.18 User Manual 06/2023...

  • Page 33: Uv Lamp

    Note: Instructions for exchanging the HEPA filter system are given in Section 8.2.3. Instrument calibration Before the instrument is used, some calibrations are required: position and height calibrations, tip offset calibration, and volume calibration. Please refer to Sections 7.1.2 to 7.1.6 for detailed descriptions. QIAgility Version 4.18 User Manual 06/2023...

  • Page 34: Computer Setup And Installation Of Software

    For computer system requirements, please see Section 3.4. 1. Set up the computer as described in the computer installation instructions provided with the computer. 2. Before installing the QIAgility Software, shut down all other software and make sure that the instrument is not connected to the computer.
  • Page 35 6. The “QIAgility Setup Wizard” will appear. This wizard will guide you through the installation. Instructions are displayed on each screen of the wizard. Note: We recommend using the default settings for all screens. 7. Click on the Next button to continue with the installation process. The “License Agreement” screen will appear.
  • Page 36 10. To uninstall a previous software version, click on the Yes button. The calibration settings and run files will be retained. To maintain the previous version of the software, click on the No button and browse for a different installation directory. Click on the Next button to continue. QIAgility Version 4.18 User Manual 06/2023...
  • Page 37 12. Select the additional tasks and then click on the Next button. Select Create a desktop icon to create a desktop icon to easily launch the software. Select Create a Quick Launch icon to generate a quick launch icon. 13. Review the displayed details. Click on the Install button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 38 14. The QIAgility Software will now be installed onto your computer. This could take a few minutes. 15. If you are using Windows 10, the following message will appear. For correct installation of USB drivers, click on the Finish button.

  • Page 39: Uninstalling Qiagility Software

    16. Review the displayed information, and then click on the Next button to continue with the software installation. 17. To directly launch the QIAgility Software, select Launch QIAgility and then click on the Finish button. If you do not want to launch the QIAgility software immediately, click on the Finish button to complete the installation process.

  • Page 40: Folders

    Q Protocols are stored in this folder; see Sections 4.7.1 and 6.4.1 for more details about this folder. In addition, these folders are important for operating the instrument: AccDefs  Libs  Patches  PlateDefs  PlateImg  PresetMasterMix  Security  Settings  SocketDefs  TableDefs  TubeDefs  QIAgility Version 4.18 User Manual 06/2023...

  • Page 41: Qprotocols" Folder

    QProtocols folder, it will not be listed when the Protocols tab is selected. Connecting the computer to the QIAgility Note: Before connecting the QIAgility to the computer for the first time, ensure that the QIAgility Software has been installed (see Section 4.5.4).

  • Page 42: Com Port Configuration

    1. Ensure that the power switch of the QIAgility is set to the off position. 2. Check that the voltage rating on the label at the back of the QIAgility matches the voltage available at the installation site. 3. Plug the power cord into the main power input socket.

  • Page 43: Hardware Wizard

    Hardware Wizard 1. Select No, not this time, and click Next. 2. We recommend that you select Install the software automatically. 3. Click on the Next button to continue. QIAgility Version 4.18 User Manual 06/2023...

  • Page 44: Setting Up The Qiagility

    1. Ensure that the instrument lid is closed. 2. Set the power switch of the QIAgility to the on position and switch the computer on. The pipetting head should move upwards. If this does not happen, please consult the Troubleshooting section of this user manual.
  • Page 45 7. Click on the OK button to proceed to tip ejector calibration. See Section 7.1.1 for more details about how to do this. Note: Tip ejector calibration must be performed before any other calibrations. 8. Perform the remaining calibrations in the order listed in Sections 7.1.2 to 7.1.6. QIAgility Version 4.18 User Manual 06/2023...

  • Page 46: Qiagility Software

    Starting the software Note: Before starting the QIAgility Software, ensure that the instrument lid is closed and that the instrument is switched on. 1. Double-click on the icon on the desktop, or select Start > Programs > QIAgility > QIAgility. If the instrument is switched on, and is connected to the computer, the software will proceed automatically to “Normal Mode”...

  • Page 47: Virtual Mode

    The main software window provides an interface for the creation of run files. The main features are as follows: Menu bar  Toolbar  Mode indicator  Software workspace  Menu bar Mode indicator Toolbar Software workspace QIAgility Version 4.18 User Manual 06/2023...

  • Page 48: Menu Bar

    Opens a description of the Q Protocol template which is currently loaded. This option is only available when a Q Protocol or User-Developed Protocol is loaded. Unlock protocol Unlocks the protocol which is currently loaded. This option is only available when a Q Protocol or User- Developed Protocol is loaded. QIAgility Version 4.18 User Manual 06/2023...
  • Page 49 Launches the “Optimisation Wizard”. This wizard enables specialized PCR functions to be set up. For example this wizard can be used for optimization of a PCR run (e.g., primer concentration). See Section 5.10.2 for further details. QIAgility Version 4.18 User Manual 06/2023...
  • Page 50 See Section 5.5. Calibration management Enables calibration settings to be saved using a range of storage devices (e.g., USB stick). See — Save calibrations on Section 5.5.1. setup disk QIAgility Version 4.18 User Manual 06/2023...
  • Page 51 Calibrates the pipetting head to the tip ejector. Enables correct tip ejection and ensures that the Setup tip ejector vacuum hub is not damaged. See Section 7.1.1. Robot setup — Enables selection of the COM port which is used for communication with the QIAgility. See Select COM port Section 5.11. Robot setup —...
  • Page 52 Enables the user to change the default file location for all saved files. See Section 5.6.5. Set default file location Run settings — Saves current software run settings as the new default settings. This does not include calibration Save current settings as settings. default QIAgility Version 4.18 User Manual 06/2023...
  • Page 53 Advanced settings Provides additional options for use in specialist applications. Only change these settings under direct instructions from QIAGEN. See Section 10 for more detailed information about these settings. Change plate type Launches the “Plate Configuration” dialog box, which enables the user to change the plate type in a selected position on the software worktable.

  • Page 54: Toolbar

    See Section 6.7.3. About QIAgility Displays information about the QIAgility, including the version number, serial number and firmware number. QIAGEN contact details, which may be required to place orders or to request technical support, are also displayed. Toolbar This section outlines functions that are available from the toolbar menu.
  • Page 55 Opens a protocol description for a Q Protocol. Indicates that the run file which is currently open is a locked Q Protocol. Indicates that the run file which is currently open is a locked User-Developed Protocol. QIAgility Version 4.18 User Manual 06/2023...

  • Page 56: Mode Indicator

    C1, and C2. Positions for the Master Mix Block and Reagent Block are labeled M1 and R1. Status bar The status bar displays the current status of the instrument during a run. Software version This bar displays the current software version. QIAgility Version 4.18 User Manual 06/2023...
  • Page 57 Plate buttons are located in the top right corner of the schematic plates on the main software window. They provide information about the plates and appear when the cursor is moved over a plate. QIAgility Version 4.18 User Manual 06/2023...
  • Page 58 Click on the right-hand pane and then drag to increase or decrease the size of the right-hand pane in a vertical or horizontal direction. Minimize Minimizes the right-hand pane. Maximize Maximizes the right-hand pane. QIAgility Version 4.18 User Manual 06/2023...
  • Page 59 Please refer to Section 5.9.5 for detailed information about the “Sample” right-hand pane. Reactions Please refer to Section 5.9.6 for detailed information about the “Reaction” right-hand pane. Progress report Please refer to Section 5.9.8 for more detailed information about the “Progress report” right-hand pane. QIAgility Version 4.18 User Manual 06/2023...

  • Page 60: User Management

    User management User management allows the definition of different access rights to various data and functions within the QIAgility Software. User management functions are accessed from the menu bar (Options > User Management). User identification Unlike software that requires separate login authentication, the QIAgility Software user is defined as the current Windows user logged on the laptop on which the software is installed.
  • Page 61 Upon restarting the software, only Administrators and users assigned to the “Manager”, “Designer” and “Operator” groups can operate the software. When attempting to start the QIAgility Software, users that are not assigned to a user groups are denied access to the software.

  • Page 62: Enabling Access Control

    Enabling access control Upon installation, the QIAgility Software user access control features are disabled and all users have “Manager” access. (See the previous section for user group descriptions.) A Manager enables the user access control with Options > User management > Enable access control.

  • Page 63: Change Current User

    Alternatively, select Options > User management > Change Current User. The “Change User” window appears. The new user must enter their username and password, and click OK to log on. If the login was successful the new rights will take effect immediately. QIAgility Version 4.18 User Manual 06/2023...

  • Page 64: Revert To First User

    3. A default filename and location will be supplied. If desired, these can be changed. 4. Click on the Save button. The current calibration settings are now saved on the “Setup Disk” and can be transferred to another computer. QIAgility Version 4.18 User Manual 06/2023...

  • Page 65: Load Calibrations From Setup Disk

    1. Select Options > Calibration management > Delete multiple calibrations. 2. The “Delete Multiple Calibrations” dialog box will appear. 3. Select the calibration settings to be deleted. 4. Click on the Delete button. The selected calibrations will now be deleted. QIAgility Version 4.18 User Manual 06/2023...

  • Page 66: Configure Run Settings

    Note: Larger air volumes may be beneficial when pipetting viscous liquids. Smaller air volumes may be beneficial when pipetting smaller volumes, or when dispensing into small tubes. 4. Click on the OK button to return to the main software window. QIAgility Version 4.18 User Manual 06/2023...

  • Page 67: Configure Pipet Speed

    Adjusting the minimum extra volume and per-reaction volume from their default settings can be useful when preparing master mix for manual PCR setup, or when preparing master mix for PCR setup at a later time. QIAgility Version 4.18 User Manual 06/2023...

  • Page 68: Set Standard Extra Volume

    3. Enter a new extra volume. Minimum extra Defines the additional volume which is prepared for each concentration of the standard volume required dilution. 4. Click on the OK button to return to the main software window. QIAgility Version 4.18 User Manual 06/2023...

  • Page 69: Set Concentration Unit

    If deactivated, the user sees the simple reagents table in the reaction configuration form. If multiple volumes for at least one reagent are used, and the user tries to deactivate the option, a message box appears: QIAgility Version 4.18 User Manual 06/2023...

  • Page 70: Set Default File Location

    Set default file location The default file location for all saved files is C:\Program Files\QIAgility\Data. Run files are stored in this directory. This directory contains three subdirectories — Logs, Reports, and SupportPackages. See Section 4.7 for more details about these subfolders.
  • Page 71 Sample Colours Liquid Colours QIAgility Version 4.18 User Manual 06/2023...
  • Page 72 Resets all sample colors. Click on the “Yes” button to continue. Refreshes the colors displayed on the software worktable according to the current settings. Opens color theme. Saves color theme. Closes and applies updated colors. QIAgility Version 4.18 User Manual 06/2023...

  • Page 73: Adjust Liquid Level-Tracking Options

    (i.e., “Do not ignore errors”), an error message will appear every time an aliquot is taken from the tube. To avoid the need for user intervention every time an aliquot is taken, a different error level can be selected. QIAgility Version 4.18 User Manual 06/2023...

  • Page 74: Set Tip Usage Options

    Use each tip for one One tip will be used for each aspirate/eject cycle. This option gives the highest accuracy operation only and precision, but requires a large number of tips. Run duration will be increased. QIAgility Version 4.18 User Manual 06/2023...

  • Page 75: Configure Multi-Eject Options

    2. The “Tip Usage” dialog box will appear. See Section 5.6.11 for more details about configuring tip usage options. 3. Select “Re-use tips where possible”, or “Use each tip for one operation only”, and then click on the “Multi-Eject Settings” button. QIAgility Version 4.18 User Manual 06/2023...

  • Page 76: Define Worktable Setup

    Note: Setting this value to zero may reduce precision. It is recommended to set this volume to half the volume of the aliquot. Define worktable setup Plate operations The plate operations menu provides options to change plate type, change plate function, manage tip availability, and manage sample banks. QIAgility Version 4.18 User Manual 06/2023...
  • Page 77 See “Adding/removing an accessory” in this section. Change plate function Enables the plate function to be changed. A tick will appear next to the plate function that has been selected. See “Changing the plate function” in this section. QIAgility Version 4.18 User Manual 06/2023...
  • Page 78 This function is useful, for example, when it is convenient to dispense liquids by column (vertical pipetting order), but to export the data by row (horizontal label order). QIAgility Version 4.18 User Manual 06/2023...
  • Page 79 4. Select a plate type from the “Select Plate Type” drop-down menu. The “Plate” list will be automatically updated. 5. Select a plate from the “Plate” list. Note: If you cannot find a plate type that matches, or is very similar to the plate that you are using, please contact QIAGEN Technical Services.
  • Page 80 To add or remove an accessory: 1. Right-click on the plate where the accessory is to be added/removed. The plate operations menu will appear. 2. Select Change accessory. The “Select Accessory” dialog box will appear. QIAgility Version 4.18 User Manual 06/2023...

  • Page 81: Setting Tip Availability

    Note: To select individual tips, left-click on an individual tip and proceed as for a group, or use the tip availability short cut. Tip availability short cut Hold down “Alt” and left-click on individual tips to switch the tip between available and unavailable. QIAgility Version 4.18 User Manual 06/2023...

  • Page 82: Managing Samples

    Right-click on a “Sample” or “Reaction” plate to view sample bank options in the upper panel of the plate operations menu. Select any of these options, apart from Toggle sample bank list and Set preload volume, to directly display the “Update Sample Bank” dialog box. QIAgility Version 4.18 User Manual 06/2023...
  • Page 83 Enables the user to enter the volume of liquids which have been preloaded into reaction tubes. This option is only available when a “Reaction” plate is selected. Click on the OK button to continue. QIAgility Version 4.18 User Manual 06/2023...
  • Page 84 Click to view the existing wells in the sample bank. Wells are highlighted red. Specify concentration Select to define a concentration range for the filter. Specify the “Min Concentration”, “Max range Concentration”, and whether to use selected wells “INSIDE” or “OUTSIDE” the defined concentration range. QIAgility Version 4.18 User Manual 06/2023...
  • Page 85 5. To add selected wells to the existing sample bank, click on the Add Selection and Close button. To remove selected wells from the existing sample bank, click on the Delete Selection and Close button. QIAgility Version 4.18 User Manual 06/2023...

  • Page 86: View And Edit Information Using The Right-Hand Pane

    However, correct tip availability should always be checked before starting a run. Two tip types can be used with the QIAgility — 50 µL and 200 µL. In the software worktable, 50 µL tips are colored blue, and 200 µL tips are colored red.
  • Page 87 Enables mixing during dilution. dilution No. of times to mix Defines the number of times to mix diluent and standard. diluent and standard Change tips after each Instructs the instrument to change tips after each dilution. dilution? QIAgility Version 4.18 User Manual 06/2023...
  • Page 88 3. After entering each concentration, click on the OK tab to update the table. If desired, click on the Restore Defaults button to return to the original default concentrations. 4. Click on the OK button. QIAgility Version 4.18 User Manual 06/2023...

  • Page 89: Reagents

    To improve precision and accuracy, it is recommended that reagents are added to a master mix, rather than pipetted individually. When the per-reaction volume of reagent is <1 µL (i.e., outside the QIAgility pipetting range), the reagent must be added to a master mix.
  • Page 90 Enables a reagent to be defined as viscous. If a reagent is defined as viscous, a lower pipetting speed will be used. Mix count Defines the number of times the reagent will be mixed at the start of the run. This ensures that reagents are homogeneous. QIAgility Version 4.18 User Manual 06/2023...

  • Page 91: Master Mix

    Master Mix Master mixes can be prepared using the QIAgility, or a premade master mix can be used. Master mixes can be added to reaction wells. By default, master mix is pipetted into a reaction well first, because it normally constitutes the greatest volume of the reaction.
  • Page 92 Run settings menu. This setting is ignored when the mix is used in at least one reaction configured in the run. Wait for confirmation Select to wait for confirmation after adding the final component of the master mix. after adding final ingredient? QIAgility Version 4.18 User Manual 06/2023...
  • Page 93 Select to save the master mix setup. Enter a file name and a location, then click on the Save button. This option is useful when the master mix is used regularly, but the sample and reaction configuration changes. QIAgility Version 4.18 User Manual 06/2023...
  • Page 94 Use None Deselects all of the reagents for use in the master mix. All cells in the “Use?” column will be populated with No. Plate Defines the plate type that the reagent is located in. QIAgility Version 4.18 User Manual 06/2023...
  • Page 95 11. Click on the Close button. The corresponding well in the Master Mix Block will now be colored yellow. 12. To prepare another master mix, left-click on a different well in the Master Mix Block and repeat the above procedure. QIAgility Version 4.18 User Manual 06/2023...
  • Page 96 Setting up a premade master mix 1. Left-click on a well in the Master Mix Block. The “Mix” right-hand pane will appear. 2. Select Use Master Mix?, and then select “Use pre-mixed Master Mix?”. QIAgility Version 4.18 User Manual 06/2023...
  • Page 97 5. To reset the location of any reagent, double-click on a reagent in the list, then left-click and drag again. Alternatively, click on the Reset All button to clear all reagent locations, other than those that were defined upon entering the screen. 6. Click the OK button to continue. QIAgility Version 4.18 User Manual 06/2023...

  • Page 98: Samples

    Diluent can be used as a sample for nontemplate controls. Standards are treated in the same way as samples. Sample banks can be modified or created using the “Sample” right-hand pane. To view the “Sample” right-hand pane, left-click on a “Sample” well. QIAgility Version 4.18 User Manual 06/2023...
  • Page 99 Deletes any user-assigned sample names, sample IDs and concentration values. Sample IDs are reset to “blank”, and concentrations are reset to “0”. <select sample bank> A sample bank can be selected from the drop-down menu. QIAgility Version 4.18 User Manual 06/2023...
  • Page 100 Alternatively, it is possible to copy and paste a column of ”Yes”/”No” values from a spreadsheet. Right-click in the cell of the desired starting row, then select Paste to current cell. QIAgility Version 4.18 User Manual 06/2023...

  • Page 101: Reactions

    Delete Deletes selected reactions. To delete more than one reaction, hold down the “Shift” or “Ctrl” key, left-click on reactions in the “Reaction List” to select them, and then click on the Delete button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 102 This function is useful, for example, when it is convenient to dispense liquids by column (vertical pipetting order), but to export the data by row (horizontal label order). Copy all Select to copy the “Reaction Data” list. The data can be pasted into an Excel file. QIAgility Version 4.18 User Manual 06/2023...
  • Page 103 When a reaction contains more than one sample, no final concentration value can be calculated, so no value is displayed. If desired, the user can assign a concentration value to the reaction well. QIAgility Version 4.18 User Manual 06/2023...
  • Page 104 This function increases convenience when making a series of liquid handling operations in which the source wells for a given operation correspond to the target wells constructed by a predecessor operation. “Reaction Configuration” dialog box To open the “Reaction Configuration” dialog box, click on the Add button from the “Reaction” right-hand pane. QIAgility Version 4.18 User Manual 06/2023...
  • Page 105 An initial value is automatically set when the operation includes a master mix configured with default sample volume. A sample volume of zero is permitted. QIAgility Version 4.18 User Manual 06/2023...
  • Page 106 2. The “Reaction” right-hand pane will appear. 3. Click on the Add button. 4. Select a sample bank from the drop-down menu in the “Samples” panel. Note: Only one sample bank can be selected per reaction. QIAgility Version 4.18 User Manual 06/2023...
  • Page 107 Many of the “Special” operations allow the user to select the starting well. In these cases, there is a risk of mixing samples. A warning will be displayed at the start of the run if sample will be added to a well that already contains another sample. QIAgility Version 4.18 User Manual 06/2023...
  • Page 108 Enables the user to prepare a stock plate of normalized samples and a working plate using a minimal normalization number of tips. If a minimal number of tips is used, the sample plate will be contaminated with diluent. QIAgility Version 4.18 User Manual 06/2023...
  • Page 109 5. The “Configure Pause” dialog box will appear. 6. Adjust the pause time using the arrows (h:min:s). Note: The “Pause time” can be between 1 s and 96 h. 7. Click on the OK button. The reaction list will be updated. QIAgility Version 4.18 User Manual 06/2023...
  • Page 110 The three samples are then pipetted into the reaction plate. The second and third sample plates are therefore contaminated. In contrast, there is no sample plate contamination when using Add ‘reset eject position’. QIAgility Version 4.18 User Manual 06/2023...
  • Page 111 Defines a component sample bank to be added to the target reaction wells in a pooling step. Volume Defines the volume of each sample in the sample bank to be added during a pooling step. QIAgility Version 4.18 User Manual 06/2023...
  • Page 112 1. Left-click on a “Reaction” plate. 2. The “Reaction” right-hand pane will appear. 3. Click on the Special button. 4. Select Add sample bank duplication step. 5. The “Bank Duplication” dialog box will appear. QIAgility Version 4.18 User Manual 06/2023...
  • Page 113 10. Left-click on a reaction plate to select it. The “Bank Duplication” dialog box will reappear. Note: If there is only one “Reaction” plate, this plate will be selected by default. Left-click on the selected plate to return to the “Bank Duplication” dialog box. QIAgility Version 4.18 User Manual 06/2023...
  • Page 114 See Section 5.6.12 for more details about how to configure multi-eject settings. Sample Multi- Opens the “MULTI-EJECT” dialog box, which enables configuration of sample multi-eject options. eject Settings See Section 5.6.12 for more details about how to configure multi-eject options. QIAgility Version 4.18 User Manual 06/2023...
  • Page 115 1. Create a sample bank that contains 1 well. See Section 5.8.1 for details about how to do this. 2. Left-click on a “Reaction” plate. 3. The “Reaction” right-hand pane will appear. 4. Click on the Special button. 5. Select Add serial dilution step. QIAgility Version 4.18 User Manual 06/2023...
  • Page 116 Note: If there is only one “Reaction” plate, this plate will be selected by default. To return to the “Serial Dilution” dialog box, left-click on the selected plate. 10. Define the options as required in the “Special settings for Dilutions” panel. QIAgility Version 4.18 User Manual 06/2023...
  • Page 117 Defines the first well of the reaction plate into which the sample normalization will be targeted. Final volume Defines the final volume. Desired concentration Defines the concentration to normalize to. Mix count Defines the number of times to mix. QIAgility Version 4.18 User Manual 06/2023...
  • Page 118 1. Left-click on a “Reaction” plate. 2. The “Reaction” right-hand pane will appear. 3. Click on the Special button. 4. Select Add remove waste (pool bank members…). 5. The “Remove Waste” dialog box will open. QIAgility Version 4.18 User Manual 06/2023...
  • Page 119 11. Click on the OK button to return to the main software window. The remove waste command will be added to the “Reaction List”. Note: With this function, more than one sample will be added to one well. Therefore, a warning is displayed at the start of the run. QIAgility Version 4.18 User Manual 06/2023...

  • Page 120: Importing And Exporting Reaction And Sample Data

    5. Select either New Bank or Existing Bank. If New Bank is selected, enter a sample bank name. If Existing Bank is selected, left-click on a sample bank to select it. 6. Click on the OK button to return to the main software window. QIAgility Version 4.18 User Manual 06/2023...
  • Page 121 5. Some text-based spreadsheets contain strings of $, “, or # symbols. If these are not required as part of the sample names, select Remove from fields, separate with. Enter the symbol to be removed. Multiple character sequences can be removed by separating them with “;”. QIAgility Version 4.18 User Manual 06/2023...
  • Page 122 During the import, the software ensures that for each column in the list the corresponding sample bank is created or updated to include the member wells of that column in the data set imported. See Sections 5.8.1 and 5.9.5 for more information about how to manage sample banks. QIAgility Version 4.18 User Manual 06/2023...
  • Page 123 Sample and reaction data, which is listed in the “Sample” and “Reaction” right-hand pane, can be exported in a number of formats. This can be used to transfer data to the Rotor-Gene Q, or other data storage systems (e.g., LIMS environments). QIAgility Version 4.18 User Manual 06/2023...
  • Page 124 A *.csv file with format fixed as “well-location, plate identifier, sample identifier” (using the substitution pattern %2%0%P%0%I, as described in “Data fields” in this section). This describes the mapping of a tube/well location (plate and well label) to its contents (sample identification). QIAgility Version 4.18 User Manual 06/2023...
  • Page 125 A ‘Setup Table Format’ file which can be used with the ABI 7000/7500 SDS software absolute quantification v1.2.3 for absolute quantification. Master mixes must be named in the QIAgility software according to special conventions which allow the identification of Detector, Reporter, Quencher entities required by the ABI SDS software.
  • Page 126 (label or pipet) over the whole plate. 5. Click on the Export button. 6. Enter a file name and select the location to which the data will be exported. 7. Click on the Save button to continue. QIAgility Version 4.18 User Manual 06/2023...
  • Page 127 The %0 between the individual data fields indicates that a separator is to be inserted. A separator will result in a comma in the case of a *.csv file. In an Excel spreadsheet, a column break would be inserted. QIAgility Version 4.18 User Manual 06/2023...

  • Page 128: Progress Report

    Progress report When a run is in progress, the status of the run is summarized in the right-hand pane. QIAgility Version 4.18 User Manual 06/2023...

  • Page 129: Programming Run Files

    To set up a PCR reaction, using the “PCR Setup Wizard”: 1. Select Wizard/PCR Setup Wizard, or select PCR Setup Wizard from the splash screen that appears when the software is started. 2. The QIAgility Setup Wizard will appear. QIAgility Version 4.18 User Manual 06/2023...
  • Page 130 7. Left-click on a plate to select it. Click on the Set Function button. A submenu will appear. To update the plate function, select Sample, Reaction, Reagent, Standard, or Mix. 8. Repeat step 7 for all plates on the worktable. 9. Click on the Next button to continue. QIAgility Version 4.18 User Manual 06/2023...
  • Page 131 “Viscous?” column, check the box, and then click on the OK tab. Repeat this procedure to change a reagent from non-viscous to viscous. 12. Click on the OK button to continue. Repeat this step until all reagent segments you want to use are defined. QIAgility Version 4.18 User Manual 06/2023...
  • Page 132 16. Click on the OK button to continue. 17. The “Standards Editor” screen will appear. (steps 17 to 21 will only appear if you selected “I want to use standards in this run” on the first page of the wizard.) QIAgility Version 4.18 User Manual 06/2023...
  • Page 133 19. Define the “Number of dilution concentrations”, the “Concentration of undiluted standard”, and the “Dilution ratio”. 20. Click on the OK button to continue. 21. Repeat steps 18–20 for any remaining standards. 22. Click on the Next button to continue. QIAgility Version 4.18 User Manual 06/2023...
  • Page 134 25. Click on the Next button to continue. 26. The “Reaction Editor” screen will appear. Master mix Defines the master mix that will be used in a reaction. Standards/-samples Defines the standard or sample that will be used in a reaction. QIAgility Version 4.18 User Manual 06/2023...

  • Page 135: Optimisation Wizard

    This section outlines the Optimisation Wizard screens that will guide the user through the setup. Instructions are displayed on each screen. Note: The Optimisation Wizard only provides steps to set up an optimized matrix, it does not provide steps for preparation of a master mix. QIAgility Version 4.18 User Manual 06/2023...
  • Page 136 Defines the volume of reagent to be used in the reaction. reagent would you like to use in the final reaction? How many different Defines the number of different concentrations of reagent to be optimized. concentrations would you like to use? QIAgility Version 4.18 User Manual 06/2023...
  • Page 137 14. If only one reagent will be optimized (i.e., if option 1 was selected in step 3), proceed to step 15. If more than one reagent will be optimized (i.e., option 2 or 3 was selected in step 3), repeat steps 7–13, and then proceed to step 15. QIAgility Version 4.18 User Manual 06/2023...
  • Page 138 17. Define the master mix to be used in the reaction. 18. Click on the Create Premixed MM button. The “Plate/well Selector” window will appear. 19. Left-click on a well in the Master Mix Block to select the location of the master mix. QIAgility Version 4.18 User Manual 06/2023...

  • Page 139: Using "Empty Project

    5. Set the tip availability for every plate that has Tip function. See Section 5.7.2 for details about setting tip availability. 6. Configure the samples, diluent, standards, reagents and master mix. See Section 5.9. QIAgility Version 4.18 User Manual 06/2023...

  • Page 140: Example Reactions

    Master mix with standard 1. Select the dilution series from the “Samples” panel. 2. Select the master mix. 3. Enter the number of replicates in the “Target wells” panel. 4. Click on the OK button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 141 No template control with master mix 1. Select “Diluent” from the “Samples” panel. 2. Select the master mix. 3. Enter the number of replicates in the “Target wells panel”. 4. Click on the OK button. QIAgility Version 4.18 User Manual 06/2023...

  • Page 142: Checking The Reaction Setup

    Alternatively, position the cursor above a source well (i.e., diluent, reagent, master mix, or standard). A hover box will appear that reports the volume required. This volume includes an extra volume, to account for the dead volume, that is dependent on the tube type. QIAgility Version 4.18 User Manual 06/2023...

  • Page 143: Selecting The Com Port

    Note: The software lists only eligible COM ports and marks the mostly likely port on which the instrument can be found with an asterisk (*). For correct communication of the laptop (supplied with the QIAgility) with the instrument: 4. The following message will appear.
  • Page 144 7. The service that has been acknowledged will now be colored black in the “Service Register” dialog box. In addition, the component will be colored in green, as a reminder of a future service. Note: Once a service has been acknowledged and saved, it cannot be edited. QIAgility Version 4.18 User Manual 06/2023...
  • Page 145 Note: After acknowledgment of a pipettor service, volume calibration must be done before the start of the first run. An error is shown at the start of a run if no volume calibration has been done. QIAgility Version 4.18 User Manual 06/2023...

  • Page 146: Operating Procedures

    Before proceeding, we recommend that you familiarize yourself with the features of the QIAgility by referring to Sections 3.2 and 3.2.10. The QIAgility lid must remain closed during operation of the instrument. Only open the lid when the instrument is not operating or when instructed to do so by the software.
  • Page 147 Open button. Alternatively, double-click on the run file to open it directly. 1. The main software window will open. See Section 5.3 for a description of the features of the main software window. QIAgility Version 4.18 User Manual 06/2023...

  • Page 148: Using The Uv Lamp

    Enables the user to set UV lamp exposure times of between 1 second and 30 minutes, using the arrows. Start Initiates UV lamp operation. Close software when Shuts down the QIAgility Software when UV lamp operation is complete. UV finishes? Enable Lamp Switches the UV lamp on. Disable Lamp Switches the UV lamp off.

  • Page 149: Operating The Hepa Filter

    Q Protocols Q Protocols are run files which have been developed to directly facilitate specific PCR applications. Q Protocols are installed within the QProtocols subfolder. To access this folder, select Installation directory of QIAgility Software\QIAgility\QProtocols (e.g., C:\Program Files\QIAgility\QProtocols). Q Protocols are locked. Edit access is only permitted to update the lab notebook, set tip availability, modify sample banks and assign plate IDs/barcodes and well IDs/barcodes to sample/reaction wells defined in the run.
  • Page 150 3. The welcome dialog will open. 4. Select the Protocols tab. All Q Protocols that are saved in the appropriate folder (i.e., QProtocols) will be displayed. 5. Select the desired Q Protocol. QIAgility Version 4.18 User Manual 06/2023...

  • Page 151: Selecting A Saved Run File

    5.9.7) and assign well IDs/barcodes to sample/reaction wells (see Sections 5.9.5, 5.9.6, and 5.9.7). Selecting a saved run file To open a saved run file: 1. Select File > Open, or click on the icon. 2. Select a file and then click on the Open button. QIAgility Version 4.18 User Manual 06/2023...

  • Page 152: Creating A New Run File

    See Sections 5.10.1, 5.10.2, and 5.10.3 for information about how to create a new run file, either using a wizard, or using the Empty Project” (last used layout) or Empty Project (default layout) function. QIAgility Version 4.18 User Manual 06/2023...

  • Page 153: Preparing The Worktable

    This volume includes the extra volume which allows for liquid recovery limitations and losses due to evaporation and tip contact during pipetting. Alternatively, view the pre-run report. 8. Close the instrument lid. QIAgility Version 4.18 User Manual 06/2023...

  • Page 154: Starting A Run

    2. The instrument will complete the current operation and will then pause. The following dialog box will appear. 3. Click on the Continue Run button to continue the run, or click on the Abort Run button to abort the run. QIAgility Version 4.18 User Manual 06/2023...

  • Page 155: Exception Handling

    2. Select the Tip plate where the tips have been replaced by checking the box next to the correct plate. 3. Click on the OK button to continue the run. Note: If the Cancel button is selected, the run will be aborted. QIAgility Version 4.18 User Manual 06/2023...

  • Page 156: Reports/Log Files

    1. Open the run file for which the pre-run report will be generated. 2. If necessary, adjust the run file according to your needs. 3. Select Wizards/Generate report. The pre-run report will be displayed. QIAgility Version 4.18 User Manual 06/2023...

  • Page 157: Post-Run Report

    Run duration  Exceptions: Date and time of exception  Instrument activity at time of exception  Details of the exception  User response  Note: Any exceptions are highlighted in the post-run report. QIAgility Version 4.18 User Manual 06/2023...

  • Page 158: Log Files And Support Packages

    A new log file is generated when the software starts, when a run starts, or when a log file reaches 16 MB in size. The 8 most recent logs are stored. Log files are saved under “selected installation directory”/Data/Logs (e.g., C:\Program Files\QIAgility\Data\Logs). To view a log file: 1.
  • Page 159 Note: On completion of each run, the software automatically saves a support package. However, for problems that occur outside the context of a run it is important to manually create the support package immediately after a suspected error has occurred. Support packages are important when reporting a problem to QIAGEN Technical Services. To create a support package: 1.

  • Page 160: Importing And Exporting Reaction/Sample Data

    Sample and reaction data can be imported and exported. See Section 5.9.7. Extraction data from earlier purifications can be imported and used as sample data. In addition, data can be exported for use in downstream applications, for example with the Rotor-Gene Q. QIAgility Version 4.18 User Manual 06/2023...

  • Page 161: Shutting Down The Software And The Instrument

    (recommended) was selected, the robot will discard the tip, and the pipetting head will now move to a safe location. The following message will appear. 13. Click on the OK button. 14. Set the power switch of the QIAgility to the off position. QIAgility Version 4.18 User Manual 06/2023...
  • Page 162 If the head holds a tip it will not be discarded. As the instrument is switched off, the pipetting head will drop approximately 70 mm. This will not damage the instrument but may result in a pipet tip being picked up. QIAgility Version 4.18 User Manual 06/2023...

  • Page 163: Instrument Setup And Calibration

    Before starting any runs, the QIAgility must be calibrated. This section outlines the calibrations that must be performed. If the QIAgility is moved, we recommend recalibrating the plate positions. Unless the pipetting mechanism has been serviced, or pipetted volumes are found to be inaccurate, there is no need to recalibrate the volume.
  • Page 164 10. If the pipetting hub is not correctly located, click on the No button. The following dialog box will appear. 11. Manually adjust the X- and Y-axis positions, using the arrows, and then click on the Locate Z Axis button. 12. The following message box will appear. QIAgility Version 4.18 User Manual 06/2023...
  • Page 165 Before starting the procedure: Open the instrument lid.  Place plates to be calibrated onto the worktable.  Ensure that 200 µL tips are available in at least one Tip Rack Holder.  QIAgility Version 4.18 User Manual 06/2023...
  • Page 166 7. Click on the Save button. The current position is now saved as the calibrated value. If desired, click on the Check Pos button to confirm that the correct position has been saved. 8. To perform position calibrations on other plates, repeat steps 3–7. 9. When position calibrations are complete, click on the Close button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 167 Abort Aborts the current calibration activity. Manual Setup Initiates the manual plate height calibration operation. Manual plate height calibration is (highlighted items) performed only for the selected plate. QIAgility Version 4.18 User Manual 06/2023...
  • Page 168 10. If more than one plate was selected in the “Plates to Calibrate” list, the instrument will calibrate each plate in turn. Repeat steps 6–9 for each plate. 11. When all calibrations are complete, click on the Close button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 169 5–7, starting with a lower “Level at bottom of well” value to avoid driving the tip forcefully into the base of the well. 9. Click on the OK button when the correct height calibration has been set. 10. Click on the Cancel button when all calibrations are complete. QIAgility Version 4.18 User Manual 06/2023...
  • Page 170 Place a tube in the first well of the reaction plate.  Ensure that 50 µL and 200 µL tips are available, that Tip Rack Holders are calibrated and that correct tips are set as  available. QIAgility Version 4.18 User Manual 06/2023...
  • Page 171 5. Ensure that the correct tip type is selected in the “Select tip types to profile” panel. 6. Define the number of tips to be used to calculate the average offset. 7. Click on the Start button to start tip offset calibration. QIAgility Version 4.18 User Manual 06/2023...
  • Page 172 “Option 1” from the “Volume Calibration Wizard”. Note: Volume calibration must be done before the start of the first run on a newly installed QIAgility. An error is shown at the start of the run if no volume calibration has been done.
  • Page 173 3. Click in the appropriate cell in the “Value (P axis units)” column to manually change the P-axis units. 4. Click on the Next button. 5. Click on the Yes button to continue. 6. Click on the OK button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 174 11. Click on the Close button to return to the “Volume Calibration Wizard — History of Calibration Data” dialog box. 12. Click on the Finish button to return to the main software window. QIAgility Version 4.18 User Manual 06/2023...
  • Page 175 2. The following message will appear. 3. Click on the Yes button. 4. Click on the Cancel button in the “Volume Calibration” dialog box. The volume calibrations will now be updated with the factory default calibration settings. QIAgility Version 4.18 User Manual 06/2023...
  • Page 176 Only perform maintenance that is specifically described in this user manual. Servicing The QIAgility is supplied with a warranty that lasts for 1 year, beginning on the date of shipment. The warranty includes all repairs due to mechanical breakdown. Application development, software upgrades, accessories, and disposable items are not included in the warranty.
  • Page 177 Regular maintenance procedures Cleaning the QIAgility CAUTION Damage to the instrument Do not use solvents, or reagents containing acids, alkalis, or abrasives to clean the QIAgility. WARNING Risk of electric shock Do not open any panels on the QIAgility. Risk of personal injury and material damage Only perform maintenance that is specifically described in this user manual.
  • Page 178 Periodic maintenance Decontaminating the QIAgility To remove DNA/RNA from the worktable and to eliminate cross-contamination, the QIAgility must be routinely decontaminated. Proceed as follows: 1. Remove all loading blocks and the tip ejector chute from the worktable. Wash these in detergent and rinse with clean water.
  • Page 179 3. Using the chain, pull out the sliding security bolt. 4. Remove the tip ejector/tip ejector chute. Replacing the tip ejector chute 1. Open the lid. 2. Replace the tip ejector chute. 3. Press the sliding security bolt into the side slot opening. QIAgility Version 4.18 User Manual 06/2023...
  • Page 180 1. Lift the HEPA filter lid section. 2. Remove the lid. 3. Unscrew the 4 thumb screws. 4. Remove the “H” Frame. 5. Remove the HEPA filters. 6. Replace the HEPA filters and reassemble. QIAgility Version 4.18 User Manual 06/2023...
  • Page 181 QIAgility Software does not launch QIAgility Software not installed Install the QIAgility Software. See Section 4.6. Old version of Microsoft Windows The QIAgility Software operates with Windows XP Professional or Windows 7. See Section 3.4. Operation Comments and suggestions Running out of master mix Master mix remains in the tube after Ensure that the height calibration of the master mix tube is set correctly.
  • Page 182 Instrument pipets in mid-air or pipets no liquid Level sensor malfunction Static electricity on the plasticware may be interfering with operation of the liquid-level sensor. Generate a support package and contact QIAGEN Technical Services. QIAgility Version 4.18 User Manual 06/2023...
  • Page 183 Software does not start and the “A required library file is not correctly installed or registered” error message is displayed Present version of the software conflicts with Reinstall the most recent version of the QIAgility software. an older version of the software Software running incorrectly with a “Software error: aborting”...
  • Page 184 The run can commence with active warning messages, but this may lead to erroneous results. Note: Warnings and errors are logged in the post-run report and in any support packages. QIAgility Version 4.18 User Manual 06/2023...
  • Page 185 The correct tips for the required volume are not available. Volume calibration must be done before run Volume calibration must be done before a run can be started in these situations: New installation of instrument After pipettor service QIAgility Version 4.18 User Manual 06/2023...
  • Page 186 All instrument errors are associated with an error number. All error numbers start with 40000. If an instrument error occurs, immediately save a support package and contact QIAGEN Technical Services. See Section 6.7.3 for details about how to save a support package.
  • Page 187 Close Closes the dialog box. Go Home Homes all axes and resets the positional reference for the instrument for subsequent movements including tip ejection. Clear Messages Clears the content of the messages text box. QIAgility Version 4.18 User Manual 06/2023...
  • Page 188 Mechanical tests Check Home-Opto Alignment This test ensures that the optical homes in X-, Y- and possibly Z-axes in QIAgility instruments are 3, 4, and 5 steps away from their L297 software home, respectively. Check for loss of steps This test establishes whether the instrument has reliable movement. The default iteration setting of 5 will take approximately 20–...
  • Page 189 –25°C to 60°C (–13°F to 140°F) in manufacturer’s packaging Relative humidity Max. 75% (noncondensing) Storage conditions Air temperature 15–30°C (59–86°F) in manufacturer’s packaging Relative humidity Max. 75% (noncondensing) * Conditions under which equipment is electrically safe to operate. QIAgility Version 4.18 User Manual 06/2023...
  • Page 190 41 kg (90.2 lb.) standard configuration Capacity Up to 96 samples per run Software QIAgility Software is supplied with the QIAgility System. Default protocols are available for use with the QIAgility Software and can be downloaded from www.qiagen.com/goto/QIAgility. QIAgility Version 4.18 User Manual 06/2023...
  • Page 191 Power switch A button located at the back of the QIAgility. It allows the user to switch the QIAgility on and off. Q Protocol Q Protocols are run files which have been developed by QIAGEN to directly facilitate specific PCR applications. Q Protocols are installed in the “QProtocols”...
  • Page 192 For absolute quantification, the ABI software requires each reaction to be associated with a detector, reporter and quencher. To achieve this, master mixes used in the exported reactions must be named in the QIAgility software according to the convention: [?:]D_R_Q{:D_R_Q} Indicates a string of characters, which excludes “:”.
  • Page 193 In the resultant files, wells are only listed where their master mix contents are named, so that they can match the required format. Advanced settings Advanced run settings are available to adjust specific applications on the QIAgility. These functions should be modified only under direct instructions from QIAGEN. Tip pickup mode This defines the strategy used for collecting tips from the Tip Rack Holder.
  • Page 194 A tick is displayed when this function is activated. By default, all of the liquid handling functions available via the Special button, presented in the “Reaction” right-hand pane, work only with volumes up to the largest capacity tip supported on the QIAgility (i.e., 200 µL).
  • Page 195 False triggering which occurs within the tube is undetectable. QIAgility Version 4.18 User Manual 06/2023...
  • Page 196 This function is activated by the ‘use optimal height calibration where possible’ advanced setting. Before starting the procedure: Ensure that 50 µL tips are available, that the Tip Rack Holder is calibrated, and that the correct tips are set as available.  QIAgility Version 4.18 User Manual 06/2023...
  • Page 197 5. Set the number of wells to measure in the Take average of how many wells? box. By default, this value is 6. Click on the Autodetect (checked items) button to initiate calibration. QIAgility Version 4.18 User Manual 06/2023...
  • Page 198 This process is repeated for each vessel to be measured. Where the segment is not eligible for optimal height calibration, a single tip is used to probe the base of the vessel and the process proceeds as described in Section 7.1.3, “Automatic plate height calibration”. QIAgility Version 4.18 User Manual 06/2023...
  • Page 199 10. If more than one plate was selected in the “Plates to Calibrate” list, the instrument will calibrate each plate in turn. Repeat steps 8 and 9 for each plate. 11. When all calibrations are complete, click the Close button. QIAgility Version 4.18 User Manual 06/2023...
  • Page 200 <Contents> <Diluent> <SocketName>M1</SocketName> <WellLabel>A</WellLabel> <Vol>40</Vol> </Diluent> <Mix> <SocketName>M1</SocketName> <WellLabel>B</WellLabel> <Vol>10</Vol> <Name>Mix B</Name> </Mix> <Reagent> <SocketName>R1</SocketName> <WellLabel>I</WellLabel> <Vol>2.5</Vol> <Name>10X Buffer</Name> </Reagent> <Sample> <SocketName>C1</SocketName> <PlateID/> <WellLabel>A1</WellLabel> <Vol>20</Vol> <Name/> <SampleID/> <Concentration>0</Concentration> </Sample> <Reaction> <SocketName>R1</SocketName> <WellLabel>I</WellLabel> <Vol>40</Vol> <Concentration>74</Concentration> QIAgility Version 4.18 User Manual 06/2023...
  • Page 201 SL_FullID = 0 SL_SampleID = 1 SL_ReactionID = 2 SL_SampleName = 3 EmptyLabelType Possible values:  EL_Empty = 0 EL_WellID = 1 EL_WellOrder = 2 EL_WellNumber = 3 They can be evaluated during the transformation. QIAgility Version 4.18 User Manual 06/2023...
  • Page 202 Risk of personal injury and material damage CAUTION The QIAgility is too heavy to be lifted by one person. To avoid personal injury or damage to the instrument, do not lift the instrument alone. If you need to transport the QIAgility, package the instrument as follows: 1.
  • Page 203 Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE- marked electronic equipment is provided.
  • Page 204 Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.
  • Page 205 QIAgility HEPA/UV (w/o PC) System* Robotic workstation for automated PCR setup (with 9001908 UV light and HEPA filter); comes with QIAgility software; includes installation, application training, and 1 year warranty on labor, travel, and parts. Does not include a laptop.
  • Page 206 Reagent Block, 16 x 0.2 mL/2 mL Flat- Reagent Block; intended to hold reagents and 9018953 Base Tubes standards on the instrument worktable; holds 16 x 0.2 mL PCR tubes and 16 x 2 mL Flat-Base Tubes QIAgility Version 4.18 User Manual 06/2023...
  • Page 207 Replacement cartridge (one filter) 9019486 * Additional instrument and Service bundles are available. For up-to-date licensing and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.
  • Page 208 Appendix D – Consignes de sécurité Avant d’utiliser le QIAgility, il est impératif de lire attentivement ce manuel et de porter une attention particulière aux consignes de sécurité. Afin de garantir un fonctionnement de l’appareil en toute sécurité et de maintenir l’appareil en bon état de marche, il est impératif de suivre les instructions et consignes de sécurité...
  • Page 209 Ne jamais actionner ou cliquer sur aucunes touches tant que des parties du corps se trouvent dans l’enceinte de l’appareil. En cas d’urgence, éteindre le QIAgility à l’aide de l’interrupteur d’alimentation situé à l’arrière de l’appareil et débrancher le cordon d’alimentation de la prise de courant.
  • Page 210 Afin que le QIAgility fonctionne de manière satisfaisante et en toute sécurité, suivre les conseils suivants : Le cordon d’alimentation de l’appareil doit être branché sur une prise d’alimentation munie d’un conducteur de protection  (terre/masse). Ne pas régler ni remplacer les pièces internes à l’instrument.
  • Page 211 AVERTISSEMENT Pièces mobiles Éviter le contact avec les pièces en mouvement lorsque le QIAgility est en fonctionnement. Ne jamais placer les mains sous le bras robotisé lorsque celui-ci s'abaisse. Ne pas tenter de déplacer des plaques ou des tubes lorsque le bras robotisé est en marche.
  • Page 212 Avant de retirer des plaques ou des tubes de la table de travail, sélectionner « Pause » dans la barre d’outils du logiciel  QIAgility pour mettre l’appareil en mode de pause. Attendre l’arrêt complet du bras robotisé. Cela peut prendre jusqu’à 10 s.
  • Page 213 N17965) Plaque signalétique à l’arrière de l’appareil Label RoHS pour la Chine (restriction de l’utilisation de certaines substances dangereuses dans l’équipement électrique et électronique) Plaque signalétique à l’arrière de l’appareil Lire le manuel d’utilisation. QIAgility Version 4.18 User Manual 06/2023...
  • Page 214 Appendix E – Sicherheitshinweise Lesen Sie dieses Handbuch sorgfältig durch, bevor Sie den QIAgility benutzen. Beachten Sie dabei insbesondere die Sicherheitshinweise. Die Gebrauchsanweisungen und Sicherheitshinweise im Handbuch müssen befolgt werden, um einen sicheren Betrieb des Geräts zu gewährleisten und das Gerät in einem sicheren Zustand zu erhalten.
  • Page 215 Sie nicht auf die Maus und drücken Sie nicht auf eine Taste der Tastatur, während sich Ihre Hände oder andere Körperteile im Arbeitsbereich des Roboterarms befinden. Schalten Sie im Notfall den QIAgility aus (der Netzschalter befindet sich auf der Geräterückseite), und ziehen Sie den Netzstecker aus der Steckdose.
  • Page 216 UV-Lampe eingeschaltet werden kann. Der Hauben Sensor darf unter keinen Umständen ausser Kraft gesetzt werden. Um einen zufriedenstellenden und sicheren Betrieb des QIAgility zu gewährleisten, befolgenden Sie bitte die nachstehenden Richtlinien: Das Netzkabel muss an eine Wechselstrom-Steckdose mit Schutzleiter (Erdungs-/Masseleiter) angeschlossen werden.
  • Page 217 COSHH: Control of Substances Hazardous to Health (Vereinigtes Königreich) ‡ Giftige Dämpfe Alle Arbeiten mit flüchtigen Lösungsmitteln oder toxischen Substanzen müssen unter einem funktionierenden Laborabzugssystem durchgeführt werden, damit die möglicherweise entstehenden Dämpfe abziehen können. QIAgility Version 4.18 User Manual 06/2023...
  • Page 218 Stellen Sie keine Gegenstände auf den Roboterarm.  Wählen Sie die Option “Pause” in der Werkzeugleiste der QIAgility Software, um das Gerät anzuhalten, bevor Sie  Reaktionsgefäße oder Platten von der Arbeitsplattform entfernen. Warten Sie, bis der Roboterarm die gerade ablaufende Bewegung beendet hat –...
  • Page 219 ACHTUNG Beschädigung der QIAgility Gerätehaube Verwenden Sie weder Alkohol noch Lösungsmittel oder Reagenzien, die Säuren, Laugen oder Abrasivstoffe enthalten, um die QIAgility Haube zu reinigen. Alkohol schädigt die Gerätehaube. Verwenden Sie zum Reinigen der Gerätehaube ausschließlich destilliertes Wasser. ACHTUNG Beschädigung der QIAgility Seitenwände Reinigung sie niemals die Gerätehaube und Seitenwände mit Alkohol oder alkoholhaltigen Lösungen.
  • Page 220 Communications Commission Vereinigten Staaten Typenschild auf der Geräterückseite RCM-Zeichen (ehemals C-Tick) für Australien (Anbieterkennzeichnung N17965) Typenschild auf der Geräterückseite Markierung gemäß RoHS-Richtlinie für China (Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro- und Elektronikgeräten) QIAgility Version 4.18 User Manual 06/2023...
  • Page 221 Document Revision History Revision Description June 2023 Included the UKCA symbol. Removed the environmental class. QIAgility Version 4.18 User Manual 06/2023...
  • Page 222 (E.I. du Pont de Nemours and Company). Copyright Information The QIAgility software uses an XSLT template for scientific number to decimal conversion provided by Ludek Vodicka under MIT license. Copyright © 2010 Ludek Vodicka Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.
  • Page 223 Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com QIAgility Version 4.18 User Manual 06/2023...

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