Bibliography; Calculating Retic Parameters - Siemens ADVIA 2120 Operator's Manual

Using the Mie theory of scattering for homogeneous spheres , the low-angle and
high-angle light scatter signals for each cell are transformed into volume and
hemoglobin concentration values. RBCs have cell volumes between 30 fL and
180 fL, and hemoglobin concentrations between 19 g/dL and 49 g/dL.

Bibliography

Tycko DH, Metz MH, Epstein EA, Grinbaum: Flow-cytometric light scattering
measurement of red blood cell volume and hemoglobin concentration. Applied
Optics 24(9):1355-1365 (1985)

Calculating Retic Parameters

Reported Parameters
Parameter
#Retic
%Retic
CHr
System Parameters
The following parameters are for research or laboratory use only and are not for
patient reporting:
Parameter
RTC RBC
RTC Flatness
RTC % Dead Time
8-66
Explanation
RBC x (%Retic ÷ 100) x 1000
100 x (RETIC Count ÷ #RTC Gated Cells) x %
RETIC Cal Factor
Mean of the RETIC CH histogram for the
reticulocyte population
Explanation
RBC Count
Sum of theSquared Differences
9 ×
Mean Cell Counting Rate
100 x (Measured Dead Time ÷
Measured Sample Time)
Methods