Annealing Of Sequencing Primer To Samples; Reagent Preparation - Qiagen PyroMark Q96 ID User Manual

Operating Procedures
5.4.5

Annealing of sequencing primer to samples

WARNING Hot surface
The thermoplate and the heating block (for annealing) can
reach temperatures of up to 80°C (176°F). Avoid touching
them when they are hot.
1. Place the PyroMark Q96 Plate Low in a prewarmed
2. Remove the plate from the thermoplate and allow the
5.4.6

Reagent preparation

1. Prepare reagents according to instructions included with
2. Open a reagent cartridge pack and take out a new
3. Remove the plastic needle cover from the underside of
4. Place the reagent cartridge with the label facing towards
5-14
PyroMark Q96 Sample Prep Thermoplate Low, and heat
the samples on a heating block at 80°C for 2 min.
samples to cool to room temperature (15–25°C) for at
least 5 min. The plate can now be processed in the
PyroMark Q96 ID.
the PyroMark Gold Q96 Reagents.
cartridge. The cartridge has to be handled with care in
order to maintain accuracy.
the reagent cartridge. Save the cover for use when
disposing of the reagent cartridge. Do not use the plastic
needle cover between runs, since applying and removing
the cover several times may damage the needles.
Note: If re-using a reagent cartridge, ensure that it is
cleaned appropriately, see Section 7.6. If handled
correctly, a reagent cartridge can be used for up to
20 runs.
Note: It is important that the reagents are pipetted into
the correct reagent cartridge compartments.
you. Pipet the recommended volume of substrate solution
into the S compartment of the reagent cartridge.
PyroMark Q96 ID User Manual 01/2016
[W8]