Qiagen PyroMark Q96 ID User Manual page 80

Glossary
b) Results only in some
wells of the plate
8.2 Sequence errors
a) Some peaks
appear, but the
sequence stops
8-2
Comments and suggestions
Insufficient sample amounts. Follow sample
preparation instructions carefully (see Section
5.4).
Wells marked in the software do not correspond
to the sample placement in the PyroMark Q96
Plate Low. Check the plate was loaded according
to the information under the "Sample" tab in the
"Run Setup" dialog.
Insufficient enzyme or substrate for all the wells.
Check the reagent cartridge was filled according
to the "Volume Information" under the "Run Info"
tab.
Comments and suggestions
Ensure that all nucleotide chambers in the reagent
cartridge contain nucleotides. Check the reagent
cartridge for blockages and rinse or replace it, as
necessary.
Check the sample and sample preparation.
Contaminated samples will lead to an unusually
high consumption of substrate at substrate
addition, giving a high presequencing signal and
no light produced at nucleotide dispensation.
PyroMark Q96 ID User Manual 01/2016