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b) Faulty or poor
sequence
8.3
Peak errors
a) Wide peaks
PyroMark Q96 ID User Manual 01/2016
Comments and suggestions
Contaminated or nonhomogeneous samples.
Follow sample preparation instructions carefully,
see Section 5.4.
Reagents incorrectly stored or dissolved.
Too much or too little template.
Wrong sequencing primer added.
Wrong sequence to analyze entered.
Wrong dispensation order chosen.
Comments and suggestions
Assay-specific problem due to secondary
structure.
Contaminated or nonhomogeneous samples.
Follow sample preparation instructions carefully,
see Section 5.4.
Reagents incorrectly stored or dissolved.
Too much or too little template.
Too much template DNA. Check amount of DNA
template used.
Reaction volume in wells was too high. Use lower
volume.
Glossary
8-3
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