Using the Mie theory of light scattering for homogeneous spheres, the low-angle,
high-gain light scatter measurement is converted into cell volume and the high-
angle, high-gain light scatter measurement is converted into refractive index (n).
The following histograms and cytograms are used for platelet analysis:
Platelet X
scatter signals.
Platelet Y
scatter signals.
Platelet Vol
Platelet PM
Platelet PC
PLT Scatter
light scatter signals along the x axis and the low-angle (2° to 3°), high-gain light
scatter signals on the y axis.
PLT Volume PC
(PC) along the x axis and the platelet volume on the y axis.
Bibliography
Tycko DH, Metz MH, Epstein EA, Grinbaum: Flow-cytometric light scattering
measurement of red blood cell volume and hemoglobin concentration. Applied
Optics 24(9):1355-1365 (1985)
Methods
histogram is formed from the high-angle (5° to 15°), high-gain light
histogram is formed from the low-angle (2° to 3°), high-gain light
histogram shows the distribution of platelets by cell volume.
histogram shows the distribution of platelets by dry mass.
histogram shows the distribution of platelets by refractive index.
cytogram is formed by plotting the high-angle (5° to 15°), high-gain
cytogram is formed by plotting the refractive index of platelets
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