Siemens ADVIA 2120 Operator's Manual page 372

The following Regression statistics were computed using a protocol similar to
that recommended in the NCCLS document EP9-A, Method Comparison and
Bias Estimation Using Patient Samples; Approved Guideline (1995).
Parameter
WBC (10³/µL) Technicon H•3 RTC
RBC (10
HGB (g/dL)
MCV (fL)
CH (pg)
CHCM (g/dL) Technicon H•3 RTC
RDW (%)
HDW (g/dL)
PLT (10³/µL)
MPV (fL)
x = Technicon H•3 RTC system
y = ADVIA 120 Hematology system
Performance Characteristics: Carryover
Sample carryover for the ADVIA 120 Hematology System was measured for
WBC, RBC, HGB, and PLT. The performance specification for carryover is ≤
1% for all parameters.
Carryover was evaluated on the ADVIA 120 Hematology System by the
aspiration of a high pool prepared for linearity testing, followed by 3 aspirations
of cell-free plasma. Percent carryover was calculated as:
% Carryover = [(L
L
1
L
3
H
The following results were obtained:
Parameter
WBC
RBC
HGB
Regulatory Information
Comparative System
or Method
6
/µL) Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
Technicon H•3 RTC
- L ) / H] x 100
1 3
= the count for the first aspiration of cell-free plasma
= the count for the third aspiration of cell-free plasma
= the count from the high pool
Observed Carryover
0.4%
0.2%
0.2%
Regression
Equation (y=) r
1.06x - 0.74 0.997 1.42
0.98x + 0.11 0.997 0.07
1.01x - 0.2 0.997 0.2
0.90x + 9.6 0.994 1.1
1.00x + 1.2 0.990 0.6
1.17x - 6.5 0.974 0.5
0.86x + 1.3 0.980 0.6
0.98x + 0.06 0.959 0.13
1.08x - 2.0 0.991 27
0.76x + 0.9 0.925 0.4
Sy.x
9-31