Sample Handling - Siemens ADVIA 2120 Operator's Manual

Sample Handling

Sample Collection
Collect whole blood in an evacuated blood collection tube containing EDTA as
an anticoagulant. Blood samples should be refrigerated at a temperature between
2°C to 8°C if they will not be analyzed within eight (8) hours of phlebotomy. If a
specimen has been refrigerated, allow it to equilibrate slowly to room
temperature (15°C to 30°C) before mixing.
Sample Dilution
Whole blood specimens whose parameter values exceed the analytical range may
be diluted with autologous plasma or isotonic saline and reassayed.
Sample Stability
The effect of the aging of blood was studied over a 72-hour period on the
ADVIA 120 Hematology System.
Two whole-blood specimens drawn from 15 normal, apparently healthy donors
were assayed shortly after phlebotomy and then again at intervals of 8, 24, 36,
48, 56, and 72 hours.
One of the whole-blood specimens from each pair was stored at room
temperature while the corresponding specimen was stored at 2°C to 8°C in
capped blood-collection tubes that contained EDTA as the anticoagulant.
The results indicate that CBC parameters are stable within two (2) standard
deviations (within run precision) of the initial recovery for the specified time
interval. The stability of the calculated parameters is limited to the stability of the
least stable primary parameter.
Parameter
WBC (10³/µL)
RBC (10
HGB (g/dL)
MCV (fL)
CH (pg)
CHCM (g/dL)
RDW (%)
HDW (g/dL)
PLT (10³/µL)
MPV (fL)
Regulatory Information
Room Temperature
Stability (hours)
36
6
/µL) 48
72
8
36
8
72
72
48
8
Refrigerated Temperature
Stability (hours)
56
72
72
24
53
24
72
72
48
8
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