Measurement; Baso Rate Histogram - Siemens ADVIA 2120 Operator's Manual

•
Prevents contact between the sample stream and the flowcell walls, which
prevents clogs and staining of the flowcell
•
Provides an optically transparent medium through which the sample stream
can be clearly focused
Rinse for Baso, RBC / Platelet, Peroxidase, Reticulocyte, and Hemoglobin
Methods
•
Cleans the hydraulic pathways for each method
Baseline solution for Hemoglobin Method
•
Provides a baseline reading

Measurement

A constant volume of the cell suspension from the baso reaction chamber passes
through the flowcell where the low-angle light scatter (2° – 3°) and high-angle
light scatter (5° – 15°) signatures of each white blood cell are measured, based
on:
•
The size of the cell or nucleus
•
The nuclear configuration, which is a combination of the nuclear shape and
cell density
ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two
fluids pass through the flowcell.
The optical signals from the flowcell are converted to electrical pulses by
photodiodes. After processing, the following information is available:
BASO Rate
BASO X
configuration) for the MN (mononuclear) and PMN (polymorphonuclear)
populations.
BASO Y
MN population.
BASO
Cluster analysis identifies the individual populations.
If cluster analysis cannot be used for a particular sample, the cell populations are
identified using histogram analysis.

BASO Rate Histogram

8-4
histogram displays the arrival rate of cells in the Baso channel.
histogram displays the high-angle light scatter information (nuclear
histogram displays the low-angle light scatter information (size) for the
cytogram is formed from the paired low and high-angle light scatter data.
The BASO Rate histogram
shows the uniformity of the
cell-counting rate.
Methods