Qiagen DML 3000 User Manual
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Qiagen DML 3000 User Manual

For use with the digene hybrid capture 2 dna tests
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DML 3000 User Manual
For use with the
5000-00031
QIAGEN
19300 Germantown Road
Germantown, MD 20874
USA
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
L01052 Rev. 7
®
digene
Hybrid Capture
Sample & Assay Technologies
®
2 DNA tests

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  • Page 1 DML 3000 User Manual ® ® digene For use with the Hybrid Capture 2 DNA tests 5000-00031 QIAGEN 19300 Germantown Road Germantown, MD 20874 QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden GERMANY L01052 Rev. 7 Sample & Assay Technologies...
  • Page 2 The LumiCheck Plate, its components, or its method of use may be covered by the following patent and its international counterpart: U.S. Patent No. 6,335,997 Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. © 2013-2015 QIAGEN, all rights reserved.

  • Page 3: Table Of Contents

    Plate carrier Plate mask Plate mask release latch 3-10 Microplate locator 3-10 3.10 Detector 3-12 3.10.1 Background signal 3-12 3.10.2 Efficiency 3-12 3.10.3 Sensitivity 3-12 3.10.4 Dynamic range 3-12 3.10.5 Linearity 3-13 3.10.6 Spectral response 3-13 DML 3000 User Manual Contents-1...
  • Page 4 Installation Connecting the components General Operation Moving the transport locks to the home position Placing a microplate in the DML 3000 Securing the DML 3000 using the transport locks Transporting the DML 3000 Maintenance Cleaning the DML 3000 7.1.1 Cleaning the exterior of the DML 3000 7.1.2...
  • Page 5 Contents FCC Declaration Index Index-1 DML 3000 User Manual Contents-3...

  • Page 6: Dml 3000 User Manual

    Contents This page intentionally left blank Contents-4 DML 3000 User Manual...

  • Page 7: Introduction

    Please contact QIAGEN Technical Services. Intended use of the instrument The DML 3000 is intended to measure light that is emitted as a result of a chemiluminescent reaction. Assay results obtained using chemiluminescence technology in a 96-well microplates are calculated and interpreted according to assay validation parameters.

  • Page 8: Requirements For Instrument Users

    Maintenance Laboratory Appropriately trained, technicians or experienced, and familiar with the equivalent use of computers and automation in general Service QIAGEN Field Trained, certified, and authorized Service employees by QIAGEN or personnel trained by QIAGEN DML 3000 User Manual...

  • Page 9: Safety

    WARNING/ Risk of personal injury and material damage. CAUTION Improper use of the DML 3000 may cause personal injuries to the user or damage to the instrument. The DML 3000 must only be operated by qualified personnel who have been appropriately trained.
  • Page 10 WARNING Risk of personal injury Before lifting the DML 3000, position yourself at the back of the DML 3000. The weight distribution of the DML 3000 is heavier at the back than at the front. To prevent injury, bend your knees and lift the DML 3000 slowly to compensate for this weight difference.

  • Page 11: Electrical Safety

    DML 3000. For best performance of the DML 3000, plug the DML 3000 into a surge protector or uninterruptible power supply (UPS) before plugging it into a grounded power source. Environment...

  • Page 12: Biological Safety

    Vent for fumes and dispose of wastes in accordance with all national and local health and safety regulations and laws. Mechanical hazards WARNING Risk of personal injury Keep hands free of moving parts. Do not reach into the DML 3000 while it is operating. DML 3000 User Manual...

  • Page 13: Symbols On The Instrument

    CAUTION Damage to the instrument If the DML 3000 is powered ON, the optical head must remain in the home position while the access door is open. Attempting to move the optical head when the DML 3000 is powered ON exposes the optical head to ambient light.

  • Page 14: Additional Symbols Used In This User Manual

    Type plate on the back of Legal manufacturer the instrument On the back of the Consult instructions for use instrument Additional symbols used in this user manual Symbol Description Catalog number Authorized representative in the European Community DML 3000 User Manual...

  • Page 15: Functional Description

    Functional Description Functional Description The DML 3000 is highly sensitive with a broad dynamic range. It features a low background photon-counting PMT and measures light in the visible spectrum (350– 650 nm). Microplates are loaded at the front of the DML 3000. The individual microplate wells are sequentially moved beneath the opening of the detector and then individually measured.
  • Page 16 Functional Description The following is the front view of the DML 3000: DML 3000 User Manual...

  • Page 17: Led Status Lights

    DML 3000: red, yellow, and green. All three lights illuminate briefly after powering ON the DML 3000. The red LED status light indicates the DML 3000 is not operational. The yellow LED status light remains lit, unless the DML 3000 is performing a function as directed by the software.

  • Page 18: Access Door

    Functional Description Access door The access door is on the top of the DML 3000. It allows access to the plate mask and plate carrier. To open the door, lift the lower front edge. Example of opening the access door:...

  • Page 19: Gas Actuator Lift Arm

    Gas actuator lift arm The gas actuator lift arm supports the access door when it is opened and allows the access door to close evenly and smoothly. View of the gas actuator lift arm: Gas actuator lift arm DML 3000 User Manual...

  • Page 20: Optical Sensor

    Functional Description Optical sensor An optical sensor in the front-center of the DML 3000 measures if the door is open or closed. View of the access door opened with the optical sensor in view: Optical sensor Flag DML 3000 User Manual...

  • Page 21: Optical Head

    The optical head measures the chemiluminescence of each microplate well. The optical head has an optical mask and an optical mask retainer. The optical mask isolates one microplate well from the other microplate wells so that the DML 3000 DML 3000 User Manual...

  • Page 22: Plate Carrier

    Optical mask Plate carrier When a 96-well microplate is placed in the DML 3000 for measurement, the plate carrier keeps the microplate positioned securely against the plate mask. The spring- loaded base of the plate carrier pushes the microplate up into the plate mask for a tight fit.

  • Page 23: Plate Mask

    The plate mask is a metal cover with 96 holes aligned to the wells of a microplate. The plate mask is designed to minimize cross-talk between microplate wells. A view of the opened access door with the plate mask: Plate mask DML 3000 User Manual...

  • Page 24: Plate Mask Release Latch

    Plate mask release latch Microplate locator The back-right corner of the plate carrier has a microplate locator that retracts when the plate mask is open to allow the microplate to be placed in the plate carrier. When 3-10 DML 3000 User Manual...
  • Page 25 Functional Description the plate mask is closed, the microplate locator aligns the microplate properly in the X-axis and Y-axis. View of the microplate locator: Microplate locator A1 orienting corner DML 3000 User Manual 3-11...

  • Page 26: Detector

    3.10.4 Dynamic range Dynamic range is the relationship between the lowest and the highest signal that the detector is able to quantify. The dynamic range for the DML 3000 is 10 to 5x10 RLU. 3-12 DML 3000 User Manual...

  • Page 27: Linearity

    The transport locks are used to secure the internal components of the DML 3000 when the instrument is being transported. The DML 3000 is shipped from QIAGEN with the transport locks in the home position. The foam-block insert secures the plate carrier and optical head during transport. The...
  • Page 28 If the transport locks are not in the home position, see “Moving the transport locks to the home position,” page 6-1, for additional instructions. View of transport locks in the home position: Transport locks 3-14 DML 3000 User Manual...
  • Page 29 Functional Description The transport locks must be used prior to transporting the DML 3000. See “Securing the DML 3000 using the transport locks,” page 6-4, for additional instructions. View of the transport locks in the secured position and ready for transport:...

  • Page 30: Power Connection Port And Power Switch

    Functional Description 3.12 Power connection port and power switch Located at the back-left of the DML 3000, the power connection port is where the power adapter plugs into the DML 3000. The power switch powers the DML 3000 ON and OFF.

  • Page 31: Power Cord

    Power cord The power cord connects to the power source at one end and the power adapter at the other. For the best performance of the DML 3000, connect the power cord to a surge protector or an UPS. View of the power cord:...

  • Page 32: Power Adapter

    3.14 Power adapter The power adapter connects to the DML 3000 power connection port at one end and to the power cord at the other end. Important: Only use the power adapter provided with the DML 3000. Use of a power adapter other than the one provided with the DML 3000 voids the warranty.
  • Page 33 Functional Description the DML 3000. The USB-to-Serial converter is optional and supplied with the Personal Computer Country Kit. View of the USB-to-Serial converter: DML 3000 User Manual 3-19...
  • Page 34 Functional Description This page intentionally left blank 3-20 DML 3000 User Manual...

  • Page 35: Unpacking Procedures

    The DML 3000 must be unpacked from its shipping container. Place the box on an even surface. One person should be able to unpack the DML 3000 and lift it from the packing box. Note: Keep the original shipping bag, box, and foam inserts for future shipping requirements.
  • Page 36 Unpacking Procedures Bend your knees and lift the DML 3000 and packing foam out of the box. Example of removing the DML 3000 from the shipping box: Set the DML 3000 on a level surface. Remove the packing foam from either side of the DML 3000.
  • Page 37 View of the DML 3000 in plastic shipping bag: Remove the tape on the plastic shipping bag. Remove the DML 3000 from the plastic shipping bag, discard the 2 desiccant bags in the plastic shipping bag, and place the plastic shipping bag into the shipping box.
  • Page 38 Locate the foam-block insert on the right side of the DML 3000. View of foam-block insert in DML 3000: Foam-block insert Remove the foam-block insert from the DML 3000, and place it in the shipping box. Make sure that the transport locks are in the home position. See “Transport locks,”...

  • Page 39: Installation

    For power requirements and technical data, see “Appendix A,” page A-1 The DML 3000 type plate is located on the right side of the back panel next to the power switch. The type plate contains the DML 3000 serial number which is required...
  • Page 40 Note: The power connection port is located on the right side of the back panel. Important: Only use the power adapter provided with the DML 3000. Use of a power adapter other than the one provided with the DML 3000 voids the warranty.
  • Page 41 COM port to use with the HC2 System PC. If a RCS or LIS is used on the same computer as the DML 3000, then a USB- to-serial converter will be required. The USB-to-Serial converter is supplied HC2 System with the Personal Computer Country Kit.
  • Page 42 Installation Example of the DML 3000 with the HC2 System PC: DML 3000 User Manual...
  • Page 43 Installation Diagram of connecting a DML 3000 with a RCS: DML 3000 User Manual...
  • Page 44 Installation Diagram of connecting a DML 3000 with a LIS: DML 3000 User Manual...
  • Page 45 Installation Diagram of connecting a DML 3000 with a RCS and a LIS: DML 3000 User Manual...
  • Page 46 Installation This page intentionally left blank DML 3000 User Manual...

  • Page 47: General Operation

    A mechanical test has been performed. Moving the transport locks to the home position The transport locks must be located in the home position to operate the DML 3000. Use this procedure to move into the transport locks to the home position.

  • Page 48: Placing A Microplate In The Dml 3000

    General Operation Slide the transport lock on the left guide rail to the front of the DML 3000 and tighten the thumb screw. View of the transport locks in the home position: Placing a microplate in the DML 3000 Open the access door.
  • Page 49 The microplate locator must retract, and the microplate must be flush against the plate carrier. Make sure the microplate is not resting on top of the microplate locator. Example of the microplate in the plate carrier: Close the plate mask. DML 3000 User Manual...

  • Page 50: Securing The Dml 3000 Using The Transport Locks

    Slide the transport lock on the optical head guide rail all the way to the right, and tighten the thumb screw. Slide the transport lock on the left guide rail to the back of the DML 3000, and tighten the thumb screw.

  • Page 51: Transporting The Dml 3000

    Open the access door. Gently push the plate carrier to the back of the DML 3000. View of the plate carrier pushed to the back of the DML 3000: Place the foam-block insert into the space between the plate carrier guide rails. If...
  • Page 52 CAUTION Damage to the instrument If the internal components of the DML 3000 are not secured, severe damage to the DML 3000 will result and the warranty will be voided. Once the internal components are secure, close the access door.
  • Page 53 General Operation Place the DML 3000 in the original cardboard shipping box. Example of placing the DML 3000 in the shipping box: Place the RS-232 cable, power adapter, and power cord in the shipping box. Note: Do place the USB-to-Serial connector in the shipping box.
  • Page 54 General Operation This page intentionally left blank DML 3000 User Manual...

  • Page 55: Maintenance

    7.1.1 Cleaning the exterior of the DML 3000 At least once per week, wipe the exterior of the DML 3000 with a low-lint paper towel moistened with 70% isopropyl alcohol or 0.5% bleach solution. If a 0.5% bleach solution is used, follow by wiping with a low-lint paper towel moistened with deionized or distilled water.
  • Page 56 If liquid has spilled inside the DML 3000 or liquid is visible on the plate mask or the underside of the plate mask, wipe up the excess liquid with a low-lint paper towel, and clean the interior of the instrument as described below.
  • Page 57 Maintenance Example of wiping the interior of the plate mask: DML 3000 User Manual...
  • Page 58 Maintenance Clean each opening in the plate mask with a foam-tipped applicator or cotton swab wrapped in a low-lint paper towel moistened with 70% isopropyl alcohol. Example of cleaning the plate mask openings: DML 3000 User Manual...
  • Page 59 Maintenance Gently push the plate carrier to the back of the DML 3000. Example of gently pushing the plate carrier: DML 3000 User Manual...
  • Page 60 Maintenance Gently push the optical head from the home position toward the center of the instrument. Example of the optical head in the home position: DML 3000 User Manual...
  • Page 61 Push up on the left side of the optical mask retainer to detach it from the left pin, and then pull the optical mask retainer to the left to detach it from the right pin. Example of removing the optical mask retainer: DML 3000 User Manual...
  • Page 62 Rinse the optical mask and optical mask retainer with deionized or distilled water. Allow the optical mask and optical mask retainer to air dry completely before returning to the instrument. DML 3000 User Manual...

  • Page 63: Decontaminating The Dml 3000

    To decontaminate the DML 3000, clean the interior of the instrument. See “Cleaning the interior of the DML 3000,” page 7-1, for additional instructions. Once the cleaning is complete, wait at least 10 minutes before wiping the interior of the instrument with a low-lint paper towel moistened with deionized or distilled water.
  • Page 64 Maintenance This page intentionally left blank 7-10 DML 3000 User Manual...

  • Page 65: Troubleshooting

    Make sure the correct COM port is used for the connections. Refer to digene System Software User Manual for additional information. Check the serial cabling from the DML 3000 to the computer and make sure the connection is tight. digene Correct the “Instrument Type”...

  • Page 66: Door Is Open" Message

    The DML 3000 is powered ON but has not warmed up High background condition If the DML 3000 is not powered ON at all times, allow the DML 3000 to warm up for at least an hour prior to microplate measurement.

  • Page 67: Technical Data

    Physical characteristics Characteristic Parameter Dimensions (h x w x d)) 26.1 x 49.3 x 51.0 cm (10.3 x 19.4 x 20.0 in.) Height of DML 3000 with access 51 cm (20 in.) door open Weight 10 kg (22 lbs) Operating Conditions...
  • Page 68 Appendix A Transport conditions Condition Parameter Air temperature −20°C to 60°C (–4°F to 140°F) in manufacturer’s package Storage conditions Condition Parameter Air temperature −20 to 40°C (–4 to104°F) in manufacturer’s package DML 3000 User Manual...

  • Page 69: Dml 3000 Maintenance Log

    Appendix B DML 3000 Maintenance Log Use this log to record routine maintenance of the DML 3000 according to your laboratory’s policies and procedures. As needed and weekly maintenance of DML 3000 Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date As Needed...
  • Page 70 Appendix B Monthly maintenance of DML 3000 Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date Monthly Clean the plate mask, plate mask openings, and the plate carrier Clean the optical mask and optical mask retainer Clean the black metal shelf Clean the guide rails...

  • Page 71: Waste Electrical And Electronic Equipment (Weee

    WEEE-marked electronic equipment is provided. To recycle electronic equipment, contact your local QIAGEN sales office for the required return form. Once the form is submitted, you will be contacted by QIAGEN either to request follow-up information for scheduling collection of the electronic waste or to provide you with an individual quote.
  • Page 72 Appendix C This page intentionally left blank DML 3000 User Manual...
  • Page 73 Consult the dealer or an experienced radio or television technician for help. QIAGEN is not responsible for any radio television interference caused by unauthorized modifications of this equipment or the substitution or attachment of connection cables and equipment other than those specified by QIAGEN. The correction of interference caused by such unauthorized modification, substitution, or attachment will be the responsibility of the user.
  • Page 74 Appendix D This page intentionally left blank DML 3000 User Manual...

  • Page 75: Index

    Transport Gas actuator lift arm, 3-5 Caution, 2-2, 6-6 Installation Transport locks, 3-14 Requirements, 1-2 Transportation LED status lights, 3-3 Requirements, 1-2 Maintenance USB-to-Serial converter, 3-19 Log, B-1 Voltage fluctuations, A-1 Requirements, 1-2 Warning symbol, 2-1 DML 3000 User Manual...
  • Page 76 Index Waste electrical and electronic equipment, Index-2 DML 3000 User Manual...
  • Page 77 Appendix D This page intentionally left blank DML 3000 User Manual...
  • Page 78 Index This page intentionally left blank Index-4 DML 3000 User Manual...
  • Page 79 Appendix D This page intentionally left blank DML 3000 User Manual...
  • Page 80 Sample & Assay Technologies 1058536CS Rev. 01 04/2012...
  • Page 81 Australia techservice-au@qiagen.com Austria techservice-at@qiagen.com Belgium techservice-bnl@qiagen.com Brazil suportetecnico.brasil@qiagen.com Canada techservice-ca@qiagen.com China techservice-cn@qiagen.com Denmark techservice-nordic@qiagen.com Finland techservice-nordic@qiagen.com France techservice-fr@qiagen.com Germany techservice-de@qiagen.com Hong Kong techservice-hk@qiagen.com India techservice-india@qiagen.com Ireland techservice-uk@qiagen.com Italy techservice-it@qiagen.com Japan techservice-jp@qiagen.com Korea (South) techservice-kr@qiagen.com Luxembourg techservice-bnl@qiagen.com Mexico techservice-mx@qiagen.com The Netherlands techservice-bnl@qiagen.com...

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