Wright Giemsa Method - Siemens ADVIA 2120 Operator's Manual

2. White WL, Erickson MM, Stevens SC. Practical Automation for the Clinical
Laboratory. St. Louis, MO, CV Mosby Co., pp 476-487 (1972)
3. Moss ED. Automated Slide Staining in Haematology. Can. J. Med. Tech. 30
(5): 169 (1968)

Wright Giemsa Method

Intended Use
The Wright Giemsa method is for in vitro diagnostic use in the differential
staining of blood, bone marrow and body fluid smears on the ADVIA Autoslide
Slide Maker Stainer. Stained smears are evaluated to identify and quantify cells
in whole blood and other specimens to aid clinicians in the assessment of various
clinical conditions.
Summary and Explanation
The reagent protocol for the Autoslide Slide Maker Stainer consists of a modified
polychrome methylene blue-eosin stain and is based on the original stain
proposed by Romanowsky.
The ADVIA Autoslide Slide Maker Stainer is a smart, fully automated system. It
is capable of producing stained smears on all specimens run on the ADVIA
2120/2120i or only on samples with predefined criteria (i.e. flags or parameter
threshold). The Autoslide also provides a direct access port to the stainer for pre-
smeared slides.
Staining Protocol
The following is a brief description of the staining protocol:
1. Dispensing Modified Wright or Wright-Giemsa Stain (DN1).
2. Slide introduction.
3. Draining Modified Wright or Wright-Giemsa Stain (AN3).
4. Dispensing diluted Modified Wright or Wright-Giemsa Stain (DN3).
5. Draining (AN7).
6. Dispensing rinsing solution (distilled water) (DN7).
10-32 ADVIA Autoslide Slide Maker Stainer