Qiagen PyroMark Q96 ID User Manual page 123

Index
A
Analyzing the run, 5-18
Annealing of sequencing primer to
samples, 5-14
Assay
design and validation, 1
setup, 5
Assembly of the PyroMark Q96 Vacuum
Workstation, 5-7
D
DNA amplification, 5-7
F
Filter probes
function test, 7-9
replacement, 7-10
G
General Description, 3-1
features, 3-5
Pyrosequencing principle, 3-3
Glossary, 9-1
I
Immobilization of the PCR product to
beads, 5-8
Informations de sécurité, 1
Installation
grounding requirements, 4-2
moving the instrument, 4-3
power requirements, 4-2
site requirements, 4-1
Intended use, 2-3
L
Liability clause, 1
PyroMark Q96 ID User Manual 01/2016
M
Maintenance
changing the fuses, 7-4
cleaning the cartridge, 7-5
cleaning the heating block, 7-3
cleaning the lens array, 7-3
cleaning the vacuum workstation, 7-8
routine, 7-2
O
Operating Procedures, 5-1
finishing work, 5-23
managing instrument parameters, 5-4
post-run procedures, 5-18
run, 5-15
setting up a run, 5-3
setting up an assay, 5-2
starting the system, 5-1
P
PCR, 1
optimization, 3
setup, 2
Q
Quality assessments, 8
R
Reagent preparation, 5-14
S
Safety Information, 1-1
chemical hazards, 1-3
electrical safety, 1-2
heat hazards, 1-4
mechanical hazards, 1-3
proper use, 1-1
Sample preparation, 5-6
Appendix E
E-1